Extracellular polysaccharide for discoloring water-soluble dye wastewater and application thereof as coagulant aid

A technology of bacterial exopolysaccharide and coagulant aid, applied in bacteria, microorganism-based methods, textile industry wastewater treatment, etc., can solve the problems of high cost, poor biodegradability, and low wastewater efficiency, and achieve convenient storage and sugar production. The effect of high volume and increased floc size

Inactive Publication Date: 2010-12-22
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem to be solved by the present invention is to overcome the disadvantages of low efficiency of using inorganic coagulants alone to treat water-soluble dye wastewater and

Method used

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  • Extracellular polysaccharide for discoloring water-soluble dye wastewater and application thereof as coagulant aid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Cultivation of Pseudoalteromonas.sp.SM20310 strain

[0028] Strain: Pseudoalteromonas.sp.SM20310, deposit number: CCTCC NO: M 209258, depository unit: China Center for Type Culture Collection, deposit date: November 9, 2009.

[0029] The Pseudoalteromonas.sp.SM20310 strain was inoculated into the 2216E liquid culture base at 15°C, and cultured in a shaker at 150rpm in the dark for 72h to ferment and produce sugar. The strain inoculation amount is 5wt%.

[0030] 2216E liquid medium formula: 1g of yeast extract, 5g of peptone, 30g of glucose, 1L of seawater for 2 months, adjust the pH to 7.5 with NaOH.

[0031] or:

[0032] The Pseudoalteromonas.sp.SM20310 strain was inoculated into the fermentation culture based on 15°C, and cultured in a shaker at 150rpm in the dark for 72h to ferment to produce sugar. . The inoculum amount of the strain is 6wt%.

[0033] Fermentation medium formula: corn flour 20g, bran 10g, soybean meal 20g, Na 2 HPO 4 1g, KH 2 PO ...

Embodiment 2

[0034] Example 2: Preparation of Pseudoalteromonas.sp.SM20310 exopolysaccharide

[0035] The fermented liquid obtained in Example 1 was collected and centrifuged at 11000 rpm at 15° C. for 15 min to obtain a supernatant. Add 1:1-2:1 (v / v) absolute ethanol to the supernatant, centrifuge after the extracellular polymer is precipitated, dissolve in water, then concentrate, add 20%-25% (v%) of Chloroform and 4%-5% (v%) butanol were stirred for 20 minutes, centrifuged at 15° C. and 10,000 rpm for 10 minutes to remove protein, and the supernatant was collected. After concentration, put it in a 14000U dialysis bag for dialysis and desalination, add 1:1~2:1 (v / v) absolute ethanol to the supernatant again, separate out the polysaccharide and then centrifuge and dry or lyophilize at normal temperature to obtain The bacterial exopolysaccharide referred to in the present invention is stored at 4°C. Before use, make it into a 0.5-3g / L solution and store it at 4°C for future use.

Embodiment 3

[0036] Embodiment 3: the application of bacterial exopolysaccharide as coagulation aid

[0037] The comparison experiment of the decolorization rate of reactive brilliant red before and after adding bacterial exopolysaccharide coagulant aid at different pH is as follows:

[0038] Take the reactive brilliant red X-3B dye solution with a concentration of 100mg / L and place it in a shaker at 25°C, and use 1mol / L NaOH or HCl to adjust the initial pH of the solution to 8, 9, 9.5, 10, 10.3, 10.6, respectively. Add ferric chloride containing 55mg / L Fe(III), shake rapidly at 200rpm for 2min, then shake slowly at 60rpm for 17min, let stand for 30min, take the supernatant to measure its absorbance and calculate the decolorization rate of ferric chloride. Add the bacterial exopolysaccharide coagulant of embodiment 2 after the foregoing operation is shaken at a slow speed for 17 minutes, so that the concentration of the bacterial exopolysaccharide in the mixed solution reaches 150 mg / L, co...

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Abstract

The invention relates to an extracellular polysaccharide coagulant aid for discoloring water-soluble dye wastewater, successfully solving the problems of low efficiency of processing the water-soluble dye wastewater by independently using inorganic coagulant aids as well as high cost and poor biodegradation of artificially synthesized high-polymer coagulant aid by compounding with various inorganic coagulant aids. The preparation method of the extracellular polysaccharide as the coagulant aid comprises the following steps of: firstly inoculating the strains of Pseudoalteromonas.sp.SM20310 of Antarctic sea ice bacteria in a 2216E fluid medium or a fermentation medium, fermenting for 72 h at 15 DEG C under the condition of 150-rpm light avoidance, and collecting a fermentation fluid; extracting an extracellular polymer of the fermentation fluid by utilizing an alcohol precipitation method, and then removing proteins by using an Sevag method to obtain a crude product of the extracellular polysaccharide; and drying and storing the dried crude product, compounding the crude product to a solution with a certain concentration before being used, and then adding by adopting a wet method. When being used for processing the water-soluble dye wastewater, the extracellular polysaccharide as the coagulant aid is added after the inorganic coagulant aids are added, thereby higher floc settling speed and wastewater discoloring efficiency can be obtained.

Description

technical field [0001] The invention relates to a bacterial exopolysaccharide coagulant that can effectively improve the decolorization effect of water-soluble dye wastewater, and belongs to the technical field of dye wastewater decolorization. Background technique [0002] Water-soluble dye wastewater has the characteristics of large water volume, high chroma, and complex composition. After being discharged into the water body, it will not only bring discomfort to the human senses, but also reduce the light transmittance of the water body and affect the normal growth of aquatic organisms, and even cause harm to the human body. produce triple effect. In addition to dyes, the wastewater also contains a large amount of non-biodegradable organic additives and heavy metal ions such as copper, chromium, and zinc, so it needs to be properly treated before it can be discharged. [0003] The treatment of dye wastewater mainly includes coagulation method, adsorption method, oxidatio...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C02F1/56C12R1/01C02F103/30
Inventor 周维芝张玉忠陈波申博玲刘升波孟凡平俞勇陈秀兰周百成
Owner SHANDONG UNIV
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