Preparation method for intensive pulse light treated cumaldehyde/tetra-lysine antibacterial agent

A technology of pulsed strong light and tetralysine, which is applied in the directions of botanical equipment and methods, biocides, disinfectants, etc., can solve the problems of sterilization and fresh-keeping biofilms being difficult to remove, and achieves a simple and economical preparation method, good effect and large scale. The effect of development prospects

Pending Publication Date: 2020-05-08
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of sterilization and preservation of food and the problem that biofilm on medical

Method used

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  • Preparation method for intensive pulse light treated cumaldehyde/tetra-lysine antibacterial agent
  • Preparation method for intensive pulse light treated cumaldehyde/tetra-lysine antibacterial agent
  • Preparation method for intensive pulse light treated cumaldehyde/tetra-lysine antibacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1 Antibacterial effect of cumaldehyde / tetralysine antibacterial agent treated with pulsed strong light on E.coli and E. coli biofilm

[0019] 1 Experimental bacteria

[0020] Foodborne pathogens: Escherichia coli

[0021] 2 Experimental equipment

[0022] Vertical pressure steam sterilizer HVE50 Hirayama Co., Ltd., Japan

[0023] Biochemical incubator LRH Shanghai Yiheng Scientific Instrument Co., Ltd.

[0024] 3 experimental methods

[0025] 1) In vitro antibacterial effect of cumaldehyde / tetralysine antibacterial agent treated with pulsed light on Escherichia coli

[0026] ① E. coli was inoculated into nutrient broth liquid medium and cultured at 37°C with shaking (150 rpm) for 24 hours to obtain E. coli in logarithmic growth phase.

[0027] ②The Escherichia coli in the logarithmic growth phase was serially diluted and added to 5mL sterile phosphate buffer to make the concentration of Escherichia coli 10 4 -10 5 CFU / mL, and then with 5mL 0.6wt% tetralysine antibacterial age...

Example Embodiment

[0036] Example 2 Antibacterial effect of tetralysine on Staphylococcus aureus (S.aureus) and Staphylococcus aureus biofilm

[0037] 1 Experimental bacteria

[0038] Staphylococcus aureus (S.aureus)

[0039] 2 Experimental methods

[0040] 1) The antibacterial effect of cumaldehyde / tetralysine antibacterial agent treated with pulsed light on S.aureus in vitro

[0041] ① S. aureus was inoculated into nutrient broth liquid medium and cultured at 37°C with shaking (150 rpm) for 48 hours to obtain logarithmic growth phase of Staphylococcus aureus (S. aureus).

[0042] ②Add S.aureus in the logarithmic growth phase to sterile phosphate buffer, and dilute it to 10 4 -10 5 CFU / mL, and then add the same amount of cuminal / tetralysine antibacterial agent, tetralysine and cuminal treated with pulsed strong light.

[0043] ③Cultivate the bacterial suspension with the antibacterial agent at 37°C for 24 hours, and then use the plate counting method to determine the number of remaining bacteria. The S. a...

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Abstract

The invention belongs to the field of food antibacterial additives, in particular to a preparation method for an intensive pulse light treated cumaldehyde/tetra-lysine antibacterial agent. According to the antibacterial agent prepared by the preparation method, firstly, tetra-lysine is mixed with cumaldehyde, ethanol and sterile distilled water according to a certain proportion; magnetic stirringfor dissolution is performed to obtain a compound antibacterial agent solution; and then, the compound antibacterial agent solution is treated with intensive pulse light to obtain the intensive pulselight treated cumaldehyde/tetra-lysine antibacterial agent. The preparation method is simple, and the prepared antibacterial agent is safe and non-toxic, has the biological film inhibiting feature andrelatively high antibacterial activity, can effectively inhibit growth of all kinds of escherichia coli and biological films thereof and growth of staphylococcus aureus and biological films thereof,and has very good market application value in the field of food safety.

Description

technical field [0001] The invention belongs to the field of food antibacterial additives, and in particular relates to a preparation method of a cuminaldehyde / tetralysine antibacterial agent treated with pulsed strong light. Background technique [0002] With the improvement of living standards and the advancement of science and technology, people pay more and more attention to food hygiene and safety, which promotes the development of antibacterial agents. Safe and non-toxic, antibacterial agents with high antibacterial efficiency are favored by people. [0003] Peptide is one of the important material bases of life, and its function involves every link in the life process. Tetrapeptide is a protein fragment or substance composed of amide bonds (ie -CO-NH-) formed by dehydration condensation between amino acid molecules. Tetralysine is a tetrapeptide composed of four lysine residues, belonging to short cationic tetrapeptides. Because it is composed of amino acids, it is...

Claims

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Application Information

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IPC IPC(8): A23L3/3499A23L3/3526A01N35/04A01N37/46A01P1/00
CPCA01N35/04A01N37/46A23L3/3499A23L3/3526A23V2002/00A23V2200/10A23V2250/30A23V2250/55
Inventor 林琳廖雪崔海英周昌倩
Owner JIANGSU UNIV
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