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MnRXR gene of macrobrachium nipponensis, and amplification primer group and amplification method thereof

A primer set, Macrobrachium technology, applied in the field of Macrobrachium japonica MnRXR gene

Inactive Publication Date: 2013-03-27
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

[0004]Currently, several shrimp and crab RXR genes have been cloned, including: Marsupenaeus japonicas, Fenneropenaeus chinensis , Crangon crangon, etc., but the RXR gene of Macrobrachium japonicus (named here: MnRXR) has not been reported

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  • MnRXR gene of macrobrachium nipponensis, and amplification primer group and amplification method thereof
  • MnRXR gene of macrobrachium nipponensis, and amplification primer group and amplification method thereof
  • MnRXR gene of macrobrachium nipponensis, and amplification primer group and amplification method thereof

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Embodiment Construction

[0026] Below in conjunction with embodiment, further set forth the present invention.

[0027] Macrobrachium japonicus in the present invention is provided by fishermen in Wuxi Taihu Lake, reagents can be purchased from the market, reagents such as RNA purification are purchased from Promega Company of the United States, etc., 3'RACE and 5'RACE kits are purchased from Takara Biology (Dalian) Co., Ltd. (Takara ).

[0028] Total RNA extraction: Take 100 mg Macrobrachium japonicus hepatopancreas tissue and grind it in a mortar with liquid nitrogen, and extract total RNA by Trizol method. Trizol reagent is from Invitrogen Company, and the extraction method is according to the instruction manual.

[0029] Japanese river prawn wxya Cloning of the full-length cDNA sequence of the gene, including two alternative splice forms MnRXR-L and MnRXR-S :

[0030] The published RXR-like protein sequence (GenBank accession number: ABF74729.1) of the crustacean Daphnia magna was used as th...

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Abstract

The invention discloses a MnRXR gene of macrobrachium nipponensis, and an amplification primer group and an amplification method thereof. A full-length cDAN sequence of the MnRXR gene is cloned from the hepatopancreas tissue of macrobrachium nipponensis. The gene comprises two variable spliceosomes: MnRXR-L and MnRXR-S. The invention lays an important base for research on functions of the MnRXR gene of macrobrachium nipponensis, in particularon important effects in a moult cycle.

Description

technical field [0001] The present invention relates to Macrobrachium japonicus wxya Gene, its amplification primer set and amplification method. Background technique [0002] Retinoid X receptor (RXR, retinoid x receptor) is one of the most conserved members of the nuclear receptor superfamily in animals, and is found in both vertebrates and invertebrates. RXR has important biological functions, and plays an important role in many biological processes such as vertebrate development, metabolism, cell differentiation, apoptosis, etc., and in invertebrates, it mainly interacts with ecdysteroid receptor (ECR) Forming a heterodimer, ecdysone enters the cell and combines with it to form a functional form, thereby regulating the expression of a series of downstream molting-related genes, and finally triggering the molting response of animals. The RXR-ECR heterodimer is an essential key node in the signaling pathway of molt molecules such as crustaceans. [0003] Japanese river ...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/11C12N15/10
Inventor 沈怀舜周鑫柏爱旭任秀芳
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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