15 results about "Collagenase activity" patented technology

The invention relates to the technical field of fermentation and purification of clostridium histolyticum collagenase, and discloses a fermentation and purification method of clostridium histolyticum collagenase, which comprises the following steps: step 1, clostridium histolyticum is inoculated into an enrichment medium according to an inoculum size of 2%, the enrichment medium comprises 1g / L of beef extract, 1g / L of peptone, 1g / L of yeast powder, 3g / L of gelatin and 100mL of ultrapure water, and the pH value is 7.4-7.6; step 2, adding 50.5 [mu] L of Fe < 2 + > and 500 [mu] L of Ca < 2 + > into the enrichment culture medium; step 3, performing fermentation culture for 24 hours at 37 DEG C under an anaerobic condition, and performing centrifugation to obtain fermentation supernate. By determining 37 DEG C as the optimal fermentation temperature, 1% nitrogen source concentration and 24-hour fermentation time and innovatively introducing 2.8 mg / 100mL Fe < 2 + > and 10.0 mmol / L Ca < 2 + > as key additives, the clostridium histolyticum collagenase with high expression quantity and high enzyme activity is obtained, the collagenase activity (A-345 <->) is increased from 0.790 U / L to 0.903 U / L, meanwhile, the high protein concentration of 40-45 mg / mL is kept, and the clostridium histolyticum collagenase is suitable for industrial production. Liver and gall tissues of mice can be digested in a short time, and bile duct cells are extracted for organoid culture.

The invention relates to the technical field of biology, in particular to a collagen extraction activity monitoring method based on a refined enzyme extraction technology, which comprises the following steps: preparing collagen labeled by quantum dots and modified by Raman tags into a bimodal probe; a microfluidic monitoring system is constructed based on an integrated reaction chamber, a temperature control module, a pH monitoring module and a signal acquisition module; mixing a sample to be detected with the bimodal probe, adding a structure protective agent, and injecting into a reaction chamber of the microfluidic detection system; synchronously acquiring a fluorescence signal and a Raman signal through the signal acquisition module, and determining to return to the step S2 to recalibrate the signal acquisition module based on a signal-to-noise ratio of the signal; and carrying out fusion analysis on the fluorescence signal and the Raman signal to obtain collagenase activity, triple helix structure integrity and enzymolysis kinetic parameters. The method solves the problem that collagenase activity and collagen triple helix structure integrity data cannot be accurately obtained in a refined enzyme extraction scene.

The present invention relates to a composition comprising plant extracts. The composition according to the present invention has a skin whitening effect by reducing the total amount of melanin and tyrosinase activity in melanocytes of the skin, promotes skin regeneration and increases skin elasticity or reduces skin wrinkles by promoting collagen synthesis and inhibiting collagenase activity in fibroblasts of the skin, has an anti-inflammatory effect or a skin soothing effect by inhibiting NO generation, increases the amount of moisture in the skin and has a moisturizing effect by promoting generation of hyaluronic acid in fibroblasts, and has an antioxidant effect by scavenging free radicals. In addition, since the composition of the present invention exhibits a broad antibacterial effect against various bacteria, the composition may be used as a cosmetic composition, a pharmaceutical composition, a skin external preparation, or a food composition.

The invention discloses a firming and anti-aging composition containing a Campania acuta bark extract and application of the firming and anti-aging composition, and belongs to the technical field of cosmetics. The firming and anti-aging composition containing the Campanumoea maculata bark extract comprises the following components: the Campanumoea maculata bark extract, a Flos Magnoliae Officinalis extract, a Tremella aurantialba extract and a composite polypeptide, and the composite polypeptide is composed of carnosine, acetyl tetrapeptide-5 and tripeptide-10 citrulline in a mass ratio of 1: (0.4-0.6): (0.2-0.6). Through the synergistic effect of the four components, the firming and anti-aging composition has a good effect of inhibiting the activity of elastase and collagenase.

This invention provides an extract of *Elaeocarpus yunnanensis* from Yunnan and its preparation method and application. The preparation method includes the following steps: (1) crushing *Elaeocarpus yunnanensis*, mixing it with an enzyme, and then using a solvent for negative pressure cavitation extraction to obtain a crude extract of *Elaeocarpus yunnanensis*; (2) adsorbing and eluting the crude extract of *Elaeocarpus yunnanensis* obtained in step (1) using a macroporous resin to obtain the *Elaeocarpus yunnanensis* extract. The method provided by this invention can effectively extract active substances from *Elaeocarpus yunnanensis*. The obtained *Elaeocarpus yunnanensis* extract has high geranium content, high total flavonoid content, good antioxidant effect, high hyaluronidase inhibitory activity, good anti-inflammatory effect, and high collagenase activity inhibition rate. The preparation method is economical and environmentally friendly, and the reagents used can be recycled and reused. It can be used as an active substance in health care products and cosmetics.

This invention discloses a method and application for enhancing collagenase activity through amino acid induction, belonging to the field of bio-fermentation. This invention effectively enhances collagenase activity by exogenously adding amino acid inducers during the culture process, thereby significantly increasing the biohydrolysis rate of collagen. This method offers advantages such as high efficiency, environmental friendliness, economy, simple operation, mild culture conditions, and ease of scale-up production.

