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Human cancer maker detection kit and detection method

A technology for detection kits and detection methods, applied in measuring devices, biomaterial analysis, instruments, etc., can solve problems such as inability to identify and type at one time, achieve effective clinical diagnosis, enrich test results, and assist clinical diagnosis

Inactive Publication Date: 2017-06-23
江苏博铼生技医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Currently commercially available test kits and methods for human cancer markers include magnetic particle chemiluminescence immunoassay, time-resolved fluorescence immunoassay (TRFIA), coded suspension microchip method, immunochromatography method and mass spectrometry method, etc. There are still shortcomings that need to be improved, especially the detection can be carried out to know whether it is present, but it is impossible to identify all the types at one time, and the information that can assist doctors in diagnosis is limited in clinical practice.
In addition, most of the existing technologies are still machine-read test results, which cannot be combined with image recognition systems to read and analyze with software systems to obtain complete information on test results and reagent groups

Method used

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  • Human cancer maker detection kit and detection method
  • Human cancer maker detection kit and detection method
  • Human cancer maker detection kit and detection method

Examples

Experimental program
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Embodiment 1

[0079] The detection kit in Example 1 can simultaneously detect six human cancer markers, including carcinoembryonic antigen, alpha-fetoprotein, prostate specific antigen, tumor antigen 19-9, tumor antigen 125, and tumor antigen 15-3. Correspondingly, the microcarrier reagent contains six corresponding microcarriers, and each microcarrier is coated with a corresponding coating antibody. There are also six kinds of detection antibodies. The details of coating antibody and detection antibody are shown in Table 2 below. The standard solution is MarkerPlus Control (supplier: Bio-rad, catalog number: Cat No. 367), which contains the above six human cancer markers.

[0080] Table 2 Coating antibody and detection antibody

[0081]

[0082] The coating antibody and detection antibody screened in Example 1 can bind to the characteristic sites of the corresponding cancer markers, and the coating antibodies or detection antibodies corresponding to different cancer markers will not...

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PUM

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Abstract

The invention relates to a human cancer marker detection kit and a detection method. The detection kit includes microcarriers, coating antibodies and detection antibodies. There is at least one kind of microcarrier, and the microcarrier has coding information, and the coding information of microcarriers for different human cancer markers is different. Coating antibodies are used to coat microcarriers. Both the coating antibody and the detection antibody are selected from the antibodies of carcinoembryonic antigen, alpha-fetoprotein, prostate specific antigen, tumor antigen 19-9, tumor antigen 125 and tumor antigen 15-3 For at least one of the corresponding human cancer markers, the coating antibody and the detection antibody have different antigen binding sites. The detection kit can be used to detect up to six human cancer markers, thereby enriching the detection results and assisting clinical diagnosis more effectively.

Description

technical field [0001] The invention relates to the field of cancer marker detection, in particular to a human cancer marker detection kit and detection method. Background technique [0002] Cancer markers are usually glycoproteins, which are produced and secreted by specific cancer cells during growth, or produced by affecting adjacent normal cells. Generally, the appearance of cancer markers can be detected in the patient's blood, urine, or specific tissues. Therefore, detection of changes in cancer markers in the body can be used as an auxiliary tool for clinically identifying tumors. When cancer cells are active in the body, the concentration of cancer markers will increase, but if there are other physiological factors or diseases, the increase of such substances may also be caused. Therefore, clinically detected changes in the value of cancer markers are not necessarily You have cancer, and normal values ​​do not mean you are healthy, so the test results of human cance...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57484G01N33/57473G01N33/57476G01N2333/47
Inventor 曹汀吴沁纭陈昶华黄勤修
Owner 江苏博铼生技医疗科技有限公司
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