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Application of lachnum extracellular polysaccharide phosphorylated derivative and application thereof in preparation of antitumor drugs

An extracellular polysaccharide, Trichococcus granulosa technology, applied in the directions of antitumor drugs, drug combinations, pharmaceutical formulations, etc., to achieve the effects of no toxic side effects, significant antitumor function, and a simple and economical preparation method

Inactive Publication Date: 2013-09-25
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no public literature mentioning the phosphorylated derivatives of Trichomonas exopolysaccharide and its application as an anti-tumor drug.

Method used

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  • Application of lachnum extracellular polysaccharide phosphorylated derivative and application thereof in preparation of antitumor drugs

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Preparation of exopolysaccharide from Trichodia granulosa:

[0016] The culture preservation number is CCTCC No: M2011196 Trichomonas inoculated in 5L fermenter, medium formula is: glucose 40g / L, yeast extract 12g / L, glycine 6.2mg / L, MgSO4 7H2O 1g / L, KH2PO4 1g / L, the volume ratio of the loading factor is 0.7, the inoculum size is 15% (volume ratio), the culture temperature is 35°C, the ventilation rate is 1.0L / min, the stirring speed is 180r / min, and the fermentation time is 10 days; After suction filtering and concentrating the fermentation broth, add 3 times its volume of ethanol with a mass fraction of 95% and alcohol precipitation at 4°C for 24 hours, centrifuge the ethanol solution at 5000r / min for 10min, remove the supernatant, and precipitate with absolute ethanol, Wash with acetone for 2 to 3 times, then dissolve with distilled water, deproteinize by Sevag method and decolorize with 30% H2O2 (50°C) by volume percent concentration, dialyze with distilled water...

Embodiment 2

[0052] Firstly, prepare and obtain the exopolysaccharide of Trichodia granulosa according to the following conditions and methods: inoculate Trichodia granulosa exopolysaccharide with strain preservation number CCTCC No: M2011196 in a 4L fermenter, and the medium formula is: glucose 44g / L, yeast extract 12g / L, glycine 6.5mg / L, MgSO4 7H2O 1.2g / L, KH2PO4 1.2g / L, the volume ratio of the loading coefficient is 0.6, the inoculum size is 14% (volume ratio), the culture temperature is 30°C, and the ventilation rate is 1.1L / min, the stirring speed is 175r / min, and the fermentation time is 8 days; after the fermentation broth is suction-filtered and concentrated, 2 times its volume of ethanol with a mass fraction of 95% is added to alcohol precipitation at 4°C for 30h, and the alcohol is separated The solution was centrifuged at 5000r / min for 8min, the supernatant was removed, the precipitate was washed 3 times with absolute ethanol and acetone, and then dissolved in distilled water, d...

Embodiment 3

[0057] Example 3: Processing of Phosphorylated Derivatives of Exopolysaccharides from Trichomonas granulosa

[0058] In the present invention, the exopolysaccharide phosphorylated derivatives produced by Trichodia granulosa with the strain preservation number of CCTCC No: M2011196 are used as the preparation of anti-tumor drugs, which can be formulated and processed into the following dosage forms for use:

[0059] 1. Tablet processing

[0060] Mix 300-600g of phosphorylated exopolysaccharide derivatives of Trichodia granulosa with 200-250g of pharmaceutical excipient diluent and 30-60g of glidant, and then mix them with 250-600mL of ethanol to obtain a soft material. Wet granules are obtained by sieving, granulated after drying, and finally 3-10 g of lubricant is added, mixed evenly, and compressed into tablets to make 1000 tablets. The pharmaceutical excipient diluent is dextrin or dry starch; the glidant is talcum powder or micropowder silica gel or microcrystalline cellul...

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Abstract

The invention discloses an application of lachnum extracellular polysaccharide phosphorylated derivative and an application thereof in preparation of antitumor drugs. The lachnum extracellular polysaccharide phosphorylated derivative is obtained by performing phosphorylated decoration on extracellular polysaccharide generated by lachnum with strain preservation number of CCTCC No: M2011196. As shown by an experiment, the extracellular polysaccharide phosphorylated derivative generated by lachnum with strain preservation number of CCTCC No: M2011196 not only has excellent antitumor function, but also has no toxicity or side effect, and the preparation method is simple and economic, so that the lachnum extracellular polysaccharide phosphorylated derivative can be used for preparing tablet or capsule antitumor drugs.

Description

technical field [0001] The invention relates to a phosphorylated derivative of the extracellular polysaccharide of Trichomona granulosa and its application in the preparation of antitumor drugs. Background technique [0002] Malignant tumor is one of the common clinical diseases, and it is also one of the main causes of human death. At present, the treatment of malignant tumors mainly adopts methods such as radiotherapy and chemotherapy. The commonly used chemotherapeutic drugs are mostly chemically synthesized substances rather than natural products. Therefore, while killing tumor cells, they also have a certain degree of killing effect on normal cells; and simple Chemotherapy can cause loss of appetite and weight loss in patients, leading to weakening of the body, and causing a certain degree of immunosuppression, with relatively large toxic and side effects. Therefore, excavating and developing natural, non-toxic anti-tumor drugs is of great significance for maintaining ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 叶明袁如月杜湛湛
Owner HEFEI UNIV OF TECH
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