Tissue culture method of trichosanthes kirilowii
A technology of tissue culture and melon, applied in the field of tissue culture of melon, can solve the problems of destruction, the output cannot meet market requirements, the limited wild resources of melon, etc.
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[0034] Example 1
[0035] 1) Cut the stems of 0.3cm in length as explants for heat treatment and sterilization (sterilization temperature is 46℃, sterilization time is 5min), and then placed in alcohol solution (ethanol concentration is 60-70 volume) %) in the secondary sterilization (sterilization time is 20min, sterilization temperature is 20 ℃), and then placed in the primary culture medium for induction culture (first dark culture for 1.5 days, then switch to the light and dark alternate conditions 5 days; among them, the light and dark alternate conditions are: the light time is 13h / day, the light intensity is 1500lux, the culture temperature is 28℃, the relative humidity is 55%), the proliferation culture is carried out in the proliferation medium (under the alternate light and dark conditions) Cultivate for 9 days under the conditions of light and dark alternate conditions: light time is 13h / day, light intensity is 1800lux, culture temperature is 22℃, relative humidity is ...
Example Embodiment
[0039] Example 2
[0040] 1) Cut the 0.25cm length of Gualou stems as explants for heat treatment and sterilization (the sterilization temperature is 42°C, and the sterilization time is 6min), and then placed in an alcohol solution (the ethanol concentration is 60% by volume) Carry out secondary sterilization in medium (sterilization time is 15min, sterilization temperature is 35℃), and then placed in primary culture medium for induction culture (first dark culture for 1 day, then switch to light and dark alternate conditions for 4 days ; Among them, the light and dark alternate conditions are: the light time is 12h / day, the light intensity is 1400lux, the culture temperature is 25℃, the relative humidity is 50%), the propagation culture is carried out in the proliferation medium (cultivation under alternate light and dark conditions For 8 days, the light and dark alternate conditions are: the light time is 12h / day, the light intensity is 1700lux, the culture temperature is 20℃, ...
Example Embodiment
[0044] Example 3
[0045] 1) Cut the 0.35cm Gualou stem as explants for heat treatment and sterilization (sterilization temperature is 48℃, sterilization time is 3min), and then placed in alcohol solution (ethanol concentration is 70% by volume) Carry out secondary sterilization in medium (sterilization time is 25min, sterilization temperature is 35℃), and then placed in primary culture medium for induction culture (first dark culture for 2 days, then switch to light and dark alternate conditions for 6 days ; Among them, the alternate light and dark conditions are: the light time is 14h / day, the light intensity is 1600 lux, the culture temperature is 30 ℃, the relative humidity is 60%), the proliferation culture in the proliferation medium (cultivation under alternate light and dark conditions) For 10 days, the light and dark alternate conditions are: light time is 14h / day, light intensity is 1900lux, culture temperature is 25℃, relative humidity is 60%) to obtain differentiated ...
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