Extraction method of total flavone of Hovenia dulcisThunb

An extraction method and technology of total flavonoids are applied in the directions of pharmaceutical formulations, preparations for skin care, medical preparations containing active ingredients, etc., and can solve the problems of restricting the utilization and efficacy of total flavonoids of Aurantium chinensis, and low purity.

Inactive Publication Date: 2009-01-07
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the solvent extraction method of the total flavonoids of Hovenia dulcis Fructus has been reported in the literature, but it only simply extracts the total flavonoids, and does not combine with other methods to separate and purify the crude extract of total flavonoids. Therefore, the total flavonoids content in the extract are all below 11%, and the purity is very low; there are also reports on the preparation method of monomeric flavonoids in Hovenia dulcis fruit, but it only extracts an active ingredient of flavonoids, and does not involve the preparation method of total flavonoids; and other active parts of Hovenia dulcis There are no literature reports on the preparation methods of flavonoids such as leaves, flowers, roots, stems, branches, skins and pomace, which severely limit the utilization of total flavonoids of Hovenia dulcis and the exertion of medicinal effects

Method used

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  • Extraction method of total flavone of Hovenia dulcisThunb
  • Extraction method of total flavone of Hovenia dulcisThunb
  • Extraction method of total flavone of Hovenia dulcisThunb

Examples

Experimental program
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Effect test

Embodiment 1

[0055] Example 1: Extraction of Hovenia dulcis total flavonoids

[0056] Take Hovenia dulcis leaf 1Kg and pulverize to 50 meshes, add 75% ethanol solution (V / V) according to the solid-liquid ratio of 1:50 (g / mL), continuously heat and reflux for extraction for 9 hours, recover the solvent to remove ethanol, and adjust the concentration of the extract. pH=6, the processed macroporous resin LSD-958 on the filtrate was washed with 4 times of resin bed volume aqueous solution and 3 times of resin bed volume 20% ethanol solution (V / V) to remove impurities, and the washing solution was discarded and used The 95% ethanol solution (V / V) of 7 times the volume of the resin bed was eluted, the eluate was collected, concentrated to a thick paste and dried to obtain the total flavonoids of Hovenia dulcis. The total flavonoid content of Hovenia dulcis was determined to be 66%, of which quercetin, kaempferol, myricetin, (+)-dihydromyricetin, hovenia dulcis alcohol, (-)-catechin, hovenia dulc...

Embodiment 2

[0057] Example 2: Extraction of Hovenia dulcis total flavonoids

[0058] Take 1Kg of mature and dried Hovenia dulcis and crush it to 60 mesh, add distilled water and soak for 40 minutes at a solid-liquid ratio of 1:40 (g / mL), control the microwave power to 900W, irradiate for 50 seconds, take it out, cool it to room temperature quickly, and irradiate again For 20 seconds, repeat the same operation 3 times, filter, and combine the extracts. The extract was concentrated to 20% (V / V) under reduced pressure, suspended in water, then extracted with n-hexane and ethyl acetate in sequence, the solvent was recovered and the organic solvent extract was retained. Take the ethyl acetate extract and dissolve it with ethanol, add diatomaceous earth according to 30% by weight, stir at room temperature for 30 minutes, filter and wash with ethanol, combine the filtrates, concentrate and dry in vacuo to obtain total flavonoids of Hovenia dulcis. The content of the total flavonoids was determi...

Embodiment 3

[0059] Example 3: Extraction of Hovenia dulcis total flavonoids

[0060] Get 1Kg of dried pomace after removing the juice from Hovenia dulcis fruit stalk, pulverize, pass through a 100-mesh sieve, filter out impurities such as cellulose, and dry the powder, 8 times the amount (g / mL) of 70% ethanol solution (V / V) 80 Heat reflux extraction at ℃ for 3 times, 2 hours each time, combine the filtrates, concentrate in vacuo to remove ethanol, dissolve the extract in 3 times distilled water by weight, put it on a polyamide column, the adsorption flow rate is 5BV / h, and the polyamide column diameter to height ratio is 1 : 8, the loading amount is 400mg / mL (based on the amount of crude drug), first eluted with water and the outflow is nearly colorless, then rinsed with 4 times the resin bed volume 30% ethanol solution (V / V) to remove impurities, and the rinse solution was discarded , eluted with 95% ethanol solution (V / V) of 5 times the polyamide bed volume, the elution flow rate was 4B...

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Abstract

The invention discloses a method for extracting total flavonoids of hovenia dulcis. The method adopts the solvent extraction method and one optional method of the solvent extraction method, the macro-porous resin adsorption method, the supercritical fluid extraction method, the column chromatography or liquid-liquid countercurrent distribution chromatography, or the free combination of the methods. The total flavonoids of hovenia dulcis is extracted from any one part of hovenia acerba, such as fruit stems, fruits, seeds, seed-cases, leaves, flowers, roots, stems, branches, skins and fruit residues, etc., or the combination thereof and is further separated and purified. The prepared total flavonoids is a combination containing two or more flavonoid active components, wherein the total flavonoids comprises the main active components as follows: quercetin, kaempferol, myricetin, (+)-dihydromyricetin derivates, etc.; the total percentage composition of various flavonoid components by weight is 12% to 100%; and the preparation method is applicable to pharmaceutical industry, food industry, cosmetic industry and health-care product industry.

Description

technical field [0001] The invention relates to an extraction method and a quality control method of total flavonoids of Hovenia dulcis and its application in the fields of medicine, food, cosmetics and health care products, belonging to the field of natural products. Background technique [0002] Hovenia dulcis Thunb (Hovenia dulcis Thunb) is a plant of the genus Hovenia dulcis Thunb, which is extremely rich in resources in my country. Its fruit or seeds with fleshy stalks are used as medicine, known as "Hovenia dulcis Thunb", which can relieve alcohol poisoning, quench thirst and relieve thirst. Annoyance, anti-vomiting, diuresis. Indications of drunkenness, polydipsia, vomiting, dyspepsia, rheumatic numbness, spasm of hands and feet. Hovenia dulcis leaves, flowers, roots, stems, branches, bark, and sap can sober up the nerves, promote urine excretion, accelerate intestinal peristalsis, dispel wind, dredge collaterals, relieve spasm, quench thirst and annoyance, supplement...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/72A61K8/97A61K9/48A61K9/20A61K9/16A61K9/10A61K9/06A61K9/14A23L1/29A23L2/38A61P1/16A61P31/04A61P39/06A61P25/32A61Q19/00A61K125/00A61K127/00A61K129/00A61K131/00A61K133/00A61K135/00A23L33/00
Inventor 张存莉兰光张丽刘元伟雷中锋王家龙杨质瑾
Owner NORTHWEST A & F UNIV
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