Strain for preventing and curing muskmelon bacteroidal spot disease and application thereof in field disease prevention

A bacterial and spot disease technology, applied in the application, bacteria, fungicides and other directions, can solve the problems of destroying the ecological environment, increasing the burden on farmers, endangering human health, etc., to achieve no pollution to the environment, no harm to human health, and safe and reliable prevention and control. Effect

Inactive Publication Date: 2008-07-09
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, in the traditional agricultural pest control, many people have formed a habit of chemical pesticides to control crop pests and diseases. Although it has achieved a certain control effect, its negative impact is becoming more and more serious
As the damage of diseases and insect pests of major crops increases year by year, a large amount of pesticides sprayed every year not only increases the burden on farmers, increases production but does not increase income, but also seriously damages the ecological environment on which human beings depend for survival, and also causes pesticide residues in food, endangering human health
So far, there have been many relevant reports on the research on the biological control of plant fungal diseases, but there are few researches on the biological control of bacterial diseases, and there are very few domestic and foreign research reports on the biological control of bacterial spot of melon. Therefore, The biological control method with functional microorganisms as the core has broad prospects and has become a research hotspot at home and abroad

Method used

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  • Strain for preventing and curing muskmelon bacteroidal spot disease and application thereof in field disease prevention
  • Strain for preventing and curing muskmelon bacteroidal spot disease and application thereof in field disease prevention
  • Strain for preventing and curing muskmelon bacteroidal spot disease and application thereof in field disease prevention

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the primary screening method of antagonistic bacteria

[0027] Draw 1mL of pathogenic bacteria diluted fermented liquid (hemocytometer and dilute to 1×10 7 1 / mL), 1 mL of different gradient soil dilutions were mixed in a sterilized empty test tube, and then 0.1 mL of the mixed solution was drawn and spread on a plate, and cultured upside down (28 °C, 48 h) to observe whether there was a zone of inhibition , record the size of the production circle, and pick a single colony in the production circle and use the plate streaking method to purify it, cultivate it at 28°C, and when a single colony grows on the medium, it is a pure strain, and inoculate the obtained single colony on the improved On the slant medium, cultured at 28°C for 48h, then stored in a refrigerator at 4°C for future use, see Figure 1.

[0028] A total of 195 strains producing inhibition zones were screened from 86 different soil samples by the test tube mixed bacteria plate coating method....

Embodiment 2

[0033] Embodiment 2: the double screening method of antagonistic bacteria

[0034] Select the strains with a bacteriostatic distance of more than 10 mm for re-screening by tube-and-disk method. Pathogenic bacteria suspension (1 × 10 7 each / mL) pipette 0.1mL and spread it on the plate, and use a sterilized hole puncher to punch holes on the plate, then add the antagonistic bacteria fermentation broth (fermentation culture at 28°C for 48h) into the punched holes, and then place in the incubator Medium culture (28°C, 48h) to observe the size of the inhibition zone, repeated three times.

[0035] The highly efficient and stable G-14 was screened from 63 isolates of antagonistic bacteria by the tube-and-disc method, and its antagonistic effect is shown in Figure 2.

Embodiment 3

[0036] Embodiment 3: the influence of temperature on the growth of Paenibacillus polymyxa (Paenibacillus polymyxa)

[0037] Inoculate G-14 into the liquid basal medium, the inoculum amount is the same as that of the growth curve test, culture in a shaker at 20°C, 25°C, 28°C, 30°C, and 37°C, with a rotation speed of 180r / min, until the stable stage, and use UV The -2550 spectrophotometer measures the OD value at a wavelength of 600nm, sets the non-inoculated culture solution as the control, and repeats it several times.

[0038] It is known from the above examples that the optimum growth and culture temperature of the bacterial strain of the present invention has antibacterial activity in the range of 20-37°C, and its optimum action temperature is 25-30°C.

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Abstract

The invention discloses a biological prevention strain of Paenibacillus polymyxa CGMCC.No.2250 and an application in the muskmelon bacterial spot disease treatment. The provided strain of the invention and the strain and variation strain that equals the strain in the invention on the mycology can be effectively applied in the prevention of muskmelon bacterial spot disease and the prevention disposal is prior to the treatment disposal. By using the strain, the prevention is safe and reliable without harming human health and polluting environment and is good in effect, low in cost, safe in use without polluting environment and the like compared with traditional chemical prevention, which can be widely applied in the industrial or agricultural biological control field.

Description

field of invention [0001] The invention relates to the field of biological control, in particular, the invention relates to a strain for preventing and treating bacterial spot of melon and its application in field disease prevention. Background technique [0002] Hami melon (commonly known as muskmelon) is one of the well-known Xinjiang specialties. It occupies an important position in the production of muskmelon in my country and enjoys a high reputation both at home and abroad. However, in the past ten years, bacterial diseases of melons have been the main obstacle restricting the production and development of melons in Xinjiang. Pseudomonassyringae pv lachrymans and Acidovorax avenae subsp. Serious disease in Hami melon production. The general incidence rate of this disease is 30-40%, and the severe cases reach 80%, and even cause the whole field to fail, especially in rainy years in June and July, it can often cause devastating losses. [0003] In 1965, Webb et al. fir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/21A01N63/02A01P3/00C12R1/12
Inventor 王杏琴史应武刘文玉娄恺
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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