Magnetic particle chemiluminescence immune assay kit of tumor marker AFP (alpha fetal protein) and detection method thereof

A chemiluminescence immunoassay and detection method technology, which is applied in the field of magnetic particle chemiluminescence immunoassay kits, can solve the problems of insufficient sensitivity of ELISA detection, and achieve the effect of simple pretreatment process, high sensitivity, and convenient and easy detection method
CN104034892AInactive Publication Date: 2014-09-10GUANGXI DOCTOR HAIYI INFORMATION TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGXI DOCTOR HAIYI INFORMATION TECH
Publication Date
2014-09-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a magnetic particle chemiluminescence immune assay kit of tumor marker AFP (alpha fetal protein) and a detection method of the magnetic particle chemiluminescence immune assay kit, which belongs to the technical field of the immune detection and analysis. An AFP monoclonal antibody marked by fluorescein isothiocyanate (FITC) and a monoclonal antibody marked by alkaline phosphatase (AP) are combined with an antigen to form a sandwiched immune compound of an FITC marked antibody-antigen-AP marked antibody, and the sandwiched immune compound is similar to a sandwich structure. Then the magnetic particles which are connected with the anti-FITC antibody are added, the specific binding of the anti-FITC antibody and the FITC enables antigen-antibody complex to be connected onto the magnetic particles, the magnetic particles are directly precipitated in an external magnetic field, and the compound formed in the immune reaction manner can be separated from other non-combined substances without centrifuging. According to the kit, the chemiluminescence is combined with the magnetic particles, a reaction system which is approximate to the homogenous phase is provided, and compared with the prior art, the kit has the advantages of high sensitivity, wide linear range, high speed and the like; moreover, the product cost is greatly reduced, and the application prospect on the aspects such as the clinical inspection is promising.
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Description

technical field

[0001] The invention belongs to the technical field of immune detection and analysis, and relates to a magnetic particle chemiluminescent immunoassay kit for detecting the content of tumor marker AFP in serum and a detection method thereof. Background technique

[0002] Tumor markers (tumor mark, TM) refer to substances secreted or shed by tumor cells into body fluids or tissues, or substances produced by the host in response to parasites in the body and entered into body fluids or tissues. Most of the commonly used clinical tumor markers are non-specific markers. These markers also appear in benign tumors and normal tissues, but their levels are significantly increased when tumors occur. Since such markers are not tumor-specific, they are also called broad-spectrum tumor markers.

[0003] Alpha-fetoprotein (α-Fetoprotein, AFP) is a single multimer peptide bond glycoprotein, containing 590 amino acids, with a molecular weight of about 70KD, and belongs to th...

Claims

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