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A kind of SNP site related to the growth characteristics of yellow catfish and its detection and application

A yellow catfish and locus technology is applied in the field of molecular breeding genetic markers to achieve the effect of improving efficiency and accuracy

Active Publication Date: 2019-06-25
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of biotechnology, there are more and more methods for fish genetics and breeding technology. Molecular markers mainly refer to gene fragments that can reflect certain differences in genomes of individual organisms or groups, with high polymorphism and distribution. It has a series of advantages such as wide range and co-dominance, and has a wide range of applications in fish genetic breeding, genetic linkage map construction, kinship identification, germplasm resource research, etc.; the use of molecular markers to breed high-yield varieties with excellent growth traits is an important way to improve economic benefits. It is one of the ways of traditional Chinese medicine. Obtaining the genes and gene loci related to the growth characteristics of yellow catfish can assist the breeding of high-yield yellow catfish. There is no report on the genes and gene loci related to the growth characteristics of yellow catfish

Method used

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  • A kind of SNP site related to the growth characteristics of yellow catfish and its detection and application
  • A kind of SNP site related to the growth characteristics of yellow catfish and its detection and application
  • A kind of SNP site related to the growth characteristics of yellow catfish and its detection and application

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Experimental program
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Effect test

Embodiment 1

[0024] Example 1 SNP site screening analysis of GH gene

[0025] 1. Primer design and synthesis

[0026] Two pairs of primers were designed based on the cloned full-length sequence of the growth hormone gene of the peliotus chinensis to amplify the exon 4, intron 4, exon 5 regions of the GH gene and the 3'UTR region of the GH gene, respectively. See Table 1 for primer information.

[0027]

[0028] 2. Screening of SNPs

[0029] (1) Construction of DNA pool

[0030] Select 10 high and low body weight samples of yellow catfish in the test, measure the genomic DNA concentration of each sample with an ultraviolet spectrophotometer, and then dilute each sample to 100ng / ul, and take 10ul of equal concentrations and mix them to form a variety DNA pool for screening of SNPs sites.

[0031] (2) Mixed pool reaction system, as shown in Table 2

[0032]

[0033] PCR reaction program for the above reaction system: (i) pre-denaturation at 94 °C for 5 min; (ii) denaturation at 94 ...

Embodiment 2

[0043] Example 2 Analysis of genetic characteristics of SNP sites

[0044] See Table 5 for the analysis of the population genetic characteristics of the five SNP polymorphic sites in the study. Among them, the number of alleles of 5 loci is 2, the effective allele number of locus 1674G>A is the highest 1.8989, and the effective number of alleles of locus 2285T>C is the lowest 1.3473; loci 2154T>G and 2285T> C is low polymorphism, loci 1674G>A, 2100A>G and 2822A>G are moderate polymorphisms.

[0045]

Embodiment 3

[0046] Example 3 Association analysis of SNPs polymorphisms and growth traits

[0047] Using the general linear model (GLM) in SAS software, the genotype of the SNP site and the main growth traits of the yellow catfish (weight, total length, body length, body height, body thickness, head length, tail peduncle length, tail peduncle height) Correlation analysis was carried out, and sites with significant differences were tested by variance analysis, and Duncan method was used for multiple comparisons. The results showed that the genotypes of 1674G>A and 2154T>G had no significant difference with each growth trait (Table 6 and Table 8); the body thickness of genotype GG in locus 2100A>G was significantly thicker than that of genotype AA (PC was significantly longer than that of genotype CT (PG was significantly longer than that of genotype AG (P<0.05), and the body length of genotype GG was significantly longer than that of genotype AA (P<0.05), and Very significantly longer tha...

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Abstract

The invention discloses a pelteobagrus fulvidraco growth characteristic related SNP locus, which can be at the 2100th position from the 5' end of a GH gene shown as SEQ ID NO:1, and has a basic group of A or G, or can be at the 2285th position from the 5' end of the GH gene shown as SEQ ID NO:1, and has a basic group of T or C, or can be at the 2822nd position from the 5' end of the GH gene shown as SEQ ID NO:1, and has a basic group of A or G. The invention also screens out a primer for specific detection of the SNP locus. The SNP locus provided by the invention is closely related to the full-length character of pelteobagrus fulvidraco, can be effectively used for molecular mark assisted breeding of pelteobagrus fulvidraco, and then the growth rate is selected according to actual breeding needs to conduct early selection on a pelteobagrus fulvidraco breeding material, thus further improving the breeding efficiency and accuracy effectively.

Description

technical field [0001] The invention relates to the technical field of genetic markers in molecular breeding, and more specifically, relates to a SNP site related to the growth characteristics of yellow catfish and its detection and application. Background technique [0002] Yellow catfish is a small freshwater economic fish widely cultivated in my country. It is popular among consumers because of its tender meat, rich nutrition, and high meat content. The breeding parents of yellow catfish are mainly wild-caught or cultivated populations for several generations. Artificial breeding, its growth rate, disease resistance and breeding performance are weak, so it is of practical significance to carry out two kinds of breeding work of yellow catfish. [0003] At present, many scholars have carried out a large amount of research on the genetic breeding of yellow catfish, and obtained many achievements. With the development of biotechnology, there are more and more methods for fish...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 刘文生李美娟朱雯璐王娟廖良源
Owner SOUTH CHINA AGRI UNIV
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