32 results about "Collagenase activity" patented technology

The invention relates to a bacillus subtilis Col04 bacterial strain with a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.4500. The bacillus subtilis Col04 bacterial strain has the advantages of simple nutrition requirements, quick growth, and high collagenase producing activity. According to the researches, the collagenase produced by the bacillus subtilis Col04 bacterial strain is insensitive to most metal ions, extensive in pH range, high in collagenase activity and stronger in tolerable temperature capability in comparison with the conventional collagenase from other bacterial strains. Moreover, the bacterial strain is safe and non-toxic, and therefore, the bacterial strain has extensive economic prospect in degrading collagenase or gelatin and being used in food and cosmetics industries.

A composition comprising cartilage extract, grape seed extract and tomato extract was found to have remarkable anti-oxidant effect and free-radical inhibition. The tomato extract comprises lycopene. The composition which comprises a hydrophilic antioxidant, a lipophilic (hydrophobic) antioxidant, and a cartilage extract dramatically increases collagen synthesis in the dermis. Furthermore, the composition lowers collagenase activity and levels of advanced glycation end products (AGE). The signs of ageing, such as photoageing due to exposure to UV radiation, are related to the levels of collagen syntheses and free-radical oxidation. Compositions of the invention are intended for the treatment of ageing skin and the delaying of the onset of the signs of ageing in healthy skin.

The present invention relates to a composition for improving skin conditions comprising matrine and oxymatrine as an active ingredient. Matrine and oxymatrine have lower cytotoxicity than retinol used as anti-wrinkle agents and exhibit the inhibition effect on collagenase activity and promotion effect on collagen biosynthesis at a molecular level, contributing to excellent efficacy in improvement of skin wrinkles. In addition, both matrine and oxymatrine exhibit the inhibition effect on melanin production by inhibiting intracellular tyrosinase activity, the improving effects of UV-induced skin damage and the skin growth promotion or hair loss prevention. Therefore, matrine and oxymatrine have the excellent improvement effects on skin conditions. Furthermore, matrine and oxymatrine have the excellent anti-obesity and anti-oxidation effects. The composition of this invention can be applied to cosmetic, pharmaceutical and food composition having no cytotoxicities and side effects.

The present invention discloses catalytic rate improving keratinase mutants and a preparation method thereof, and belongs to the field of enzyme engineering. According to the present invention, the C terminal structure of keratinase KerSMD is sheared to obtain a series of keratinase mutants, wherein the catalytic rate of the keratinase mutants on the casein substrate is significantly improved and is increased by 33-103%, particularly the mutant V355 has effects of significantly improved catalytic rate, significantly improved enzyme activity, enhanced salt tolerance and enhanced detergent resistance, the characteristics of no collagenase activity and salt tolerance of the V355 can be used in the leather processing industry, the catalytic rate on the substrate is rapid, and the V355 has the characteristic of detergent resistance so as to be subjected to laundry additive production application.

The invention relates to the technical field of cell separation, in particular to adipose tissue digestive juice and a method for rapidly obtaining an SVF (stromal vascular fraction) cell. The adiposetissue digestive juice comprises the following ingredients: I-type collagenase, HEPES buffer solution and a DEMEM/F12 culture medium. According to the adipose tissue digestive juice provided by the invention, the pH value of a digestive system can be stably maintained between 6.8-8.2, so that the activity of the I-type collagenase is optimal. Therefore, complete digestion can be realized in a short time. The time for obtaining the SVF according to the traditional digestive scheme is 40 min, while the SVF with high quality and high quantity can be obtained within 15-20 min in such a way that the digestive juice is adopted for digesting adipose tissues.

