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Nano selenium micromolecular microbial polysaccharide as well as preparation method and application thereof

A microbial polysaccharide and small molecule technology, applied in application, food preparation, food science and other directions, can solve the problems of low polysaccharide yield and complex modification process, and achieve the effect of strong anti-tumor activity, strong competitive advantage and stable quality

Inactive Publication Date: 2012-08-22
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] To sum up, there are many polysaccharides developed and reported at present, most of which are fungal polysaccharides, which are obviously limited by resources; bacterial polysaccharides with biological activity are less, but the yield is higher than that of fungal polysaccharides; Small molecule modification and modification have become research hotspots in the field of polysaccharides, and some modified polysaccharide products have also been developed, but most of them have defects such as low polysaccharide yield and complicated modification process.

Method used

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  • Nano selenium micromolecular microbial polysaccharide as well as preparation method and application thereof
  • Nano selenium micromolecular microbial polysaccharide as well as preparation method and application thereof
  • Nano selenium micromolecular microbial polysaccharide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Take LREPS with a molecular weight of 11.4KD, and prepare a 6mg / mL LREPS solution with ultrapure water; at the same time, prepare a 60mg / mL Vc solution and a 60μg / mL SeO 2 the solution

[0031] (2) Mix 20mL of the above-mentioned LREPS solution and 2mL of the Vc solution, stir magnetically for 30min to fully mix into a homogeneous system.

[0032] (3) 48mL SeO 2 The solution was added dropwise into the above homogeneous system using a vacuum dropping funnel, stirred while dropping, and 50 mL of ultrapure water was added.

[0033] (4) The above system was reacted for 5 minutes under the ultrasonic state.

[0034] (5) The reaction system was dialyzed with ultrapure water for 30 hours, and it was detected that selenium was no longer precipitated in the dialyzed fluid.

[0035] (6) Freeze drying to obtain Nano-Se-LREPS, the selenium content of which is 16.2 μg / mg, and the average particle diameter of selenium particles is 380 nm. Nano-selenium small-molecule microbi...

Embodiment 2

[0037] (1) Take LREPS with a molecular weight of about 11.0KD, and prepare a 6mg / mL LREPS solution with ultrapure water; at the same time, prepare a 60mg / mL Vc solution and a 60μg / mL SeO 2 the solution

[0038] (2) Mix 20mL of the above-mentioned LREPS solution and 1.6mL of Vc solution, and stir magnetically for 30min to fully mix into a homogeneous system.

[0039] (3) 32mL SeO 2 The solution was added dropwise into the above homogeneous system using a vacuum dropping funnel, stirred while dropping, and 50 mL of ultrapure water was added.

[0040] (4) The above system was reacted for 7 minutes in the ultrasonic state.

[0041] (5) Dialyze the reaction system with ultrapure water until it is detected that there is no selenium in the dialyzed fluid.

[0042] (6) Freeze drying to obtain Nano-Se-LREPS, the selenium content of which is 15.2 μg / mg, and the average particle diameter of selenium particles is 370 nm.

Embodiment 3

[0044] (1) Take LREPS with a molecular weight of about 10.2KD, and prepare a 6mg / mL LREPS solution with ultrapure water; at the same time, prepare a 60mg / mL Vc solution and a 60μg / mL SeO 2 solution.

[0045] (2) Mix 10 mL of the above-mentioned LREPS solution and 1.2 mL of Vc solution, and stir magnetically for 30 minutes to fully mix them into a homogeneous system.

[0046] (3) 20mL SeO 2 The solution was added dropwise to the above homogeneous system using a vacuum dropping funnel, stirred while dropping, and 25 mL of pure water was added.

[0047] (4) The above system was reacted for 10 minutes under ultrasonic state.

[0048] (5) Dialyze the reaction system with ultrapure water until the Vc detects that there is no selenium in the dialyzed fluid.

[0049] (6) Freeze drying to obtain Nano-Se-LREPS, the selenium content of which is 16.4 μg / mg, and the average particle diameter of selenium particles is 380 nm.

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Abstract

The invention provides a nano selenium micromolecular microbial polysaccharide, which is prepared under ultrasonic condition by taking micromolecular Caragana rhizobia extracellular polysaccharide as a raw material through uniformly dispersing and co-acting with selenium dioxide and a reducing agent. The production method specifically comprises the steps of: preparing a micromolecular bacterial polysaccharide solution with a proper concentration, uniformly mixing micromolecular Caragana rhizobia polysaccharide with vitamin C, slowly dropwise adding selenium dioxide to the mixed system, carrying out ultrasonic dispersion and reaction, dialyzing and freeze-drying. The nano selenium micromolecular microbial polysaccharide product prepared by the preparing process has the selenium content up to 15.2-16.4mu g / mg, selenium average grain size about of 380 nm and polysaccharide yield more than70%. Mouse test shows that the nano selenium micromolecular polysaccharide has excellent anticancer activity, and can be applied to preparing anticancer medicaments and health products.

Description

technical field [0001] The invention relates to a method of physical dispersion and modification to prepare new polysaccharide derivatives with higher activity by treating bacterial polysaccharides obtained through microbial fermentation and small molecule transformation, specifically belonging to a nano-selenium small-molecule microbial polysaccharide and its production Methods and applications. Background technique [0002] In recent years, microbial polysaccharides, as a class of biologically active substances with great development potential, have attracted worldwide attention. From ganoderma lucidum polysaccharides and lentinan belonging to fungi to xanthan gum and lactic acid bacteria exopolysaccharides belonging to bacteria, etc., many polysaccharides have been developed. A microbial polysaccharide product. Among them, many polysaccharide products are used as food additives, thickeners, suspending agents and chemical coagulants, lubricants, flocculants, etc., and are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P35/00A61P37/04A23L1/09
Inventor 吕利华赵良启魏国琴
Owner SHANXI UNIV
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