Nitrite nitrogen resisting trait related molecular marker of Litopenaeus vannamei and screening of nitrite nitrogen resisting trait related molecular marker of Litopenaeus vannamei

Abstract

The invention discloses a nitrite nitrogen resisting trait related molecular marker of Litopenaeus vannamei and screening of the nitrite nitrogen resisting trait related molecular marker of Litopenaeus vannamei. The SNP marker remarkably related to nitrite nitrogen resistance of the Litopenaeus vannamei is obtained through carrying out a nitrite nitrogen stress experiment on the Litopenaeus vannamei, reserving hepatopancreas of surviving and dead Litopenaeus vannamei as a sample, carrying out PCR amplification by using the sample as a template, sequencing an amplified fragment so as to obtaina partial sequence of a DNA gene of the hepatopancreas and a sequenced peak chart, carrying out BLACT comparison on a sequence result so as to screen out a base mutant site, i.e., a SNP locus, then, carrying out gene subtype analysis on the sequenced peak chart by using software Seqman, and analyzing correlation between the nitrite nitrogen resistance of the Litopenaeus vannamei and a genotype byusing chi-square test of software SPSS19.0. The marker is applied to the selective breeding of the Litopenaeus vannamei and can be used for accurately and efficiently selecting the Litopenaeus vannamei with the nitrite nitrogen resisting trait for large-scale culture.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to molecular markers related to nitrite nitrogen tolerance of Litopenaeus vannamei and screening thereof. Background technique [0002] Litopenaeus vannamei, commonly known as Litopenaeus vannamei, belongs to Crustacea, Decapoda, Penaeidae, Penaeus, and Litopenaeus subgenus. It is native to the Pacific coast of the Americas. It is widely distributed in the coastal waters from southern Peru to Sanola, Mexico. Many, most concentrated in Ecuador. Litopenaeus vannamei has the advantages of large individual, fast growth, strong adaptability, low feed protein requirement, strong disease resistance, long out-of-water survival time, and suitable for long-distance transportation and sales; its meat is tender and delicious, rich in nutrition, and its meat yield is As high as 65%, it is one of the world's three excellent shrimp farming species. Since 1988, my country ha...

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Problems solved by technology

[0003] With the continuous improvement of the breeding level and the increasing of the breeding density, the amount of feed input is large, the accumulation of breeding metabolites, the amount of water exchange is small, and the environmental load is large, resulting in frequent fluctuations in water quality factors and deteriorating stability of the breeding environment, thereby affecting The healthy growth of cultured Litopenaeus vannamei, and high-grade aquatic products such as Litopenaeus vannamei are fed with high protein content in the feed, and the feces produced have high protein content, and the load of the water body mostly reaches or exceeds the saturation level. Under the action of the organic carbon source, the organic carbon source is decomposed in a large amount, and the organic nitrogen source is too much, especially in the condition of insufficient oxygen, the excessive nitrogen source is easily converted into nitrite and ammonia nitrogen, which causes damage to the growth of Litopenaeus vannamei. toxic environment

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