Method for preparing collagen oligopeptide

A technology of collagen and oligopeptides, which is applied in the field of preparation of collagen oligopeptides, and can solve problems such as deep yellow color and bad smell

Inactive Publication Date: 2012-07-11
胡如桂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The purpose of the present invention is to provide a preparation method of collagen oligopeptide, which solves the common problems of current collagen products, such as fishy smell, dark yellow color, etc., and the collagen oligopeptide prepared by the present invention With good absorption and permeability, it can be widely used in cosmetics, food and other industries

Method used

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  • Method for preparing collagen oligopeptide

Examples

Experimental program
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Effect test

Embodiment 1

[0056] A preparation method of collagen oligopeptides, comprising the following steps:

[0057] (1) Cut the fat-shaved, depilated and cleaned fresh pigskin into small pieces with a length and width of 1 cm, add water twice the weight of the pigskin, then add hydrochloric acid to adjust the pH value to 5, stir and soak at room temperature for 1 hour;

[0058] (2) Wash the pigskin obtained in step (1) to neutrality, add deionized water twice the weight of the pigskin, add sodium lauryl sulfate with a weight of 0.005% of the pigskin, stir and soak at 20°C for 20 hours ;

[0059] (3) Wash the pigskin obtained in step (2) to neutrality, drain the water, add deionized water until the mass fraction of the substrate is 10%, then add microbial protease with 0.5% pigskin weight for enzymolysis, and the enzyme The hydrolysis temperature was 30°C, and the enzymolysis time was 8 hours to obtain a hydrolyzate;

[0060] (4) adding 0.05% activated carbon of the weight of the hydrolyzed solu...

Embodiment 2

[0065] A preparation method of collagen oligopeptides, comprising the following steps:

[0066] (1) Cut the shaved, depilated and cleaned fresh donkey skin into small pieces with a length and width of 2 cm, add water 20 times the weight of the donkey skin, add hydrochloric acid to adjust the pH value to 0.2, stir and soak at room temperature for 10 hours;

[0067] (2) Wash the donkey skin obtained in step (1) until neutral, add deionized water 20 times the weight of donkey skin, add fatty alcohol polyoxyethylene ether with 2% weight of donkey skin, stir and soak at 50°C for 1 hour ;

[0068] (3) The donkey skin obtained in step (2) is cleaned to neutrality, drained, and deionized water is added to a mass fraction of the substrate of 50%, and then 10% of the donkey skin weight is added with microbial protease for enzymolysis. The hydrolysis temperature is 60°C, and the enzymolysis time is 1.5h to obtain a hydrolyzate;

[0069] (4) adding 10% activated carbon of the weight of ...

Embodiment 3

[0074] A preparation method of collagen oligopeptides, comprising the following steps:

[0075] (1) Cut the fat-shaved, depilated and cleaned fresh pigskin into small pieces with a length and width of 3 cm, add water 10 times the weight of the pigskin, then add hydrochloric acid to adjust the pH value to 3.5, stir and soak at room temperature for 5 hours;

[0076] (2) Wash the pigskin obtained in step (1) to neutrality, add deionized water 10 times the weight of the pigskin, add sodium lauryl sulfate with 1% weight of the pigskin, stir and soak at 35°C for 10 hours ;

[0077] (3) Clean the pigskin obtained in step (2) to neutrality, drain the water, add deionized water until the mass fraction of the substrate is 30%, then add microbial protease with 5% pigskin weight for enzymolysis, and the enzyme The hydrolysis temperature was 50°C, and the enzymolysis time was 4 hours to obtain a hydrolyzate;

[0078] (4) adding 5% activated carbon of the weight of the hydrolyzed solution...

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Abstract

The invention discloses a method for preparing collagen oligopeptide. Animal skins are taken as raw materials, and are subjected to acid and surfactant pretreatment, enzymolysis, decoloration, membrane separation and drying to form the collagen oligopeptide. The relative molecular weight of the prepared collagen oligopeptide is mainly distributed below 1,000Dalton, and preferably, the Gaussian distribution peak area percentage of oligopeptide with the relative molecular weight of less than 1,000Dalton is over 85 percent and preferably over 90 percent. The method solves the general problems that the conventional collagen products have odor, are dark yellow and the like; and the obtained collagen oligopeptide powder is white, free of odor and good in mouthfeel, has high absorptivity and penetrability and can be widely applied to the cosmetics industry, the food industry and the like.

Description

technical field [0001] The present invention relates to a preparation method of oligopeptides, in particular, the present invention relates to a preparation method of collagen oligopeptides. Background technique [0002] Collagen is a biological polymer substance, the English scientific name Collagen. Playing the role of binding tissue in animal cells, it is one of the most critical raw materials in the biotechnology industry, and it is also the best biomedical material with a huge demand. Its application fields include biomedical materials, cosmetics, food industry, etc. [0003] Collagen is a nutrient that must be supplemented by the human body to delay aging. It accounts for more than 30% of the total protein in the human body. There are about 3 kg of collagen in the body of an adult. It widely exists in the skin, bones, muscles, cartilage, joints, and hair tissues of the human body, and plays a triple anti-aging role of support, repair, and protection. [0004] Howeve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K2/00
Inventor 胡如桂于雪源
Owner 胡如桂
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