The invention discloses hydroponic nutrient solution of water spinach. The nutrient solution is prepared from macroelements, microelements and water, wherein the nutrient solution comprises the macroelements, such as carbon, hydrogen, oxygen, sulfur, nitrogen, phosphorus, potassium, calcium and magnesium, and the microelements, such as iron, boron, manganese, zinc, chlorine, copper, aluminum, molybdenum, cobalt, silicon and sodium. The main ingredients of the hydroponic nutrient solution of the water spinach include sodium silicate, zinc sulfate, calcium nitrate terahydrate, potassium nitrate, magnesium sulphate pentahydrate, calcium chloride, sodium molybdate, ethylene diamine tetraacetic acid, zinc sulphate, sodium chloride, boric acid, manganese sulfate tetrahydrate, blue copperas, nopotassium phosphate, cobalt chloride, aluminum nitrate and ferric sulfate. The hydroponic nutrient solution of water spinach is high in absorption and utilization rate; and growth of the water spinach is accelerated. Thus, waste caused by nutrition excess or shortage due to the fact that the hydroponic nutrient solution of the water spinach has no pertinence for a long period of time is radically solved.