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Method for producing extracellular mucopoly-saccharide using rhamnose lactobacillus and product

A technology of Lactobacillus rhamnosus and mucopolysaccharides, applied in the field of functional food and pharmaceutical products

Inactive Publication Date: 2006-06-07
天津市工业微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no academic and industrial reports on the exopolysaccharide of Lactobacillus rhamnosus in my country

Method used

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  • Method for producing extracellular mucopoly-saccharide using rhamnose lactobacillus and product

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Experimental program
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specific Embodiment approach

[0017] The specific embodiment: the specific implementation method steps of the present invention are as follows:

[0018] (1) Technological process: See attached drawing 1.

[0019] (2) Operation points:

[0020] ① Strains: Lactobacillus rhamnosus (L.R-008) is cultured on a slant, in a triangular flask, and expanded in a seed tank, and then connected to a fermenter for fermentation.

[0021] ② Fermentation raw materials: whey powder, glucose, diamine hydrogen citrate, MgSO 4 ·7H 2 O, MnSO 4 ·H 2 O. Tween-80.

[0022] ③ Ingredients: Fermentation raw materials are put into the fermentation tank according to a certain ratio of ingredients. The specific proportions are: whey powder 5% to 10%, glucose 1% to 5%, diamine hydrogen citrate 0.1% to 1%, MgSO 4 ·7H 2 O0.02%~0.1%, MnSO 4 ·H 2 O0.01%~0.1%, Tween-80 1ml / L~2ml / L, the balance is water;

[0023] ④ Fermentation: use glucose and lactose as carbon sources, maintain pH 5.5-6.0, and ferment at 32°C to 37°C for 24 hours t...

Embodiment 1

[0037] Key points of operation: See Figure 1 for the process flow

[0038] ① Expanded culture of strains: Lactobacillus rhamnosus L.R-008 was cultured on a slant, cultured in a triangular flask, continued to expand the culture in the seed tank, and then connected to a fermenter for fermentation.

[0039] ② Fermentation raw materials: whey powder, glucose, diamine hydrogen citrate, MgSO 4 ·7H 2 O, MnSO 4 ·H 2 O. Tween-80.

[0040] ③ Ingredients: Fermentation raw materials are put into the fermentation tank according to a certain ratio of ingredients. The specific ratio is: whey powder 5%, glucose 1.5%, diamine hydrogen citrate 0.2%, MgSO 4 ·7H 2O0.04%, MnSO 4 ·H 2 O0.04%, Tween-80 1ml / L, the balance is water;

[0041] ④Fermentation: After the above-mentioned raw materials are prepared, the inoculation amount is 3% (v / v), and the pH is maintained at 5.5-6.0, and fermented at 34°C to 37°C for 50 hours. The fermenter was stirred intermittently at 100r / min without supple...

Embodiment 2

[0050] Key points of operation: See Figure 1 for the process flow

[0051] ① Expanded culture of strains: Lactobacillus rhamnosus L.R-008 was cultured on a slant, cultured in a triangular flask, continued to expand the culture in the seed tank, and then connected to a fermenter for fermentation.

[0052] ② Fermentation raw materials: whey powder, glucose, diamine hydrogen citrate, MgSO 4 ·7H 2 O, MnSO 4 ·H 2 O. Tween-80.

[0053] ③ Ingredients: Fermentation raw materials are put into the fermentation tank according to a certain ratio of ingredients. The specific ratio is: whey powder 8%, glucose 0.5%, diamine hydrogen citrate 0.5%, MgSO 4 ·7H 2 O0.06%, MnSO 4 ·H 2 O0.02%, Tween-80 1.5ml / L, the balance is water;

[0054] ④Fermentation: After the above-mentioned raw materials are prepared, the inoculation amount is 3% (v / v), and the pH is maintained at 5.5-6.0, and the fermentation is carried out at 32°C to 35°C for 68 hours. The fermenter was stirred intermittently a...

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Abstract

The present invention relates to process of producing extracellular mucopolysaccharide with rhamnose lactobacillus as initial strain and glucose and lactose as carbon source and including the steps of compounding material, expanding culturing and fermentation, separation, washing, dewatering and drying. The extracellular mucopolysaccharide consists of pyruvic acid in 1 weight portion, D-glucose in 2 weight portions, D-galactose in 1 weight portion and L-rhamnose in 4 weight portions, and is colorless and smell-less solid of molecular weight 30-60 MDa. Compared with other kinds of lactobacillus extracellular polysaccharide, rhamnose lactobacillus extracellular mucopolysaccharide has higher fermentation yield, the biological effects of resisting tumor, raising immunity, resisting blood coagulation, resisting virus, etc. and wide application in food, medicine, papermaking, preservation and other fields.

Description

technical field [0001] The present invention uses a lactic acid bacteria microorganism as the starting bacterium, which can produce and secrete extracellular viscous polysaccharide (Lactobacillus rhamnosus EPS) under fermentation conditions. The present invention also relates to the application of the viscous polysaccharide in food, beverage and diet Human functional food and pharmaceutical products. Background technique [0002] Polysaccharides are polymers of aldose and / or ketose sugars that exist in nature linked together by glycosidic bonds. Polysaccharides are essential components of all living organisms, and they are closely related to various physiological functions that sustain life. Polysaccharides have appeared as an important type of biologically active natural products, and various polysaccharides have been found to have anti-tumor, immune-enhancing, anti-coagulant, hypoglycemic and anti-viral activities. [0003] Most of the extraction and separati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12N1/20C12P1/00C12P19/04
Inventor 张刚奚震叶樱孙凤雄
Owner 天津市工业微生物研究所有限公司
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