New Poria cocos strain and liquid fermentation method thereof

A technology of liquid fermentation and poria cocos, applied in the field of liquid fermentation of medicinal fungi, to achieve the effect of increasing the content of exopolysaccharide, increasing the level, good production performance and stability

Inactive Publication Date: 2011-09-28
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there is no literature report on the method of adding traditional Chinese medicine to the culture medium to improve the fermentation level of Poria cocos

Method used

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  • New Poria cocos strain and liquid fermentation method thereof
  • New Poria cocos strain and liquid fermentation method thereof
  • New Poria cocos strain and liquid fermentation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The breeding of embodiment 1 excellent bacterial strain of Poria cocos

[0030] 1. Medium

[0031] Complete medium (TYEG medium): tryptone 10g, yeast extract 5g, K 2 HPO 4 3g, glucose 1g, agar 15g, distilled water 1L, pH 7.0. 1.05kg / cm 2 , sterilized at 121.3°C for 20 minutes.

[0032] Liquid fermented seed medium: glucose 20g, yeast extract 4g, peptone 5g, K 2 HPO 4 1g, MgSO 4 .7H 2 O 0.5g, initial pH 5.5, distilled water 1L. 1.05kg / cm 2 , sterilized at 121.3°C for 20 minutes.

[0033] Liquid fermentation medium: glucose 20g, yeast extract 3.5g, peptone 4.5g, K 2 HPO 41g, MgSO 4 .7H 2 O 0.5g, initial pH 5.5, distilled water 1L. 1.05kg / cm 2 , sterilized at 121.3°C for 20 minutes.

[0034] 2. Mutation breeding

[0035] 1) UV mutagenesis

[0036] a, Preparation of spore suspension Take the typical strain of Poria cocos with the preservation number P0, place it in sterile physiological saline, prepare a spore suspension, pour it into a small triangular f...

Embodiment 2

[0054] The influence of embodiment 2 Chinese medicines on the liquid fermentation of Poria cocos shake bottle

[0055] 1. Medium

[0056] Seed medium: glucose 20g, yeast extract 4g, peptone 5g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.5g, distilled water 1L, initial pH 5.5.

[0057] Fermentation base medium: glucose 20g, yeast extract 3.5g, peptone 4.5g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.5g, distilled water 1L, initial pH 5.5.

[0058] 2. Fermentation method

[0059] 1), the preparation of Poria cocos shake flask liquid fermentation strain

[0060] Put 50mL seed culture medium into a 250mL Erlenmeyer flask, inoculate the Poria cocos strain with the preservation number CCTCC NO: M 2010361 obtained in Example 1 at a ratio of 0.5%, shake the flask at 25°C for 2 days and use it as a liquid fermentation strain for later use.

[0061] 2), inoculation culture

[0062] In the shake flask liquid fermentation basal medium, add coix seed and other 9 traditional Chinese medicine solid po...

Embodiment 3

[0073] The influence of embodiment 3 different Chinese medicine dosages on Poria cocos shake flask liquid fermentation

[0074] 1. Medium

[0075] Seed medium: glucose 20g, yeast extract 4g, peptone 5g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.5g, distilled water 1L, initial pH 5.5.

[0076] Fermentation base medium: glucose 20g, yeast extract 3.5g, peptone 4.5g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.5g, distilled water 1L, initial pH 5.5.

[0077] 2. Fermentation method

[0078] 1), preparation of Poria cocos shake flask liquid fermentation strain

[0079] Put 50mL seed culture medium into a 250mL Erlenmeyer flask, inoculate the Poria cocos strain with the preservation number CCTCC NO: M 2010361 obtained in Example 1 at a ratio of 0.5%, shake the flask at 25°C for 2 days, and use it as a liquid fermentation strain for later use.

[0080] 2), inoculation culture

[0081] Coix seed, licorice, light bamboo leaf, mulberry leaf, Ganoderma lucidum and Lycium barbarum solid powder (the ...

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Abstract

The invention provides a new Poria cocos strain and a liquid fermentation method thereof and relates to the field of Poria cocos liquid fermentation. The invention provides the Poria cocos strain conserved in the China center for type culture collection, wherein the CCTCC NO is M 2010361. The invention also provides a Poria cocos liquid fermentation method and the method comprises the following steps: a) preparing a fermentation seed: inoculating the Poria cocos strain of which CCTCC NO is M 2010361 in seed culture medium to obtain the Poria cocos seed; and b) fermenting: inoculating the seed obtained in the step a) in Poria cocos liquid fermentation culture medium to ferment and obtain fermentation liquor, namely the Poria cocos fermentation product. By adopting the fermentation method, the mycelium yield or extracellular polysaccharide yield of Poria cocos liquid fermentation can be increased, the demand of the market on Poria cocos can be met and a foundation is laid for the developments of the health food and medicine which are related to Poria cocos.

Description

technical field [0001] The invention relates to the field of liquid fermentation of medicinal fungi, in particular to the liquid fermentation of poria cocos. Background technique [0002] Poria cocos is a perennial saprophytic fungus, which belongs to the Phylum Fungi, Class Basidiomycetes, Subclass Nonseptabasidiomycetes, Polyporaceae, Polyporaceae, and the genus Polyporia. Poria cocos was first recorded in "Shen Nong's Materia Medica" and was listed as top grade. There are records of the application of Poria cocos as early as the late Spring and Autumn Period in my country, and the history of artificial cultivation of Poria cocos has been more than 1,500 years. Traditional Chinese medicine believes that Poria cocos "diures water but does not hurt the body, nourishes but does not help evil", and can promote water and dampness, invigorate the spleen and calm the nerves; modern medical research shows that the pharmacological effects of Poria cocos are mainly manifested in di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/04C12R1/645
Inventor 李羿钟世红游元元李晨
Owner CHENGDU MEDICAL COLLEGE
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