The invention relates to a collagenase producing strain, namely bacillus cereus, heterologous expression of a collagenase gene thereof and application thereof. The strain is separated and sieved from a soil sample and belongs to bacillus cereus after being identified. The strain is capable of extracellularly producing protease with collagen hydrolytic activity, and the protease with a molecular weight of 100kDa and collagenase activity is obtained by separating and purifying thallus fermentation supernate. Meanwhile, the collagenase gene of the strain is obtained through gene mining, and a collagenase protein with a molecular weight of 100kDa is obtained through heterologous expression and purification of escherichia coli. Researches confirm that the collagenase obtained through fermentation and purification of the Col15 strain and the collagenase obtained through heterologous expression and purification of escherichia coli are the same protein containing signal peptide. The collagenase produced by bacillus cereus Col15 has the properties of relatively wide temperature tolerance and pH value tolerance range and the like. Products obtained by hydrolyzing collagen and analogues thereof by virtue of the strain or the collagenase have wide economic prospects in the industries of foods, cosmetics, medical treatment and the like.

The purpose of the present invention is to acquire a highly proliferative cell at a high efficiency from a sample derived from a biological tissue. Provided is a composition for dispersing a biological tissue, wherein a solution formulation of the composition has a collagenase activity of 0.30-10 U/mL, said collagenase activity being determined by a method for measuring FALGPA-decomposing activity, and a trypsin activity of 0-30 U/mL at a formulation concentration of the composition, said trypsin activity being determined by a method for measuring BAEE hydrolytic activity.

The invention relates to nutritional oil for preventing a hand and a foot from chapping, which is mixed with transparent hyaluronic acid, honey, olive oil, egg white, cassia twigs, honeysuckle flowers, safflower, angelica dahurica and ionized water by a certain ratio, wherein each 100g of nutritional oil components comprise 25 to 35ml of transparent hyaluronic acid, 15 to 25ml of honey, 10 to 15ml of olive oil, 10 to 15ml of egg white, 2 to 4g of cassia twigs, 1 to 3g of honeysuckle flowers, 2 to 4g of safflower, 3 to 5g of angelica dahurica and the balance ionized water. The nutritional oil can be directly applied to a hand or a foot, or is applied on mask paper and then on the hand chap or foot chap, can soften horniess, moisturize and supplement collagen, promote the activity of skin polymerase and collagenase, and quickly promote wound healing. After being used often, the nutritional oil for preventing the hand and the foot from chapping can prevent skin chap and dryness, has the functions of moisturizing and caring skin, and can keep the the hand skine and foot skin wet, fine and smooth.

The invention discloses a diol-type ginsenoside nanoemulsion and a preparation method thereof and application thereof. The diol-type ginsenoside nanoemulsion comprises the following components: an aqueous phase, an oil phase and a mixed emulsifier composed of a diol-type ginsenoside extract and water; Wherein the mass ratio of the water phase to the oil phase is 5 to 8: 45; that mas ratio of the oil phase to the mixed emulsify is 7. 5-8: 2-2.5; the mass concentration of the diol-type ginsenoside extract in the water phase is 15-25%; and the content of the diol-type ginsenoside Rb1, Rb2, Rc andRd contained in the diol-type ginsenoside extract is not less than 38%. Ginsenoside nanoemulsion prepared by the method has good skin permeability and skin retention, and can promote collagen synthesis and inhibit collagenase activity, so as to achieve the function of anti-aging skin.

The present invention provides a crude extract from the bark of an Acer rubrum (AR) tree and/or Picea mariana (PM), method of preparation and its use for inhibiting the signs of skin aging (such as wrinkles and/or dehydration) caused by at least one of: increased elastase activity, increased collagenase activity, decreased elastin synthesis, decreased collagen synthesis or decreased involucrin synthesis, in skin fibroblast cells, particularly those of a human subject.

The invention provides a collagenase inhibitor, eye essence containing the collagenase inhibitor and a preparation method of the eye essence. The collagenase inhibitor includes a rhodiola crenulata extract and a chamomile extract; and based on the total mass of the collagenase inhibitor, the addition of the rhodiola crenulata extract is 3-30%, and the addition of the chamomile extract is 70-97%. The collagenase inhibitor has excellent inhibition effects on the activity of collagenase, and has no side effects on human bodies.

