Method for producing polysaccharides by fermenting yellow serofluid with edible and medicinal fungi

A technology of medicinal fungus and yellow pulp water, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of being unable to recycle, can only be discharged at will, and the unit content is low, so as to achieve high promotion and utilization Value, production cost reduction, high yield effect

Inactive Publication Date: 2010-09-15
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to produce a large amount of yellow pulp water in the process of traditional soy products, although it contains rich types of nutrients, but the unit content is very low, resulting in too high cost of activated carbon, membrane separation and other methods, which cannot be recycled. It can only be discharged arbitrarily, pollutes the environment seriously, etc., and provides a simple process, low cost, high nutrient recovery rate, which can not only make full use of yellow pulp water resources, but also reduce environmental pollution by using edible and medicinal fungal fermentation Method for producing polysaccharide from yellow pulp water

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: (the method utilizing mushroom (135) bacterial classification to produce fungal polysaccharide)

[0025] Follow these steps:

[0026] (1) Pretreatment of the yellow pulp water: the yellow pulp water produced during the processing of soybean products is concentrated with a Sehan brand 0.2MPa reverse osmosis membrane until the solid content reaches 30%, and then set aside;

[0027] (2) Preparation of culture medium: including,

[0028] 1) Seed medium: glucose 1.5%, K 2 HPO 4 0.5%, MgSO 4 0.2%, make up to 100% with pretreated yellow pulp water, pH 6, sterilized, and set aside;

[0029] 2) Production medium: glucose 1.5%, K 2 HPO 4 0.5%, MgSO 4 0.2%, make up to 100% with pretreated yellow pulp water, pH 6, sterilized, and set aside;

[0030] (3) Strain activation: Inoculate the cultivated strains of shiitake mushrooms on the PDA slant medium, cultivate them at 25-28°C for 10 days, store them in a refrigerator at 4°C, and store them for later use; ...

Embodiment 2

[0036] Embodiment 2: (the method that utilizes oyster mushroom (No. brown level 1) bacterial classification to produce fungal polysaccharide)

[0037] In this example, step (2) 2) production medium formula is: sucrose 0.5%, K 2 HPO 4 0.4%, MgSO 40.05%; Step (3) bacterial classification activation: Pleurotus ostreatus cultivated bacterial classification is inoculated on the PDA slant medium, cultivates 9 days in 25-28 ℃; The inoculation cultivation of step (4) seed liquid: the activated bacterial classification inoculation amount is 2%; step (5) inoculation culture of production medium: shake culture at 25-28°C for 7 days; step (6) extract fungal polysaccharides from mycelium: ultrasonic extraction time is 60 minutes, and the temperature is raised to 95 After leaching for 60 minutes at ℃; step (7) extracting fungal polysaccharides from the fermentation filtrate: the ultrasonic extraction time of the fermentation filtrate is 20 minutes; the remaining process steps are the sam...

Embodiment 3

[0039] Embodiment 3: (the method utilizing Hericium erinaceus (Su monkey 19) bacterial classification to produce fungal polysaccharide)

[0040] In this example, step (2) 2) production medium formula is: brown sugar 0.8%, K 2 HPO 4 0.3%, MgSO 4 0.08%; step (3) bacterial classification activation: Hericium erinaceus cultivation bacterial classification is inoculated on the PDA slant medium, cultivates 8 days in 25-28 ℃; The inoculation cultivation of step (4) seed liquid: activation bacterial classification inoculum 3%; step (5) inoculation culture of production medium: shake culture at 25-28°C for 7 days; step (6) extract fungal polysaccharides from mycelium: ultrasonic extraction time is 40 minutes, and the temperature is raised to Extract at 100°C for 60 minutes; step (7) extract fungal polysaccharides from the fermentation filtrate: the ultrasonic extraction time of the fermentation filtrate is 25 minutes; the remaining process steps are the same as in Example 1;

[00...

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PUM

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Abstract

The invention relates to a method for producing polysaccharides by fermenting yellow serofluid with edible and medicinal fungi, which belongs to the technical field of comprehensive utilization of biological extraction. The method comprises the following steps: preprocessing yellow serofluid; preparing the culture medium; inoculating and culturing the seed liquid and the production culture medium; extracting fungus intracellular polysaccharides from mycelia; and extracting fungus extracellular polysaccharides from the fermentation filtrate, and the like. In the invention, waste yellow serofluid generated in the production process of bean products is used as the basic raw material, and the method of submerged fermentation of the edible or medicinal fungi is used, thus the nutritional components are maximally biotransformed. The invention has the advantages of low investment, low material cost, simple operation, high polysaccharide yield and the like, reduces the environmental pollution and lowers the production cost by about 50%. The invention can be popularized in bean product and biochemical enterprises.

Description

technical field [0001] The invention relates to the technical field of biological extraction and comprehensive utilization, in particular to a method for producing and preparing active polysaccharides by using edible and medicinal fungi to ferment yellow pulp water. Background technique [0002] The annual consumption of soybean products in my country is close to 9 million tons, and a large amount of wastewater will be generated during the processing of soybean products, mainly yellow pulp water, the total amount of which is 5.5-7 times the weight of soybeans. Yellow pulp water is a kind of wastewater with high concentration and high degree of biodegradability, its COD and BOD are as high as 15200mg / L and 7700mg / L respectively. Due to the small scale of general soybean product enterprises, lack of capital and technical strength, and insufficient attention to the recycling of functional factors and environmental pollution, a large amount of soybean yellow pulp water is direct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 范雷法张作法吕国英潘慧娟
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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