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Method for detecting alpha-fetal protein on basis of RGO-CS-Fc/Au NPs nano composite material and aptamers

A technology of nanocomposite materials and alpha-fetoprotein, applied in the field of biological detection, can solve the problems of complex operation, time-consuming technical requirements, expensive instruments, etc., and achieve the effect of small background interference

Active Publication Date: 2019-08-20
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

The instruments used in these methods are expensive, complicated to operate, time-consuming and technically demanding. It is necessary to establish a fast, sensitive and easy-to-operate AFP detection method.

Method used

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  • Method for detecting alpha-fetal protein on basis of RGO-CS-Fc/Au NPs nano composite material and aptamers
  • Method for detecting alpha-fetal protein on basis of RGO-CS-Fc/Au NPs nano composite material and aptamers
  • Method for detecting alpha-fetal protein on basis of RGO-CS-Fc/Au NPs nano composite material and aptamers

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Embodiment Construction

[0045] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0046] A method for detecting alpha-fetoprotein based on RGO-CS-Fc / Au NPs nanocomposite combined with a suitable ligand, the detection principle is shown in figure 1 . RGO-CS-Fc / Au NPs were modified on the surface of screen-printed electrodes by electrodeposition technology and electrostatic adsorption. The AFP aptamer was loaded on the surface of RGO-CS-Fc / Au NPs material through nanotechnology and intermolecular force, and the aptamer existed on the biosensing interface in the form of a single-chain structure due to its unstable spatial structure. After adding AFP to the biosensing interface, AFP can specifically bind to the AFP aptamer to generate a stable spatial structure, which can be arranged in an orderly manner on the electrode surface. The electrochemical signal in the PBS solution (0.2 mol / L, pH6.0) before and after AFP was detec...

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Abstract

The invention relates to a method for detecting alpha-fetal protein on the basis of an RGO-CS-Fc / Au NPs nano composite material and aptamers. According to the method, the surface of a silk-screen printing electrode is modified with RGO-CS-Fc / Au NPs by means of an electro-deposition technology and an electrostatic adsorption effect; AFP aptamers are loaded on the surface of the RGO-CS-Fc / Au NPs bymeans of a nanotechnology and an intermolecular force, and the aptamers exist on the surface of the composite material in the form of single-chain structures due to the unstable spatial structures ofthe aptamers; after AFP is added into the surface of the electrode, the AFP can be specifically combined with the AFP aptamers, so that stable spatial structures can be generated, and therefore, the AFP and the aptamers can be orderly arranged on the surface of the electrode; a current value is detected through a DPV method, a relation curve of current and the concentration of the alpha-alpha protein is depicted; and therefore, the quantitative detection of the alpha-alpha protein is realized. The method has the advantages of simple operation, time-saving performance, low cost and low detection limit.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a method for detecting alpha-fetoprotein based on a nanocomposite material combined with a suitable ligand. Background technique [0002] Alpha-fetoprotein (AFP) is an acidic glycoprotein belonging to the albumin family, which is a single polypeptide chain formed by more than 500 amino acid residues. For normal adults, the content of AFP in the human body is extremely low. AFP detection methods mainly include radioimmunoassay, fluorescence immunoassay, enzyme-linked immunosorbent assay, chemiluminescence immunoassay, flow immunoassay, electrochemical immunosensor, piezoelectric immunosensor and polypeptide complex protein microarray, etc. The invention patent with publication number CN 104677889 B relates to a method for detecting alpha-fetoprotein based on a luminol-functionalized magnetic immunoprobe. Luminol-functionalized magnetic microspheres were used to la...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/48G01N27/3272G01N27/3277G01N27/3278
Inventor 李桂银李文湛周治德梁晋涛
Owner GUILIN UNIV OF ELECTRONIC TECH
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