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Tissue culture method of trichosanthes kirilowii

A technology of tissue culture and melon, applied in the field of tissue culture of melon, can solve the problems of destruction, the output cannot meet market requirements, the limited wild resources of melon, etc.

Inactive Publication Date: 2018-12-07
芜湖东源新农村开发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the wild resources of Trichosanthes quinquefolius are limited and severely damaged. Although there are a small number of introduced and cultivated varieties in various places, their output is far from meeting market requirements.

Method used

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  • Tissue culture method of trichosanthes kirilowii
  • Tissue culture method of trichosanthes kirilowii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1) Cut the stems of Trichosanthes mellifera with a length of 0.3 cm as explants for heat treatment and degerming (the degerming temperature is 46°C, and the degerming time is 5 minutes), and then placed in alcohol solution (the concentration of ethanol is 60-70 volume %) for secondary sterilization (sterilization time is 20min, sterilization temperature is 20°C), and then placed in the primary culture medium for induction culture (first cultured in dark for 1.5 days, then transferred to light-dark alternating conditions) 5 days; among them, the conditions of alternating light and dark are: the light time is 13h / day, the light intensity is 1500lux, the culture temperature is 28°C, the relative humidity is 55%), and the proliferation culture is carried out in the proliferation medium (in the condition of light and dark alternation Under culture for 9 days, the conditions of alternating light and dark are: the light time is 13h / day, the light intensity is 1800lux, the cultu...

Embodiment 2

[0040] 1) Take the 0.25cm-long Trichosanthes stem section as an explant for heat treatment and degerming (the degerming temperature is 42°C, and the degerming time is 6 minutes), and then placed in an alcohol solution (the concentration of ethanol is 60% by volume) Secondary sterilization (sterilization time: 15min, sterilization temperature: 35°C), and then placed in the primary culture medium for induction culture (first dark culture for 1 day, then transfer to light and dark conditions for 4 days) ; Among them, the conditions of alternating light and dark are: the light time is 12h / day, the light intensity is 1400lux, the culture temperature is 25°C, and the relative humidity is 50%); 8 days, alternating light and dark conditions: the light time is 12h / day, the light intensity is 1700lux, the culture temperature is 20°C, and the relative humidity is 50%) to obtain differentiated seedlings;

[0041] 2) Carry out rooting culture of differentiated seedlings in rooting medium (...

Embodiment 3

[0045] 1) Cut the stems of Trichosanthes mellifera with a length of 0.35 cm as explants for heat treatment and degerming (the degerming temperature is 48°C, and the degerming time is 3 minutes), and then placed in alcohol solution (the concentration of ethanol is 70% by volume) Secondary sterilization (sterilization time: 25min, sterilization temperature: 35°C), and then placed in the primary culture medium for induction culture (first dark culture for 2 days, then transfer to light and dark conditions for 6 days) ; Among them, the light-dark alternation conditions are: the light time is 14h / day, the light intensity is 1600lux, the culture temperature is 30°C, and the relative humidity is 60%); 10 days, alternating light and dark conditions: the light time is 14h / day, the light intensity is 1900lux, the culture temperature is 25°C, and the relative humidity is 60%) to obtain differentiated seedlings;

[0046] 2) Carry out rooting culture of differentiated seedlings in rooting ...

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Abstract

The invention discloses a tissue culture method of trichosanthes kirilowii, which includes: 1) performing thermal treatment sterilization to trichosanthes kirilowii stem sections being an explant, performing secondary sterilization in alcohol solution, and performing induction culture in a primary culture medium and then proliferation culture in a proliferation culture medium to obtain differentiated seedlings; 2) performing rooting culture on the differentiated seedlings in a rooting culture medium, then performing low temperature treatment at lower than 5 DEG C to obtain tissue culture seedlings; 3) exercising the tissue culture seedlings. The primary culture medium is a MS culture medium containing naphthylacetic acid (NAA), 6-benzyladenine (6-BA) and inositol; the proliferation culturemedium is a MS culture medium containing zeatin (ZT), kanamycin, 2,4-D and tomato juice; and the rooting culture medium is a 1 / 2 MS culture medium containing paclobutrazol, indolebutyric acid (IBA) and smashed banana. The method has excellent survival rate and reproductive rate.

Description

technical field [0001] The invention relates to tissue culture, in particular to a method for tissue culture of Trichosanthes trichosanthes. Background technique [0002] Trichosanthes, also known as Cucurbitaceae, is a perennial climbing herb with a length of up to 10 meters. The rhizome is thick, cylindrical, and the outer skin is yellow. Stem much branched, glabrous; leaves alternate, nearly round or heart-shaped, dioecious; male flowers several racemes, rarely solitary, corolla lobes obovate, female flowers solitary, ovary ovate, fruit nearly spherical, Orange-red when ripe, flower and fruit period July-November. Gualou is distributed in Liaoning, North China, East China, Central South China, Shaanxi, Gansu, Sichuan, Guizhou and Yunnan. Born in hillside forests, bushes, grasslands and fields beside villages at an altitude of 200-1800 meters. Because this species is the traditional Chinese medicine Trichosanthes trichosanthes and Trichosanthes chinensis, it is widely ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 汪锡文裴宝平邱许凤
Owner 芜湖东源新农村开发股份有限公司
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