Method for preparing functional extracellular polysaccharide of lactic acid bacteria

A technology of exopolysaccharides and lactic acid bacteria, which is applied in the field of preparation of functional lactic acid bacteria exopolysaccharides, can solve the problems that have not been reported, and achieve the effect of improving the ability to inhibit hydroxyl free radicals and improving the phagocytosis index

Active Publication Date: 2011-11-23
GUANGZHOU WEIRUTANG NUTRITION & HEALTH CONSULTING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the phosphorylation modification mainly focuses on starch, chitosan and cellulose, etc., and the research on the phosphorylation modification of exopolysaccharides of lactic acid bacteria has not been reported so far.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A preparation method of functional lactic acid bacteria exopolysaccharide, specifically comprising the following steps:

[0054] (1) Preparation of extracellular crude polysaccharides from lactic acid bacteria:

[0055] Set the viable count to 10 7 Each / mL Lactococcus lactis subsp. lactis strain starter (purchased from China Industrial Microbiology Culture Collection Management Center) was inoculated on the improved BLX medium at an inoculation amount of 3.0% (v / v), and firstly incubated at 37°C. Cultivate at 28°C for 15 hours, centrifuge at 6000-7000rpm for 15-20min to remove the bacterial cells to obtain a fermentation broth containing extracellular polysaccharides, and then use an ultrafiltration device to filter the extracellular polysaccharides in the fermentation broth of Lactococcus lactis subsp. The polysaccharide is concentrated by ultrafiltration, and then 10% trichloroacetic acid is added to the concentrated solution to precipitate TCA precipitation, 0-4 o ...

Embodiment 2

[0066] With embodiment 1, its difference is: in the preparation step of the extracellular crude polysaccharide of lactic acid bacteria, Lactococcus lactis subsp. 6 each / mL and 4.0% (v / v); cultured at 37°C for 20 h, and then at 30°C for 12 h; during the phosphorylation reaction, the phosphate used was sodium tripolyphosphate, powdered crude polysaccharide and Sodium tripolyphosphate (dissolved in a small amount of water first) was mixed at a mass ratio of 6:2, adjusted to pH 6.0, freeze-dried, and heated at 80°C for 5 hours; the selenizing agent used in the selenization reaction was selenium acetate Ether, heated at 45 ° C for 15h.

Embodiment 3

[0068] With embodiment 1, its difference is: in the preparation step of the extracellular crude polysaccharide of lactic acid bacteria, Lactococcus lactis subsp. 5each / mL and 5.0% (v / v); first cultured at 37°C for 20 h, then at 32°C for 10 h; during the phosphorylation reaction, the phosphate used was sodium pyrophosphate, powdered crude polysaccharide and three Sodium polyphosphate (dissolved in a small amount of water first) was mixed at a mass ratio of 6:3, and the pH was adjusted to 4.0, freeze-dried, and heated at 90°C for 4 hours; the selenizing agent used in the selenization reaction was selenyl ether acetate And the mixture of sodium selenite, heated at 55 ° C for 12h.

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Abstract

The invention discloses a method for preparing functional extracellular polysaccharide of lactic acid bacteria, which is characterized by comprising the following steps of: inoculating a Lactococcus lactis subsp. lactis strain fermentation agent for culture, performing ultrafiltration concentration, precipitation and ethanol extraction on fermentation liquor, centrifuging a precipitate, and performing freeze drying to obtain powdery crude extracellular polysaccharide; performing separation and purification by using a diethylaminoethanol (DEAE) cellulose ion exchange column and SepharoseCL-6B gel column chromatography in turn, desalting, performing ultrafiltration concentration, precipitating by using an ethanol solution, and performing freeze drying on a precipitate to obtain pure extracellular polysaccharide; and performing phosphorylation and selenide formation on the pure extracellular polysaccharide. The method has the advantages that: the extracellular polysaccharide produced by the Lactococcus lactis subsp. lactis strain is subjected to selenide formation and phosphorylation modification by a dry heating method, and the functional extracellular polysaccharide of lactic acid bacteria with obvious antioxidant and immunity enhancing effects is obtained.

Description

technical field [0001] The invention relates to a microbial exopolysaccharide, in particular to a preparation method of a functional lactic acid bacteria exopolysaccharide. Background technique [0002] In recent decades, due to the special advantages of microbial exopolysaccharides in product structure, performance and production, they have been vigorously researched and developed, and the development of microbial exopolysaccharides has become one of the hotspots in industrial microbiology research. Exopolysaccharide (EPS) of lactic acid bacteria is a mucus polysaccharide or capsular polysaccharide secreted outside the cell wall during the growth and metabolism of lactic acid bacteria. They have the functions of thickening, emulsifying, moisturizing and stabilizing, and help improve the flow of fermented dairy products Variable chemical properties, reduce whey precipitation, improve the texture and sensory quality of fermented milk, some lactic acid bacteria exopolysacchari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00A61P39/06A61P37/04C12R1/01
Inventor 潘道东曾小群曹锦轩
Owner GUANGZHOU WEIRUTANG NUTRITION & HEALTH CONSULTING CO LTD
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