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SNP marker related to growth speed of pigs and application of SNP marker

A growth rate and marker technology, applied in the field of SNP markers, can solve the problems of difficulty in obtaining candidate genes, easily questioned research results, and small sample content, and achieve the effect of improving the genetic level, high sensitivity and high accuracy.

Pending Publication Date: 2020-05-15
广西扬翔股份有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been more and more genome-wide association analyzes of pig growth traits, but most of the studies have a relatively small sample size and are carried out within a single breed. In this case, the research results obtained are easy to be questioned and it is difficult to obtain a broad spectrum. Candidate genes controlling growth traits
[0008] Therefore, current SNP markers for pig growth velocity remain to be studied

Method used

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  • SNP marker related to growth speed of pigs and application of SNP marker
  • SNP marker related to growth speed of pigs and application of SNP marker
  • SNP marker related to growth speed of pigs and application of SNP marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] In this example, refer to figure 1 , by the following method to screen the molecular markers related to pig growth velocity.

[0036] 1. Genotyping detection

[0037] (1) Use the kit method (spin column type) to extract ear tissue or tail tissue DNA from populations related to growth traits

[0038] 1) Cut the ear tissue or tail tissue of the population related to growth traits into a paste with ophthalmic scissors, add 200ul GA, shake and mix well; then put it in a 56°C water bath for overnight digestion.

[0039] 2) Add 200ul buffer GB to the digested tissue sample, mix thoroughly by inversion, and place at 70°C for 10 minutes, the solution should become clear, and briefly centrifuge to remove water droplets on the tube wall.

[0040] 3) Add 200ul of absolute ethanol, shake and mix well for 15 seconds, at this time flocculent precipitation may appear, briefly centrifuge to remove water droplets on the tube wall.

[0041] 4) Add the solution and flocculent precipita...

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Abstract

The present invention provides an SNP marker related to growth speed of pigs. The SNP marker is selected from the 51st nucleotide of a nucleotide sequence shown in SEQ ID NO:1 and is a base C or T. Asa result, the SNP marker can accurately and efficiently breed excellent pig breeds with faster growth speed, has outstanding advantages of simplicity, speediness, high sensitivity, good specificity,etc. and provides a new molecular marker resource and an application for breeding related to the growth speed of the pigs.

Description

technical field [0001] The present invention relates to SNP markers and their applications. Specifically, the present invention relates to SNP markers related to pig growth velocity and applications thereof. Background technique [0002] Domestic pigs are one of the earliest domesticated animals domesticated by humans. Domestic pigs are the main species of meat consumed by humans, and their pork production ranks first among all domestic animals. my country has a large population, and the demand for pork is huge. It is an urgent and important task to carry out genetic improvement of pigs and improve the efficiency of pig breeding. With the development of science and technology, pig breeding has made significant breakthroughs in genetic breeding, nutritional regulation, and disease prevention and control. The growth rate, lean meat rate, and feed meat of commercial pigs have all been greatly improved. [0003] In pig production, growth traits are very important economic trai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11A01K67/02
CPCC12Q1/6888A01K67/02C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 赵书红辛颖李新云刘小磊许静雅刘连超张从林赵志超刘向东
Owner 广西扬翔股份有限公司
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