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Combined general check protein chip for early-stage cancers mainly comprising lung cancer

A protein chip and cancer technology, applied in the field of protein chips, can solve the problems of inability to do various common types of cancer, census, and low detection efficiency of lung cancer, and achieve the effects of saving national financial and material resources, good repeatability, and improving labor efficiency

Active Publication Date: 2012-12-26
马鞍山微因泰克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the disadvantages of low detection efficiency of the protein chip for lung cancer in the prior art and the inability to carry out general surveys of various common types of cancers, and provide a protein chip for joint screening of early cancers mainly based on lung cancer. The protein chip invented by the invention can simultaneously detect whether there are multiple cancer symptoms such as lung cancer in multiple subjects through one detection and analysis. High, reliable, can be used as the basis for clinical diagnosis

Method used

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  • Combined general check protein chip for early-stage cancers mainly comprising lung cancer
  • Combined general check protein chip for early-stage cancers mainly comprising lung cancer
  • Combined general check protein chip for early-stage cancers mainly comprising lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] combine figure 1 , the protein chip of this embodiment is suitable for early screening of lung cancer, including a film base and marker antibodies distributed on the film base, the film base of this embodiment is a FAST protein chip film base 11 with a three-dimensional nitrocellulose matrix, the protein chip The detection efficiency of the technology depends on the stability of the protein and the carrier plane. The cancer markers of the early lung cancer screening protein chip are difficult to maintain their biological activity, and the fixation efficiency on the traditional chip carrier is low and unstable, which limits this kind of The detection efficiency of the chip, the three-dimensional nitrocellulose matrix is ​​a three-dimensional structure, which can produce irreversible non-covalent binding with proteins, and the three-dimensional nitrocellulose structure has a wider threshold for protein signal reception intensity from an optical point of view , so that th...

Embodiment 2

[0034] combine figure 2 , this embodiment is based on lung cancer-based early cancer joint census protein chip, including a substrate and marker antibodies distributed on the substrate, the substrate of this embodiment is a FAST protein chip substrate with a three-dimensional nitrocellulose matrix 11 , the FAST protein chip base 11 is a cuboid structure, and the FAST protein chip base 11 is provided with 16 spotting modules 12, and each spotting module 12 is suitable for a subject, and the 16 spotting modules 12 are divided into There are two columns, each column is 8 spotting modules 12, and seven rows and six columns of circular spotting spot grooves 13 are arranged on the spotting module 12, and the following marker antibodies are fixed in the spotting spot grooves 13: 14- 3-3θ, LAMR-1, TSGF, CEA, SCC, CYFRA21-1, AFP, CA242, MG-Ag, CA199, TPA, EA-IgA and positive control, from top to bottom, from left to right in this example Number, positive control 1, 14-3-3θ was fixed ...

Embodiment 3

[0038] combine image 3 , this embodiment is to expand the joint census of early cancers that are specific to men and women, mainly lung cancer. The protein chip includes a film base and marker antibodies distributed on the film base. The film base of this embodiment is FAST with a three-dimensional nitrocellulose matrix. The protein chip base 11, the FAST protein chip base 11 is a rectangular parallelepiped structure, the FAST protein chip base 11 is provided with 16 spotting modules 12, each spotting module 12 is suitable for a subject, the 16 points The sample module 12 is divided into two columns, and each column is 8 sample spot modules 12. The spot sample module 12 is provided with six rows and six columns of circular spot spot grooves 13, and the following symbols are fixed in the spot spot grooves 13 Antibodies: 14-3-3θ, LAMR-1, TSGF, CEA, SCC, AFP, CA242, MG-Ag, CA199, TPA, EA-IgA, CA125, CYFRA21-1, CA153, SF, PSA and positive control, Counting from top to bottom and...

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Abstract

The invention discloses a combined general check protein chip for early-stage cancers mainly comprising lung cancer and belongs to the technical field of a protein chip. The combined general check protein chip for the early-stage cancers mainly comprising the lung cancer, provided by the invention, comprises a chip substrate and marker antibodies distributed on the chip substrate, wherein the chip substrate is an FAST protein chip substrate having a three-dimensional nitrocellulose basic material; one or more sample application module is arranged on the FAST protein chip substrate; the following marker antibodies are fixed on the sample application module: 14-3-3 theta, LAMR-1, TSGF (Tumor Specific Growth Factor), CEA (Cancer Embryo Antigen), SCC (Squamous Cell Carcinoma), CYFRA21-1 and positive control; the following marker antibodies are also fixed: AFP (Alpha Fetal Protein), CA242, MG-Ag, CA199, TPA (Tissue Polypeptide Antigen) and EA-IgA; and the following marker antibodies are further fixed: CA125, CA153, SF (Serum Ferritin) and PSA (Prostate Specific Antigen). According to the combined general check protein chip provided by the invention, the related indexes are highly integrated; the combined general check protein chip has the advantages of excellent stability, strong specificity, long retention period, high sensitivity, excellent repeatability, convenience in operation and low cost; the labor efficiency is increased; and a cancer patient is diagnosed and treated from the general check before clinical symptoms occur.

Description

technical field [0001] The invention relates to the technical field of protein chips, and more specifically, relates to a protein chip for early cancer joint census mainly involving lung cancer. Background technique [0002] In recent years, malignant tumors have become one of the major diseases that seriously affect human health and threaten human life, and the cost of treatment is high. However, malignant tumors are not incurable. Early detection and diagnosis are of great importance to reduce patient mortality and improve treatment effects. significance. As long as early detection and early treatment, the cure rate can reach more than 90%, and the cost of early treatment is relatively low. With the development of medical science, great progress has been made in the early diagnosis of malignant tumors. Modern medicine has many methods for the early diagnosis of malignant tumors, including imaging examination, endoscopy, pathological examination, serological examination, ...

Claims

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Application Information

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IPC IPC(8): G01N33/574
Inventor 阮俞敏子张荔
Owner 马鞍山微因泰克生物科技有限公司
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