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A SNP marker associated with low dissolved oxygen tolerance of Litopenaeus vannamei and its screening and application

A vanabine, low dissolved oxygen technology, applied in recombinant DNA technology, biochemical equipment and methods, microbial determination/inspection, etc. The effect of efficiency and accuracy

Inactive Publication Date: 2019-10-15
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above studies mostly focused on the analysis of polymorphisms of genes related to growth and disease resistance of Litopenaeus vannamei, but there were no reports on the SNP molecular markers of genes related to the traits of resistance to low dissolved oxygen in Litopenaeus vannamei

Method used

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  • A SNP marker associated with low dissolved oxygen tolerance of Litopenaeus vannamei and its screening and application
  • A SNP marker associated with low dissolved oxygen tolerance of Litopenaeus vannamei and its screening and application
  • A SNP marker associated with low dissolved oxygen tolerance of Litopenaeus vannamei and its screening and application

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Embodiment 1

[0029] In this example, through sequencing and BLAST comparison of the partial sequence of the superoxide dismutase SOD gene of Litopenaeus vannamei, one SNP site was screened out, which was located at the 118th base of the SOD gene of Litopenaeus vannamei. C / T mutation. The SNP molecular marker can be used to detect the polymorphism of the site in Litopenaeus vannamei population by direct sequencing, and analyze the correlation between the site genotype frequency and the low dissolved oxygen tolerance of Litopenaeus vannamei.

[0030] The correlation analysis between the SNP site 118C>T in the SOD gene of Litopenaeus vannamei and the tolerance to low dissolved oxygen was carried out according to the following steps:

[0031] (A) Grouping of experimental samples;

[0032] (B) Genomic DNA extraction of Litopenaeus vannamei: the conventional phenol-chloroform method was chosen for this implementation;

[0033] (C) Screening of the SNP site of the SOD gene of Litopenaeus vannam...

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Abstract

The invention relates to an SNP marker related with low dissolved oxygen survivability of litopenaeus vannamei, screening and applications thereof, and belongs to the technical field of molecular marker assisted selection of aquatic animals. An SNP molecular marker is cloned from SOD gene. The SNP marker is that the R in the 118-th base from the 5' terminal of the nucleotide sequence represented by SEQ ID No.1 is C or T. The SNP marker is prominently related with the character of tolerating low dissolved oxygen of litopenaeus vannamei, and thus can be applied to the molecular marker assisted selection so as to screen litopenaeus vannamei with strong performance of tolerating low dissolved oxygen. The provided SNP marker can be used to carry out early stage screening according to the actual needs, thus the efficiency and accuracy of breeding are effectively improved, the genetic level of litopenaeus vannamei is improved, species with strong stress resistance can be accurately and efficiently bred, the SNP marker is practical and the application range is wide.

Description

technical field [0001] The invention relates to a SNP marker related to the low dissolved oxygen tolerance of Litopenaeus vannamei and its screening and application, and belongs to the technical field of aquatic animal genetics and molecular marker-assisted selection breeding. Background technique [0002] Litopenaeus vannamei ( Litopenaeus vannamei ), also known as Penaeus vannamei, belongs to the phylum Arthropoda, Crustacea, Decapod, Penaeidae, Litopenaeus genus, and is a wide-temperature and wide-halt tropical shrimp. Litopenaeus vannamei has become one of the most important prawn species in my country due to its excellent characteristics such as fast growth, strong disease resistance, strong stress resistance, and adaptability to high-density farming. In recent years, the high-density culture model of Litopenaeus vannamei has been widely promoted, but in the process of high-density culture of prawns, due to the seasonal temperature and salinity changes in the culture w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 刘建勇陈晓敏吴勇
Owner GUANGDONG OCEAN UNIVERSITY
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