Alpha fetal protein (AFP) detection method based on fluorescent nano luminous and magnetic nano material
An alpha-fetoprotein antibody and nanoparticle technology, applied in the field of clinical detection, can solve the problems of low sensitivity, cumbersome operation, poor selectivity, etc., and achieve the effects of simplified detection means, high sensitivity and simple detection means
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[0035] Fe 3 o 4 - Preparation of Dextran:
[0036] 1. Weigh 1g of Dextran T40, dissolve in 2ml of ultrapure water, vacuumize for 20min, N 2 Saturation for 20min;
[0037] 2. Weigh 90mg of FeCl 3 ·6H 2 O and 154 mg FeSO 4 ·7H 2 O, dissolved in 3.4ml ultrapure water, vacuumed for 20min, N 2 Saturation for 20min;
[0038]3. Mix the products made in steps 1 and 2, put them in a 60°C water bath, stir with a magnet, add 0.6ml of concentrated ammonia water dropwise, and react for 15 minutes;
[0039] 4. Combined with magnetic separation, wash several times with ultrapure water.
[0040] Preparation of CdTe quantum dots:
[0041] 1. Vacuumize 40mL of ultrapure water for 20 minutes and then fill it with nitrogen for 20 minutes.
[0042] 2. Under magnetic stirring, sequentially add 2mL 5% Na-citrate solution, 4mL 0.04mol / L CdCl 2 2.5H 2 O solution, 4mL 0.01mol / L Na 2 TeO 3 Solution, 50mg L-cysteine and 50mg NaBH 4 .
[0043] 3. Magnetic stirring for 5 minutes to form ...
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