The invention discloses an anti-enzymolysis collagen compound and application thereof, the anti-enzymolysis collagen compound is composed of polymer microspheres carrying one or more collagenase inhibitors and collagen, the mass ratio of the polymer microspheres to the collagen is 1: 20-20: 1, and the anti-enzymolysis collagen compound can be cross-linked as required. The collagenase inhibitor is carried and slowly released through the polymer microspheres, so that the collagenase activity in a local environment is reduced, the decomposition rate of a collagen product in tissues is reduced, the action time of the collagen product is prolonged, the replacement and use frequency of the collagen product can be reduced, and the use cost of the collagen product is reduced.

The present invention relates to a cosmetic, pharmaceutical, and food compositions comprising veratric acid or acceptable salt thereof as an active ingredient. The composition of the present invention has an excellent effect of reducing skin wrinkles through molecular mechanisms including the promotion of collagen synthesis and inhibition of collagenase activity. Also, the composition of the present invention has an excellent effect of reducing wrinkles by absorbing UVB to prevent UV rays from penetrating the skin, promotion of collagen synthesis and inhibition of collagenase activity. In addition, the composition of the present invention has effect of promotion of hair growth and the prevention of hair loss through growth promotion of hair dermal papilla cells, stimulation of IGF-1 secretion, and inhibition of TGF-beta1 secretion. Also, because the composition of the invention is derived from plants such as natural materials, it is harmless to the human body and can be safely applied to cosmetic, pharmaceutical, and food compositions.

The invention provides a collagenase inhibitor, an anti-aging skin-brightening toner containing the collagenase inhibitor and preparation methods. The collagenase inhibitor comprises a red pomegranate bark extract and a purslane herb extract, based on the total mass of the collagenase inhibitor, the addition amount of the red pomegranate bark extract is 26-74%, and the addition amount of the purslane herb extract is 26-74%. The collagenase inhibitor of the invention has an excellent inhibitory effect on the activity of collagenase, and has no side effects on the human body.

A method of measuring soluble or insoluble cell or tissue-associated collagenase activity. The substrate includes native (fibrillar) collagen fragments that were stained with Coomassie Brilliant Blue R-250. Incubation with collagenase can be observed in real-time by the generation of digested smaller fragments. The degraded blue fragments are obtained by filtration through class fibers, onto which intact collagen fibrils are retained. The filtrate containing the blue collagen fragments is incubated with a detergent in order to extract the blue dye, and the mixture is centrifuged in order to separate the dye in the supernatant from the pellet, which contains de-stained collagen fragments and other insoluble materials contained in the test samples (such as bacterial cells or tissues). The amount of dye extracted is quantified by measuring the amount of dye extracted from these fragments, i.e., the absorbance at 600 nm using a spectrophotometer or an ELISA reader.

Apparatus and methods are provided for detecting and monitoring proteolysis of protein matrices such as fibrin clots, extracellular matrix and collagen matrix. The apparatus and methods involve in general the measurement of diffusion-limited electrochemical currents generated by an electroactive species or elactomer in a synthetic protein matrix. The apparatus and methods are applied in various embodiments to detect and monitor the proteolytic activities of a sample, and more specifically fibrinolytic activities and collagenase activities of a sample. The apparatus and methods are utilized generally in the monitoring and diagnostics of cardiovascular, cerebralvascular, and oncology conditions.

The invention provides a collagenase inhibitor, a repair cream containing the collagenase inhibitor and a preparation method of the repair cream. The collagenase inhibitor comprises a ginseng extract and a radix sophorae flavescentis root extract, and based on the total mass of the collagenase inhibitor, the addition amount of the ginseng extract is 51-97%, and the addition amount of the radix sophorae flavescentis root extract is 3-49%. The collagenase inhibitor of the present invention has an excellent inhibiting effect on the activity of collagenase, and has no side effects on the human body.

The invention provides a collagenase inhibitor, a moisturizing crystal dew containing the collagenase inhibitor and a preparation method of the moisturizing crystal dew. The collagenase inhibitor comprises a dendrobium officinale extract and a chamomile extract, and based on the total mass of the collagenase inhibitor, the addition amount of the dendrobium officinale extract is 17-65%, and the addition amount of the chamomile extract is 35-83%. The collagenase inhibitor of the present invention has an excellent inhibitory effect on the activity of collagenase, and has no side effects on the human body.