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Application of arabidopsis thaliana MAPKKK kinase in breeding

A technology of Arabidopsis thaliana and kinase, which is applied in the application field of Arabidopsis MAPKKK kinase in breeding, can solve the problems that the influence needs to be further studied, and achieve the effect of saving time and accurate seed selection

Active Publication Date: 2019-03-19
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] In view of the advantages of Arabidopsis in genetic manipulation, it is widely used in the study of various processes of plant life activities, and a series of important discoveries have been made; but for the study of Arabidopsis protein kinase and its effect on the growth of Arabidopsis The impact still needs to be further studied and applied to the cultivation of Arabidopsis to provide a theoretical basis for future breeding work

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  • Application of arabidopsis thaliana MAPKKK kinase in breeding
  • Application of arabidopsis thaliana MAPKKK kinase in breeding
  • Application of arabidopsis thaliana MAPKKK kinase in breeding

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Embodiment 1

[0021] This embodiment 1 provides a kind of application of Arabidopsis thaliana MAPKKK kinase in breeding; wherein, Arabidopsis thaliana 47 gene is MAPKKK kinase, and its two alternative splicing forms have two phenotypes of drought resistance and early flowering respectively; There are four spliced ​​forms of 47 genes (such as figure 1 shown), named AS1, AS2, AS3 and AS4; the nucleotide sequence of AS1 is SEQ ID NO.1, the nucleotide sequence of AS2 is SEQ ID NO.2, and the nucleotide sequence of AS3 is SEQ ID NO .3, the nucleotide sequence of AS4 is SEQ ID NO.4; the amino acid sequence of the protein encoded by AS1 is SEQ ID NO.5, the amino acid sequence of the protein encoded by AS2 and AS4 is SEQ ID NO.6, the amino acid sequence of the protein encoded by AS3 The sequence is SEQ ID NO.6, and its three proteins are located in the nucleus and cytoplasm through GFP; the drought resistance phenotype experiment shows as follows: image 3 As shown, the 47-gene full deletion mutant...

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Abstract

The invention discloses an application of arabidopsis thaliana MAPKKK kinase in breeding, and relates to the technical field of agricultural breeding. The breeding includes creation of the drought resistant idioplasm and early blossoming idioplasm of crucifer by a transgenic technology. The crucifer includes oilseed rape and broccoli. The MAPKKK kinase has four spliceosomes, named as AS1, AS2, AS3and AD4; an overexpression plant AS1-OE of AS1 and a mutant CRISPPAS3-2 lost by AS3 both express the non-drought resistant phenotype; AS3-OE plant and a mutant CRISPRAS1-1 lost by AS1 both express the drought resistant phenotype; AS1-OE plant and AS2 / 4-OE plant express the early blossoming phenotype, and AS3-OE plant expresses the late blossoming phenotype. The research can provide theoretical basis for the crucifer transgene breeding operation.

Description

technical field [0001] The invention relates to the technical field of agricultural breeding, in particular to the application of Arabidopsis MAPKKK kinase in breeding. Background technique [0002] Arabidopsis belongs to Brassicaceae, Angiosperm Phylum, Dicotyledon Class. Biennial herb, 7-40 cm tall. Basal leaves are rosette-shaped with petioles, obovate or spoon-shaped leaves; cauline leaves are sessile, lanceolate or linear. Raceme terminal, with 4 petals, white. The advantages of Arabidopsis are small plants, short generation time, many seeds, and strong vitality. The Arabidopsis genome is 1 / 80 of the currently known plant genome, which makes it relatively easy to clone its related genes. [0003] Arabidopsis research includes forward genetics and reverse genetics. Forward genetics follows a mindset from mutant phenotype analysis to gene function understanding, focusing first on mutants with certain defects. For example, if you want to study the gene regulation pro...

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Application Information

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IPC IPC(8): C12N15/54C12N9/12C12N15/82A01H5/00A01H6/20
CPCC12N9/12C12N15/827C12N15/8273C12Y207/11025
Inventor 刘鹰高陈沫先武茹茹朱福远
Owner SHANDONG AGRICULTURAL UNIVERSITY
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