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Alpha fetal protein (AFP) detection method based on fluorescent nano luminous and magnetic nano material

An alpha-fetoprotein antibody and nanoparticle technology, applied in the field of clinical detection, can solve the problems of low sensitivity, cumbersome operation, poor selectivity, etc., and achieve the effects of simplified detection means, high sensitivity and simple detection means

Inactive Publication Date: 2009-05-27
DONGHUA UNIV
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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the shortcomings of background light interference, poor selectivity, cumbersome operation and low sensitivity in the determination of AFP by the existing immunoassay method, and provide a method using CdTe quantum dots and Fe 3 o 4 —A method for detecting AFP content in serum by Dextran nanoparticles

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  • Alpha fetal protein (AFP) detection method based on fluorescent nano luminous and magnetic nano material
  • Alpha fetal protein (AFP) detection method based on fluorescent nano luminous and magnetic nano material
  • Alpha fetal protein (AFP) detection method based on fluorescent nano luminous and magnetic nano material

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Embodiment 1

[0035] Fe 3 o 4 - Preparation of Dextran:

[0036] 1. Weigh 1g of Dextran T40, dissolve in 2ml of ultrapure water, vacuumize for 20min, N 2 Saturation for 20min;

[0037] 2. Weigh 90mg of FeCl 3 ·6H 2 O and 154 mg FeSO 4 ·7H 2 O, dissolved in 3.4ml ultrapure water, vacuumed for 20min, N 2 Saturation for 20min;

[0038]3. Mix the products made in steps 1 and 2, put them in a 60°C water bath, stir with a magnet, add 0.6ml of concentrated ammonia water dropwise, and react for 15 minutes;

[0039] 4. Combined with magnetic separation, wash several times with ultrapure water.

[0040] Preparation of CdTe quantum dots:

[0041] 1. Vacuumize 40mL of ultrapure water for 20 minutes and then fill it with nitrogen for 20 minutes.

[0042] 2. Under magnetic stirring, sequentially add 2mL 5% Na-citrate solution, 4mL 0.04mol / L CdCl 2 2.5H 2 O solution, 4mL 0.01mol / L Na 2 TeO 3 Solution, 50mg L-cysteine ​​and 50mg NaBH 4 .

[0043] 3. Magnetic stirring for 5 minutes to form ...

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Abstract

The invention relates to a method for determining the content of AFP in serum by using CdTe quantum dots and Fe3O4-Dextran nano particles, characterized by comprising the steps of (a) preparing Fe3O4-Dextran-first antibody complex; (b) preparing CdTe-second antibody complex; (c) drawing 'fluorescence intensity-AFP concentration' standard work curve, and calculating work equation; and (d) measuring the fluorescence intensity value for the work equation to determine the AFP content of the patents with liver cancer. The method is simple and has high sensitivity, can easily detect the disease for the early cancer patients so as to treat the patients in time, and furthermore, the method opens up a road for the clinic detection of liver cancer.

Description

technical field [0001] The invention relates to the field of clinical detection, in particular to a method using CdTe quantum dots and Fe 3 o 4 —A method for detecting AFP content in serum by Dextran nanoparticles. Background technique [0002] Primary liver cancer is one of the most common malignant tumors in my country, and its incidence is increasing year by year. It has become the second cancer killer in my country. Alpha-fetoprotein (AFP) is an embryonic protein, mainly produced by liver cells and yolk sac during the embryonic period, and its concentration drops rapidly after birth, approaching the adult level within a few months to a year <20ng / ml. AFP is a specific tumor marker for the diagnosis of primary liver cancer, and has the functions of establishing diagnosis, early diagnosis and differential diagnosis. It is generally believed that if AFP in serum is positive or quantified > 400 ng / ml, > 200 ng / ml for 8 weeks, and alanine aminotransferase is norma...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/533
Inventor 周兴平胡栩华倪似愚李凯李靖
Owner DONGHUA UNIV
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