Tissue culture method for pineapples

A tissue culture, pineapple technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low survival rate, limited pineapple cultivation, low reproductive efficiency, etc.

Inactive Publication Date: 2018-03-06
芜湖东源新农村开发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing pineapple propagation methods are commonly used: sowing, branching and budding; no matter the above-mentioned p...

Method used

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  • Tissue culture method for pineapples
  • Tissue culture method for pineapples
  • Tissue culture method for pineapples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1) Sterilize the crown buds of pineapple (first soak the crown buds in 65% by weight alcohol aqueous solution for 40s, then soak them in 0.08% by weight mercuric chloride solution for 6min, and finally wash them with distilled water for 4 times ), then chopped the crown buds as explants (0.7cm in length and 0.2cm in width), and then placed the explants in basal medium for initial induction (first cultured in dark for 1.5 days , and then transferred to the condition of alternating light and dark for 5 days; wherein, the condition of alternating light and dark is: the light time is 13h / day, the dark time is 11h / day, the light intensity is 2300lux, the culture temperature is 22°C, and the relative humidity is 55%) to obtain callus;

[0041] 2) The callus is placed in the induction medium for secondary induction (under the condition of alternating light and dark until the height of the adventitious bud exceeds 2cm; wherein, the condition of alternating light and dark is: the ...

Embodiment 2

[0047] 1) Sterilize the crown buds of pineapple (first soak the crown buds in 60-70% by weight alcohol aqueous solution for 30-60s, then soak them in 0.1% by weight mercuric chloride solution for 7min, and finally pass them through distilled water wash 3-5 times), then chop the crown buds as explants (0.6 cm in length and 0.2 cm in width), and then place the explants in basal medium for initial induction (Cultivate in the dark for 1 day, and then transfer to the condition of alternating light and dark for 4-6 days; wherein, the condition of alternating light and dark is: the light time is 12h / day, the dark time is 10h / day, and the light intensity is 2300lux. The temperature is 21°C, the relative humidity is 50%) to obtain callus;

[0048] 2) The callus is placed in the induction medium for secondary induction (under the condition of alternating light and dark until the height of the adventitious bud exceeds 2cm; wherein, the condition of alternating light and dark is: the ligh...

Embodiment 3

[0054] 1) Sterilize the crown buds of pineapple (first soak the crown buds in 70% by weight alcohol aqueous solution for 60s, then soak them in 0.15% by weight mercuric chloride solution for 8min, and finally wash them with distilled water for 5 times ), then chopped the crown buds as explants (length 0.8cm, width 0.3cm), and then placed the explant explants in basal medium for initial induction (first dark culture for 2 days , and then transferred to the condition of alternating light and dark for 6 days; wherein, the condition of alternating light and dark is: the light time is 14h / day, the dark time is 12h / day, the light intensity is 2400lux, the culture temperature is 23°C, and the relative humidity is 60%) to obtain callus;

[0055] 2) The callus is placed in the induction medium for secondary induction (under the condition of alternating light and dark until the height of the adventitious bud exceeds 2cm; wherein, the condition of alternating light and dark is: the light...

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Abstract

The invention discloses a tissue culture method for pineapples. The tissue culture method comprises the following steps: (1) sterilizing and disinfecting crown buds of the pineapples, cutting the crown buds up to serve as an explant, putting the explant into a basic culture medium for primary induction, so as to obtain a callus; (2) putting the callus into an induction culture medium for secondaryinduction, so as to hasten the generation of adventitious buds; (3) dicing the callus with the adventitious buds so as to obtain a callus block with at least two adventitious buds, putting the callusblock into a proliferation culture medium for proliferation culture, so as to obtain a proliferated bud block; (4) putting the proliferated bud block into a rooting culture medium for rooting culture, so as to obtain a tissue culture seedling; and (5) hardening the tissue culture seedling. The tissue culture method for the pineapples has excellent survival rate and reproduction rate.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a pineapple tissue culture method. Background technique [0002] Pineapple: scientific name pineapple, short stem, many leaves, rosette arrangement, sword-shaped, apex acuminate, entire margin or sharp teeth, green belly, pink-green back, often maroon on the edge and top, leaves born on the top of the inflorescence Smaller, often red. The inflorescence is pulled out from the leaves, shaped like a pine cone; the base of the bracts is green, the upper part is light red, and triangular-ovate; the sepals are broadly ovate, fleshy, and the top is reddish; the petals are oblong, with apex, and the upper part is purple-red , the lower part is white. Juhua fruit fleshy. Flowering period from summer to winter. Pineapple is one of the famous tropical fruits. It is native to the tropical regions of America. Now it is also cultivated in Fujian, Guangdong, Hainan, Guangxi, and Yunnan in my count...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 汪锡文裴宝平邱许凤
Owner 芜湖东源新农村开发股份有限公司
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