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Fresh ginger tissue culture method

A technology of tissue culture and rooting medium, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of difficult to use sexual reproduction, high cost, low reproduction coefficient, etc., and achieve excellent survival rate and The effect of high yield

Inactive Publication Date: 2018-11-16
芜湖东源新农村开发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because ginger does not bloom or rarely blooms, it is difficult to use sexual reproduction in breeding, but can only rely on a few varieties for long-term asexual reproduction, resulting in the accumulation of various viruses in the ginger plant, resulting in decreased yield, quality degradation, and decreased stress resistance.
The reproduction and preservation of ginger germplasm resources also mainly rely on planting ginger in the field, which not only has a low reproduction coefficient and high cost, but also easily leads to the loss of resource materials

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0034] 1) Ginger bud tips with a length of 0.3 cm were used as explants for heat treatment and degerming (the degerming temperature was 45°C, and the degerming time was 5 min), and then placed in an antibiotic solution (containing 0.9 g / L penicillin and 0.3 g / L Lgentamicin) for secondary sterilization (sterilization time: 20min, sterilization temperature: 20°C), and then placed in the primary culture medium for induction culture (first cultured in dark for 1.5 days, then transferred to alternate light and dark cultured under the conditions of 5 days; among them, the alternating light and dark conditions are: the light time is 13h / day, the light intensity is 1500lux, the culture temperature is 28°C, the relative humidity is 55%), and the proliferation culture is carried out in the proliferation medium (in Cultivate under alternating light and dark conditions for 9 days, and the alternating light and dark conditions are as follows: the light time is 13h / day, the light intensity i...

Embodiment 2

[0039]1) Ginger bud tips with a length of 0.25 cm were used as explants for heat treatment and degerming (the degerming temperature was 42°C, and the degerming time was 6 minutes), and then placed in an antibiotic solution (containing 0.8g / L penicillin and 0.2g / L Lgentamicin) for secondary sterilization (sterilization time 15min, sterilization temperature 35°C), and then placed in the primary culture medium for induction culture (first dark culture for 1 day, then transfer to light-dark alternation cultured under the conditions of 4 days; wherein, the alternating light and dark conditions are: the light time is 12h / day, the light intensity is 1400lux, the culture temperature is 25°C, and the relative humidity is 50%), and the proliferation culture is carried out in the proliferation medium (in Cultivate under alternating light and dark conditions for 8 days, and the alternating light and dark conditions are as follows: the light time is 12h / day, the light intensity is 1700lux, ...

Embodiment 3

[0044] 1) Ginger bud tips with a length of 0.35 cm were used as explants for heat treatment for degerming (the degerming temperature was 48°C, and the degerming time was 3 min), and then placed in an antibiotic solution (containing 1.3 g / L penicillin and 0.5 g / L L gentamicin) for secondary sterilization (sterilization time: 25min, sterilization temperature: 35°C), and then placed in the primary culture medium for induction culture (first dark culture for 2 days, then transfer to light-dark alternation cultured under the conditions of 6 days; among them, the alternating light and dark conditions are: the light time is 14h / day, the light intensity is 1600lux, the culture temperature is 30°C, the relative humidity is 60%), and the proliferation culture is carried out in the proliferation medium (in Cultivate for 10 days under alternating light and dark conditions, the alternating light and dark conditions are: the light time is 14h / day, the light intensity is 1900lux, the culture ...

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Abstract

The invention discloses a fresh ginger tissue culture method which comprises the following steps of 1) taking fresh ginger shoot tips as explants to perform bacterium removal with heat treatment, thenputting a product in an antibiotic solution for secondary sterilization, then putting a product in an initial medium for induced culture and in a multiplication medium for multiplication culture to obtain differentiated seedlings; 2) performing rooting culture on the differentiated seedlings in a rooting medium to obtain a tissue culture seedlings; and 3) seedling hardening on the tissue cultureseedlings, wherein the culture conditions of the step 1 to the step 2 at least accord with the following conditions: the condition is aseptic and air permeable; the initial medium is an MS culture medium containing indoleacetic acid (IAA), 6-benzyladenine 6-BA and folic acid; the multiplication culture medium is an MS culture medium containing zeatin ZT, streptomycin, 2,4-D and a watermelon juice;and the rooting medium is a 1 / 2 MS culture medium containing paclobutrazol, indole butyric acid (IBA) and mashed potatoes. The fresh ginger tissue culture method has excellent survive rate and reproductive rate.

Description

technical field [0001] The invention relates to tissue culture, in particular to a ginger tissue culture method. Background technique [0002] Ginger is an important spicy vegetable crop of Zingiberaceae. It is a perennial monocotyledonous herb. It is mostly cultivated as an annual in production. Now it has been widely cultivated in subtropical and tropical regions of the world. Due to its rich nutrition and good medicinal effect, ginger has become an important seasoning vegetable and raw material for medicine, chemical industry and food industry; coupled with its high economic value and resistance to storage and transportation, it has been favored by foreign businessmen in recent years, and its export volume has increased year by year The trend has become one of the main specialty foreign exchange-earning vegetables in my country. [0003] Because ginger does not bloom or rarely blooms, it is difficult to use sexual reproduction in breeding, but can only rely on a few varie...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 汪锡文裴宝平邱许凤
Owner 芜湖东源新农村开发股份有限公司
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