A composition comprising a polypeptide having collagenase activity, a polypeptide having collagenase activity and method of using said polypeptide for producing GPH-rich collagen tripeptides are provided.

The present invention relates to a fusion protein comprising type I collagen and elastin, and to a use thereof. Specifically, the fusion protein of type I collagen and elastin according to the present invention promotes collagen synthesis and inhibits collagenase activity without showing cytotoxicity to cells, and thus can be usefully used as a functional material for skin improvement.

The application discloses a preparation method of a tea fermentation product with anti-aging efficacy for cosmetics, and obtains a cosmetic yeast strain with cellulase, xylanase and lignin peroxidase production capacity through strain screening. The release of antioxidant and anti-aging substances in tea leaves is improved through fermentation and extraction of tea leaves by the preferred yeast strain, and the anti-aging performance of the fermentation product is further improved through the fermentation of gluconacetobacter kombuchia. Compared with conventional tea extracts, the tea fermentation product has more excellent performance in terms of antioxidant, elastase activity inhibition, collagenase activity inhibition, AGEs yield inhibition, human fibroblast protection and repair, and is suitable for use in skin care products such as essence, emulsion and cream with anti-aging efficacy.

The application discloses a preparation method of lotus stem fermentation product, the lotus stem fermentation product and application thereof, and belongs to the technical field of plant fermentation. The application provides grape Hanseniaspora uvarum which is preserved in the China General Microbiological Culture Collection Center and has a preservation number of CGMCC No. 32471 and a preservation date of November 4, 2024. The suggested classification name is grape Hanseniaspora uvarum. The lotus stem fermentation product is obtained by fermenting lotus stems by using the strain, and has a certain inhibitory effect on collagenase activity.

Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.

The present application relates to the field of biotechnology, and more particularly to a collagen extraction activity monitoring method based on fine enzymatic extraction technology, comprising: preparing a bimodal probe by labeling and modifying collagen with quantum dots and Raman tags; constructing a microfluidic monitoring system based on an integrated reaction chamber, a temperature control module, a pH monitoring module, and a signal acquisition module; mixing the sample to be detected with the bimodal probe, adding a structure protective agent, and then injecting the reaction chamber of the microfluidic detection system; synchronously collecting fluorescence signals and Raman signals through the signal acquisition module, determining whether to recalibrate the signal acquisition module based on the signal-to-noise ratio, and returning to step S2; and performing fusion analysis on the fluorescence signals and Raman signals to obtain collagenase activity, triple helix structure integrity, and enzymatic kinetics parameters. The present application solves the problem of being unable to accurately obtain collagenase activity and collagen triple helix structure integrity data in the fine enzymatic extraction scenario.

The application discloses a firming and anti-aging composition containing a tree bark extract of Eucalyptus globulus and application thereof, and belongs to the technical field of cosmetics. The firming and anti-aging composition containing the tree bark extract of Eucalyptus globulus comprises the following components: a tree bark extract of Eucalyptus globulus, an extract of Jasminum mesnyi, an extract of Tremella fuciformis Berk, and a composite polypeptide, wherein the composite polypeptide is composed of carnosine, acetyl tetrapeptide-5 and tripeptide-10 citrulline with a mass ratio of 1:0.4-0.6:0.2-0.6. The firming and anti-aging composition has a good effect of inhibiting elastase and collagenase activity through the synergistic effect among the four components.

A composition for administration to a subject is disclosed. The composition comprises at least one botanical active component. The botanical active component is present in the composition in an amount effective to inhibit collagenase activity in the skin of the subject. The botanical active component comprises at least one extract selected from the group consisting of: i) an extract of Potentilla glabra leaf; ii) an extract of Bombax malabaricum flower; iii) an extract of Amomum kravanh fruit; iv) an extract of Cornus officinalis fruit; and v) combinations of i) to iv). The composition may be in the form of a topical composition, e.g., for topical application. The composition may also be in the form of an oral composition, e.g., for ingestion. A method of inhibiting collagenase activity in the skin of a subject is also provided. The method comprises administering an effective amount of the composition to the subject.

The invention relates to a nano-drug based on a collagenase infiltration strategy and application of the nano-drug in deep tumor chemotherapy-immune collaborative therapy. Aiming at the problems of insufficient permeation, low accumulation rate and large toxic and side effects of traditional chemotherapeutic drugs caused by a shielding effect of a tumor tissue microenvironment on the nano-drugs, bovine serum albumin is used as a carrier, collagenase and low-dose docetaxel are co-entrapped through a self-assembly method, and a nano-particle drug (COL / DTX-coated BSA NPs) is constructed. The nano-drug has the characteristics of uniform spherical morphology, high encapsulation efficiency and drug loading capacity, good collagenase activity maintenance, excellent biocompatibility and the like, and collagenase-mediated tumor penetration enhancement, a low-dose chemotherapy-immune synergistic mechanism and safety guarantee can be realized. Tumor targeted enrichment and deep penetration are realized in a breast cancer model, chemotherapy tumor inhibition effect and anti-tumor immunity are synergistically enhanced, system toxicity is remarkably reduced, and a new strategy is provided for high-efficiency and low-toxicity treatment of solid tumors.