Application of DKK1 as diagnostic marker

A use and technology of diagnostic reagents, applied in the field of DKK1 as a diagnostic marker, can solve the problem of unclear diagnostic ability of hepatocellular carcinoma or small hepatocellular carcinoma

Inactive Publication Date: 2014-01-01
SHANGHAI INST OF ONCOLOGY
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the current clinical significance of serum DKK1 for hepatocellular carcinoma, especially its ability to diagnose alpha-fetoprotein-negative hepatocellular carcinoma, the ability to differen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of DKK1 as diagnostic marker
  • Application of DKK1 as diagnostic marker
  • Application of DKK1 as diagnostic marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0091] As an embodiment of the present invention, the reagent is an anti-DKK1 antibody; more specifically, it is a monoclonal antibody or a polyclonal antibody.

[0092] Antibodies of the present invention can be prepared by various techniques known to those skilled in the art. For example, purified antigen can be administered to animals such as rabbits, mice, rats, etc. to induce polyclonal antibody production. Various adjuvants can be used to enhance the immune response, including but not limited to Freund's adjuvant and the like.

[0093] Antibodies of the invention may also be monoclonal antibodies. Such monoclonal antibodies can be prepared using hybridoma technology.

[0094] The antibody can be used in immunochemical techniques to detect the level of DKK1 in the specimen, so as to diagnose hepatocellular carcinoma, especially alpha-fetoprotein-negative hepatocellular carcinoma, early hepatocellular carcinoma or small hepatocellular carcinoma, or to judge the risk of d...

Embodiment 1

[0171] Example 1, Comparative analysis of the expression of DKK1 and AFP in non-cancerous liver tissue and hepatocellular carcinoma tissue and corresponding serum

[0172] The present inventors compared and analyzed DKK1 and AFP in 12 cases of non-cancerous liver tissues (4 cases were normal liver tissues, 8 cases were liver cirrhosis tissues) and 16 cases of hepatocellular carcinoma liver tissues (wherein 8 cases of patients had serum AFP≤20ng / ml, and the expression in serum AFP>20ng / ml of the other 8 cases. The results of semi-quantitative reverse transcription polymerase chain reaction (semi-qPCR) and real-time quantitative polymerase chain reaction (qRT-PCR) experiments showed that, compared with AFP, DKK1 was more active in 4 healthy controls (HC) and 8 In cases of liver cirrhosis (liver corrosion, LC), the expression was almost undetectable, but it was highly expressed in most hepatocellular carcinoma tissues (figure 1 A-B, 1D-E).

[0173] Enzyme-linked immunosorbent a...

Embodiment 2

[0175] Example 2. Serum DKK1 is a serum protein marker for the diagnosis of hepatocellular carcinoma

[0176] The inventor according to figure 2 To evaluate the diagnostic ability of serum DKK1 protein in AFP-negative HCC.

[0177] First, the inventors analyzed the expression of serum DKK1 and AFP in each group of the test set. Such as image 3 As shown in A and Table 7, the serum DKK1 protein level of the hepatocellular carcinoma (hepatocellular carcinoma, HCC) group was significantly higher than that of the normal control group (healthy controls, HC), chronic hepatitis B (chronic hepatitis B, CHB) control group and liver In the liver cirrhosis (LC) control group (P image 3 B). The concentrations of serum DKK1 and AFP in each group are shown in Table 8.

[0178] Table 7, the levels of serum DKK1 protein and serum AFP protein in each group in the test set and verification set

[0179]

[0180] Abbreviations: DKK1: dickkopf-1; AFP: alpha-fetoprotein, alpha-fetoprotein;...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses an application of DDK1 as a diagnostic marker. Through analysis of a large amount of hepatocellular carcinoma samples, the inventor analyzes the expressions of DKK1 and AFP, firstly discovers that serum DKK1 has a high sensitivity and specificity on diagnosis of hepatocellular carcinoma, especially diagnosis of alpha fetal protein negative hepatocellular carcinoma, identification of hepatocellular carcinoma and alpha fetal protein positive chronic liver disease, and/or diagnosis of hepatocellular carcinoma in the early stage or small hepatocellular carcinoma, and more especially the precision of clinical diagnosis can be largely increased by DKK1-AFP combined diagnosis.

Description

technical field [0001] The present invention belongs to the field of biomedicine; more specifically, the present invention relates to the use of DKK1 as a diagnostic marker, which can be used for: diagnosing alpha-fetoprotein-negative hepatocellular carcinoma, differentiating hepatocellular carcinoma and alpha-fetoprotein-positive chronic liver disease and / or diagnosing Early-stage hepatocellular carcinoma or small hepatocellular carcinoma. Background technique [0002] Primary liver tumors include hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma, mixed hepatocellular carcinoma, cystadenoma of the bile duct, hepatoblastoma, hemangioma, carcinoid, lymphoma, epithelioid vascular endothelium Tumor, squamous cell carcinoma, teratoma, and leiomyosarcoma, fibrosarcoma, malignant fibrous histiocytoma, rhabdomyosarcoma and other liver sarcomas. Hepatocellular carcinoma (HCC) is one of the histological subtypes. Hepatocellular carcinoma (HCC) is one of the most commo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/574
CPCC12Q1/6886G01N33/57438C07K14/47G01N33/574C12Q2600/158C07K14/4715G01N2333/4703G01N33/57484
Inventor 覃文新沈秋瑾顾健人杨胜利
Owner SHANGHAI INST OF ONCOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap