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Artificial seedling breeding technology for Portuguese oyster triploid

A Portuguese oyster and triploid technology, applied in the field of soil remediation, can solve the problems of unable to meet the needs of users, affect the large-scale cultivation of Portuguese oyster triploid, and the low survival rate of Portuguese oyster triploid, and achieve excellent survival rate, The effect of reducing the cost of cultivation and wide application prospects

Pending Publication Date: 2022-06-03
广鸿源(广东)水产有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The patent application No. CN201410197671.1 "a cultivation method for a new strain of Portuguese oyster with golden shell and fast growth" already exists, but the cultivation method provided, when the cultivated Portuguese oyster is triploid, the cultivated Portuguese oyster three The low survival rate of ploidy affects the large-scale cultivation of triploid oysters in Portugal, which cannot meet the needs of users

Method used

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  • Artificial seedling breeding technology for Portuguese oyster triploid
  • Artificial seedling breeding technology for Portuguese oyster triploid
  • Artificial seedling breeding technology for Portuguese oyster triploid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: cultivation of tetraploidy and Diploidium of the Portuguese oyster

[0025] (1) Cultivation of tetraploidy of long oysters

[0026] Take 6-10 cm of unscathed 2-year pure oyster tetraploidy, and select male individuals from the tetraploidy of purebred oysters; Male individuals are placed in the culture tank, and male individuals are provided with temporary salinity of 16-18, temporary breeding temperature of 24-26 ° C, male individuals are bred, during the breeding period, in accordance with the conventional breeding method, monocytobacterial bait is released every day, and the daily amount of bait is 400,000-600,000 / ml;

[0027] (2) Cultivation of diploids of Portuguese oysters

[0028] Female individuals were selected from the diploids of Portuguese oysters over 3 years of age without damage, and then female individuals with a size of 7-8 cm were selected, and the female individuals were placed in the culture tank, and cultivated under the conditions of tempor...

Embodiment 2

[0029] Example 2: Portuguese oyster triploid of the present invention is obtained

[0030] (1) Using artificial dissection, the eggs are removed from the female individuals of the cultivated Portuguese oyster diploid, and filtered with 100 mesh and 200 mesh sieves, respectively, and then filtered with 500 mesh sieves, and then from the male individual tetraploid tetraploid of the long oyster, the sperm is removed, and the sperm is filtered with a 500 mesh sieve silk;

[0031] (2) Observe the sperm under the microscope, select the lively sperm, add the sperm to the egg fluid, and ensure that the ratio of male and female is 30:1, mix sperm and eggs, complete the hybridization of eggs and sperm, that is, complete the fertilization of eggs;

[0032](3) The fertilized eggs are incubated after 20-24h to form D-shaped larvae, and the larvae are selected with a 300 mesh sieve silk to obtain high-quality and robust larvae;

[0033] (4) The preferred larvae are bred into juvenile shells und...

Embodiment 3

[0035] Example 3: Effect of different temperatures on triploid cultivation of Portuguese oysters

[0036] The temporary salinity of the tetraploid cultivation of long oysters is 17, the temporary temperature is 25 °C, the temporary salinity of the Portuguese oyster diploid is 21, the temporary temperature is 25 °C, and then the excellent larvae are cultivated under the condition of salinity of 23 and water temperature of 30 °C, according to the cultivation method of Example 1 and Example 2, 1000 Portuguese oyster triploids are cultivated, as an experimental group, under other conditions remain unchanged, the temporary rearing temperature of the long oyster tetraploid cultivation is 20 °C and 28 °C. Each breeding 1000 Portuguese oyster triploids, as a comparison group 1 and a comparison group 2, other conditions unchanged, the Portuguese oyster diploid cultivation of the temporary rearing temperature of 22 °C and 28 °C, each cultivated 1000 Portuguese oyster triploid, as a comparis...

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Abstract

The invention relates to the technical field of seedling breeding, in particular to a Portuguese oyster triploid artificial seedling breeding technology, which comprises the following steps: hybridizing cultivated crassostrea gigas tetraploid and cultivated Portuguese oyster diploid to obtain Portuguese oyster triploid fertilized eggs, cultivating the Portuguese oyster triploid fertilized eggs to juvenile mollusks, and culturing the juvenile mollusks to obtain the Portuguese oyster triploid artificial seedling. The juvenile mollusks are cultivated into Portuguese oyster triploid adults; proper temperature and salinity are provided for culture of the Portuguese oyster triploid, and the cultured Portuguese oyster triploid has a good survival rate.

Description

Technical field [0001] The present invention relates to the field of soil remediation technology, specifically to portuguese oyster triploid artificial seedling breeding technology. Background [0002] Portuguese oysters, also known as Fujian oysters, belong to the mollusk phylum, petal gills, pearl oysters, oysters, giant oysters, is an important cultured shellfish in South China. Portuguese oysters are not only delicious and nutritious, but also have unique health functions and medicinal value, and are a seafood treasure with high nutritional value. [0003] At present, there are two main ways to obtain the Triploid of the Portuguese oyster, one is to directly induce its triploid, and the other is obtained by hybridization, due to the problem of low success rate of direct induction. Therefore, the cultivation of Portuguese oyster triploids by hybridization became the main way to obtain it. [0004] Patent application no. CN201410197671.1 "A method for the cultivation of a new s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/54
CPCA01K61/54Y02A40/81
Inventor 吴国广吴章继
Owner 广鸿源(广东)水产有限公司
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