Paenibacillus polymyxa extracellular polysaccharide and application thereof

A technology of spore-like polymyxa and exopolysaccharide, which is applied in the biological field and can solve problems such as applications that have not been reported yet

Inactive Publication Date: 2010-10-27
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although there are many studies on the application of Paenibacillus polymyxa exopolysaccharide, there are no reports on its application as a suspension aid and protection of live bacteria

Method used

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  • Paenibacillus polymyxa extracellular polysaccharide and application thereof
  • Paenibacillus polymyxa extracellular polysaccharide and application thereof
  • Paenibacillus polymyxa extracellular polysaccharide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1 Paenibacillus polymyxa high viscosity culture technique

[0017] In this example, the high-viscosity culture process of Paenibacillus polymyxa was realized by optimizing the medium components and changing the culture conditions.

[0018] method:

[0019] (1) Optimization of culture medium

[0020] In this example, suitable carbon sources are selected from starch, dextrin, glycerin, glucose, sucrose, rice flour, molasses, sorghum, and the like. Screen the appropriate nitrogen source from silkworm chrysalis powder, soybean powder, peanut cake powder, protein powder, meat extract, rice sugar, wheat bran, yeast powder, corn steep liquor, ammonium salt, etc. Select suitable inorganic salts from sodium chloride, phosphate, ammonium sulfate, manganese sulfate, magnesium sulfate, calcium carbonate and other metal salts.

[0021] The viscosity of the fermentation broth and the content of exopolysaccharide are used as screening criteria.

[0022] The screening st...

Embodiment 2

[0044] Example 2 Separation and Purification of Paenibacillus polymyxa exopolysaccharide

[0045] In this example, the high-viscosity fermentation broth of Paenibacillus polymyxa was subjected to alcohol precipitation, centrifugation, decolorization, protein removal, column chromatography, and finally freeze-drying to obtain relatively pure polysaccharides.

[0046]Paenibacillus polymyxa was cultured according to the cultivation process described in Example 1 to obtain a high-viscosity Paenibacillus polymyxa fermentation broth. Then the above-mentioned fermentation broth is gradually subjected to alcohol precipitation (fermentation broth: ethanol = 1: 1 to 5 ratio (volume ratio) mixed, fully stirred and then left to stand for 12 to 36 hours), centrifuged (centrifuged at 5000r / min or more to take the precipitate) or Filtration (reduced pressure filtration), decolorization (activated carbon adsorption decolorization or / and dialysis (can pass through a dialysis bag with a molecul...

Embodiment 3

[0048] Example 3 Application Research of Paenibacillus polymyxa exopolysaccharide

[0049] (1) Suspending effect

[0050] Using the Paenibacillus polymyxa exopolysaccharide obtained in Example 2, according to the determination method of the suspension rate, the suspension-assisting effect of the exopolysaccharide was investigated, and the specific data are shown in Table 5, Table 6 and Table 7.

[0051] Table 5 takes kaolin as the suspension aiding effect of test object determination

[0052]

[0053] Table 6 takes calcium carbonate as the suspension-assisting effect measured by the test object

[0054]

[0055] Table 7 takes diatomaceous earth as the suspension-assisting effect of test object determination

[0056]

[0057] From the above data in Tables 5, 6, and 7, it can be seen that the addition of Paenibacillus polymyxa exopolysaccharide can obviously promote the suspension rate of kaolin, calcium carbonate, and diatomite, that is, the Paenibacillus polymyxa ex...

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Abstract

The invention belongs to the field of biotechnology, in particular to Paenibacillus polymyxa extracellular polysaccharides and application thereof. The extracellular polysaccharide has strong auxiliary suspension effect on diatomite, kaoline and calcium carbonate and protective action on live bacteria. The Paenibacillus polymyxa extracellular polysaccharide has wide application prospect in the aspects of processing and use of pesticides, medicaments, food, petroleum extraction, water treatment, microorganism live bacteria preparations.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a Paenibacillus polymyxa exopolysaccharide and its application. The exopolysaccharide not only has a strong suspending effect on diatomite, kaolin, and calcium carbonate, but also has a Has a protective effect. The Paenibacillus polymyxa exopolysaccharide has broad application prospects in the fields of pesticides, medicine, food, oil exploitation, water treatment, and the processing and use of live microbial preparations. Background technique [0002] Paenibacillus polymyxa is widely used in fields such as agriculture, industry and water treatment. In recent years, there have been many researches on the application of Bacillus polymyxa exopolysaccharide at home and abroad, mainly focusing on flocculation, adsorption and leaching of metal ions, and mineral processing. For example, Yang Zhaohui from Hunan University and Gong Xiaoyan from the Eco-Environmental Research Cen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 李元广沈国敏魏鸿刚张道敬罗远婵王伟良
Owner EAST CHINA UNIV OF SCI & TECH
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