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AFP (alpha fetal protein) detection kit

A detection kit and detection line technology, applied in the field of alpha-fetoprotein detection kit and its preparation, can solve the problems of alpha-fetoprotein accuracy, sensitivity, poor repeatability, unsuitable for clinical use, cumbersome operation, etc., and achieve high Sensitivity, high specificity, quick and easy operation

Active Publication Date: 2015-02-18
ZYBIO INC
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are still many defects in the currently known detection methods of alpha-fetoprotein (AFP), such as complicated operation and long time-consuming, which are not suitable for clinical use
At present, the accuracy, sensitivity and repeatability of alpha-fetoprotein (AFP) detection kits on the Chinese market are still poor

Method used

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Embodiment 1

[0044] Preparation of test paper card of the present invention:

[0045] 1) Use the pretreatment buffer to pretreat the gold standard pad. The pretreatment buffer is: stachyose 2g / L, alum 0.5g / L, fructose sodium diphosphate 1.5g / L, sodium hexametaphosphate 0.3g / L L, glycine 1.88g / L aqueous solution, pH=7.4, the specific steps of pretreatment are: soak the gold standard pad in the pretreatment solution for 2 hours, take it out and dry it at 37°C; The protein AFP antibody solution was sprayed on the pretreated gold label pad to obtain a gold label pad coated with the alpha-fetoprotein AFP antibody. The mass ratio of the fluorescent microspheres to the antibody in the solution was 5:1, and the concentration of the solution was 10 mg / ml. The amount of spraying is 4ul / cm;

[0046] 2) Spray 1 mg / ml of alpha-fetoprotein AFP antibody solution and goat anti-mouse antibody solution on the detection line and quality control line of the nitrocellulose membrane respectively, and the spray...

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Abstract

The invention relates to the field of biological detection, and particularly relates to a detection kit for quantitatively detecting AFP (alpha fetal protein) and a preparation method and use of the detection kit. The invention provides a detection kit for quantitatively detecting AFP. The detection kit comprises a test paper card, wherein the test paper card comprises a bottom plate, and a sample pad, a gold-marking pad, a nitrocellulose membrane and an absorbent pad which are arranged on the surface of the bottom plate and are sequentially arranged from the loading end; the gold-marking pad comprises an AFP antibody; the nitrocellulose membrane is coated with a detection line and a quality control line; and the AFP antibody on the gold-marking pad is marked by virtue of fluorescent microspheres. The detection kit for quantitatively detecting the AFP, provided by the invention, is used for detecting the AFP through a fluorescent microsphere immune chromatography technology for the first time, has sensitivity and specificity, and has the advantages of being rapid and easy to operate, accurate in result, economical, applicable and the like.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to an alpha-fetoprotein detection kit and its preparation method and application. Background technique [0002] Alpha fetoprotein (AFP) is a glycoprotein synthesized by the liver and yolk sac in the early stage of fetal development. With the growth of fetal development and postnatal age, the level of AFP in the blood gradually decreases. The reference range of adult serum AFP concentration 1-20μg / L. AFP is a sensitive and specific marker of hepatocellular carcinoma (HCC). If the serum AFP concentration exceeds 400 μg / L for 4 weeks or 200-400 μg / L for more than 5 weeks, hepatocellular carcinoma is highly suspected; at the same time, the serum AFP level is still low. It can be used to judge the size of liver cancer, evaluate the curative effect and judge the prognosis of liver cancer. However, according to statistics, about 20%-30% of patients with HCC have low serum AFP levels. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/57438G01N33/68
Inventor 吉权
Owner ZYBIO INC
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