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Preparation method of a label-free photoelectrochemical alpha-fetoprotein immunosensor

An immune sensor and photoelectrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of low sensitivity and unfavorable practical application of photoelectrochemical sensors, and achieve improved detection sensitivity, high selectivity detection, and operation convenient effect

Inactive Publication Date: 2015-10-21
UNIV OF JINAN
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitivity of photoelectrochemical sensors based on single semiconductor nanomaterials is generally not high, which is not conducive to practical applications

Method used

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  • Preparation method of a label-free photoelectrochemical alpha-fetoprotein immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of AuAg NRs

[0029] Mix 20 mL of gold nanorod sol Au NRs and 20 mL of 0.05 mol / L cetyltrimethylammonium bromide CTAB solution, stir at 30 °C, and sequentially add 3 mL of 0.1 mol / L L-ascorbic acid AA solution , 0.5 mL of 0.05 mol / L silver nitrate AgNO 3 solution and 3 mL of 0.1 mol / L sodium hydroxide NaOH solution, stop stirring, let stand for 1 h, centrifuge, and redisperse the product into 20 mL of water to obtain AuAg NRs;

[0030] The Au NRs is an aqueous solution of gold nanorods, the gold nanorods are rod-shaped gold nanoparticles, and the length of the gold nanorods is 20nm.

Embodiment 2

[0031] Example 2 Preparation of AuAg NRs

[0032] Mix 20 mL of Au NRs and 20 mL of 0.1 mol / L CTAB solution, stir at 40 °C, and sequentially add 5 mL of 0.1 mol / L AA solution, 0.7 mL of 0.05 mol / L AgNO 3 solution and 5 mL of 0.1 mol / L NaOH solution, stop stirring, let stand for 2h, centrifuge, and redisperse the product in 20mL of water to obtain AuAg NRs;

[0033] The Au NRs is an aqueous solution of gold nanorods, the gold nanorods are rod-shaped gold nanoparticles, and the length of the gold nanorods is 30nm.

Embodiment 3

[0034] Example 3 Preparation of AuAg NRs

[0035] Mix 20 mL of Au NRs and 20 mL of 0.2 mol / L CTAB solution, stir at 45 °C, and sequentially add 6 mL of 0.1 mol / L AA solution, 1 mL of 0.05 mol / L AgNO 3 solution and 6 mL of 0.1 mol / L NaOH solution, stop stirring, let it stand for 3 hours, centrifuge, and redisperse the product in 20mL of water to obtain AuAg NRs;

[0036] The Au NRs is an aqueous solution of gold nanorods, the gold nanorods are rod-shaped gold nanoparticles, and the length of the gold nanorods is 40nm.

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Abstract

The invention discloses a preparation method of an unlabelled photoelectrochemical alpha fetoprotein immunosensor, and belongs to the fields of novel nano functional materials and biosensors. On the basis of a titanium dioxide nano particle base, a trimetal alloy nano material in a dendrite nanorod form is prepared by a photoelectrochemical synthetic method, so that the prepared unlabelled photoelectrochemical alpha fetoprotein immunosensor for detecting alpha fetal protein is low in cost, high in sensitivity, good in specificity, fast in detection and simple to prepare.

Description

technical field [0001] The invention relates to a preparation method of a label-free photoelectrochemical alpha-fetoprotein immunosensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] The detection of tumor markers can reflect the biological characteristics of tumors, and is a key link in the clinical treatment and diagnosis of tumor patients. Alpha-fetoprotein is a tumor marker, which is used as a specific clinical indicator for the diagnosis of primary liver cancer. At present, the methods for detecting alpha-fetoprotein mainly include radioimmunoassay and mass spectrometry. Such methods require expensive instruments and complex operations, and laboratory personnel need professional training before they can perform detection. Therefore, it is of great significance to develop an alpha-fetoprotein sensor with high sensitivity, strong specificity and fast detection speed. [0003] Due to the character...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N27/26
CPCG01N27/26G01N33/689
Inventor 张勇庞雪辉魏琴胡丽华马洪敏范大伟
Owner UNIV OF JINAN
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