A SNP marker associated with low dissolved oxygen tolerance of Litopenaeus vannamei and its screening and application

A vannamene, low dissolved oxygen technology, applied in the direction of recombinant DNA technology, biochemical equipment and methods, microbial measurement/inspection, etc., can solve unseen problems, achieve strong practicability, improve efficiency and accuracy, The effect of increasing the genetic level

Inactive Publication Date: 2018-11-16
GUANGDONG OCEAN UNIVERSITY
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Problems solved by technology

However, there is no report on the SNP molecular markers related to the low-dissolved oxygen tolerance of Litopenaeus vannamei

Method used

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  • A SNP marker associated with low dissolved oxygen tolerance of Litopenaeus vannamei and its screening and application
  • A SNP marker associated with low dissolved oxygen tolerance of Litopenaeus vannamei and its screening and application

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Embodiment

[0028] Example: In this example, through sequencing and Clustal comparison of the partial sequence of Litopenaeus vannamei catalase (CAT) gene, one SNP site was screened, and the 155th SNP site screened was AG type vannamei, its tolerance to low dissolved oxygen was significantly higher than that of GG genotype individuals. The polymorphism of the SNP site can be detected in the population of Litopenaeus vannamei by direct sequencing method, and the correlation between the genotype frequency of the site and the low dissolved oxygen tolerance of Litopenaeus vannamei can be analyzed. In the process of selective breeding for stress resistance traits of Litopenaeus vannamei, AG-type individuals can be preferentially selected as breeding parents for this SNP locus, so as to improve the efficiency of selection.

[0029] The correlation analysis between the SNP site 155A>G in the catalase gene of Litopenaeus vannamei and the tolerance to low dissolved oxygen was carried out according...

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Abstract

The invention discloses an SNP marker relevant to the low dissolved oxygen tolerance of Litopenaeus vannamei as well as a screening method and an application of the SNP marker. The SNP marker is a basic group A or G on the 155th position counted from the 5' end of a nucleotide sequence as shown in SEQ ID NO.1; and the marker is tightly relevant to the low dissolved oxygen tolerance of the Litopenaeus vannamei and can be effectively applied to molecular marker assisted breeding of the Litopenaeus vannamei. The invention also discloses a primer pair for detecting or screening the SNP marker. Due to the adoption of the technical scheme, breeding materials of the Litopenaeus vannamei can be early selected according to actual breeding demands, so that the breeding efficiency and accuracy can be increased effectively, and furthermore the Litopenaeus vannamei variety with high stress resistance can be accurately and efficiently bred. The method disclosed by the invention is high in practicability and wide in applicability.

Description

technical field [0001] The invention relates to a SNP marker related to the low dissolved oxygen tolerance of Litopenaeus vannamei and its screening and application, and belongs to the technical field of aquatic animal genetics and molecular marker-assisted selection breeding. Background technique [0002] Litopenaeus vannamei ( Litopenaeus vannamei ), also known as Penaeus vannamei, belongs to the phylum Arthropoda, Crustacea, Decapod order, and is a wide-temperature and wide-halty tropical shrimp. Because of its fast growth rate, strong stress resistance, adaptability to high-density farming, and long breeding period, it has become the most important shrimp farming species in my country. At present, the culture of Litopenaeus vannamei in my country is mainly based on the traditional culture mode. However, as the breeding density continues to increase, traditional aquaculture is prone to hypoxia in the later stage of aquaculture, which affects the growth, feeding and molt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 刘建勇陈晓敏傅学丽
Owner GUANGDONG OCEAN UNIVERSITY
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