Sporidiobolus pararoseus bacterial strain and application thereof

A yeast strain and lock-throwing technology, applied in fermentation, fungi and other directions, can solve the problems of short fermentation cycle, simple and extensive nutritional requirements, and easy to decline in traits.

Active Publication Date: 2011-07-06
JIANGNAN UNIV
View PDF1 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the conjugation of B. trispora, its traits are easy to decline, and there are also disadvantages such as complicated fermentation technology, long fermentation cycle, and high cost.
Fermentation of β-carotene by Rhodotorula has the advantages of simple and extensive nutritional requirements, short fermentation period, simple reproductive mode, low nutritional requirements and high-density cultivation, but the current fermentation level is not as good as that of B. trispora. Capable of industrial application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sporidiobolus pararoseus bacterial strain and application thereof
  • Sporidiobolus pararoseus bacterial strain and application thereof
  • Sporidiobolus pararoseus bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Determination of the strain culture of Sporidiobolus pararoseus JD-2 and the fermentation process for enriching β-carotene.

[0043] The selected medium is as follows:

[0044] (1) Incline activation medium (g / L): glucose 20, peptone 1, yeast extract 1, pH 6.0.

[0045] (2) Liquid seed medium (g / L) enriched in β-carotene: glucose 40, corn steep liquor 20, (NH4) 2 SO 4 5. KH 2 PO 4 1. MgSO 4 ·7H 2 O 0.5, pH 6.0, 100mL / 500mL Erlenmeyer flask.

[0046] (3) Liquid fermentation medium enriched with β-carotene (g / L): glucose 40, corn steep liquor 20, (NH4) 2 8O 4 5. KH 2 PO 4 1. MgSO 4 ·7H 2 O 0.5, pH 6.0.

[0047] Fermentation culture method:

[0048] (1) Slant Activation Culture: The strain was transferred from the preserved slant to the activated slant medium, and cultured at 28° C. for 48 hours.

[0049] Liquid seed culture: the strains were inserted from the activated slant medium into the liquid seed medium, and cultured at 28° C. for 16 hou...

Embodiment 2

[0052] Example 2 The strain culture of Sporidiobolus pararoseus JD-2 and the determination of the fermentation process for enriching rhododromolene.

[0053] The selected medium is as follows:

[0054] (1) Incline activation medium (g / L): glucose 20, peptone 1, yeast extract 1, pH 6.0.

[0055] (2) Liquid seed medium (g / L) enriched with rhodozymene: glucose 40, yeast extract 20, (NH4) 2 SO 4 5. KH 2 PO 4 1. MgSO 4 ·7H 2 O 0.5, pH6.0, 100mL / 500mL Erlenmeyer flask.

[0056] (3) Liquid fermentation medium (g / L) enriched with rhododactylene: glucose 40, yeast extract 20, (NH4) 2 SO 4 5. KH 2 PO 4 1. MgSO 4 ·7H 2 O 0.5, pH 6.0.

[0057] Fermentation culture method:

[0058] (1) Slant Activation Culture: The strain was transferred from the preserved slant to the activated slant medium, and cultured at 28° C. for 48 hours.

[0059] Liquid seed culture: the strain was transferred from the activated slant medium into the liquid seed medium, and cultured at 28° C. for...

Embodiment 3

[0062] Example 3 Extraction of Carotenoids

[0063] The specific process for preparing the carotenoid extract in the aforementioned Examples 1 and 2 can be: centrifuge the bacterial solution containing Sporidiobolus pararoseus JD-2 at 4000r / min for 10min to obtain the bacterial cells, wash with deionized water Once, after centrifuging to remove water, add 0.6mol / L hydrochloric acid, raise the temperature in the sterilizing pot to 115°C, quickly deflate and reduce the pressure, centrifuge to remove hydrochloric acid, wash the precipitate with deionized water once, centrifuge again to remove water, add volume The mixture of alcohol and n-hexane with a ratio of 4:1 was shaken and extracted at room temperature for 30 minutes, then centrifuged (4000r / min, 10 minutes), and the supernatant was concentrated at 40°C and a vacuum of 0.1Mpa to obtain Carotene crude extract.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a sporidiobolus pararoseus bacterial strain and application thereof. The bacterial strain is a sporidiobolus pararoseus bacterial strain JD-2 CCTCC M 2010326. A process of producing carotene by using the sporidiobolus pararoseus bacterial strain JD-2 comprises the following steps of: (1) activating and culturing the sporidiobolus pararoseus bacterial strain; (2) fermenting and culturing the activated strain obtained in the step (1) at the pH value between 5 and 9; and (3) extracting the bacterial liquid obtained in the step (1) and / or the step (2) to obtain a carotene extract. The yield of the carotene produced by using the sporidiobolus pararoseus bacterial strain is generally up to 500-700 mug / g, process conditions such as temperature, pH value and the like are wide, the needed carotene is selectively gathered by controlling different fermentation conditions, and the produced carotene belongs to a pure natural product, does not contain any artificial color or other chemical additives, and can be widely applied to the fields of food industry, cosmetic industry, pharmaceutical industry and the like.

Description

technical field [0001] The invention relates to a yeast in the field of microorganisms and microbial fermentation and its application, in particular to a lock-throwing yeast strain and its application in a carotenoid production process. Background technique [0002] Carotenoids are a class of fat-soluble yellow-red pigments widely present in plants, animals and microorganisms, with the structure C 40 The structure of polyisoprene, in which the difference in the conjugated double bond can cause the color change. Carotenoids are mainly divided into carotene and carotene (lutein), and carotene includes: α-carotene, β-carotene, γ-carotene, lycopene and rhodophyllene ( Torulene); carrot alcohols (lutein) include: zeaxanthin, cryptoxanthin, lutein, capsanthin, gardenia yellow. [0003] Beta-carotene has the highest vitamin A activity of all carotenoids. It can effectively protect various biomacromolecules in cells from free radical damage, thereby maintaining normal life metabo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P23/00
Inventor 张伟国钱和韩梅
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap