Glyphosate resistant EPSP synthetase GmEPSPS, and coding gene and application thereof

A glyphosate-resistant, coding technology that can be used in applications, genetic engineering, plant gene improvement, etc., and can solve problems such as low expression efficiency
CN102766609AInactive Publication Date: 2012-11-07CHONGQING ACAD OF AGRI SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHONGQING ACAD OF AGRI SCI
Publication Date
2012-11-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a glyphosate resistant protein, and a coding gene and application thereof. The protein provided by the invention is (a) or (b) or (c) as follows: (a) a protein composed of an amino acid sequence shown as 73rd-504th site of a sequence 1 in the sequence table; (b) a protein composed of the amino acid sequence shown in the sequence 1 in the sequence table; and (c) a protein, derived from the sequence 1 and with glyphosate resistance, obtained by substitution and / or deletion and / or addition of one or several amino acid residues on the amino acid sequence of (a) or (b). The GmEPSPS protein and the coding gene thereof provided by the present invention have significant resistance effect on a glyphosate herbicide. Experiments prove that GmEPSPS transgenic tobacco and GmEPSPS transgenic maize can still grow normally under 0.15% of glyphosate, but non-transgenic tobacco and maize are obviously suppressed in growth, and have leaves yellowing and withered. It shows that GmEPSPS can stably and efficiently express in plants, and be further used in production of glyphosate resistant transgenic plants.
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Description

Technical field

[0001] The invention belongs to the field of plant genetic engineering, and relates to a glyphosate-resistant EPSP synthase and its coding gene and application, and particularly relates to an artificially modified and synthesized glyphosate-resistant EPSP synthase and its coding gene GmEPSPS, and containing the gene The expression vector and its application in the development of herbicide-resistant transgenic plants. Background technique

[0002] Glyphosate is a systemic, non-selective, broad-spectrum biocidal herbicide with stable physical and chemical properties, high efficiency, low toxicity, low residue, easy to be decomposed by microorganisms, and no damage to the soil environment. It has been widely used in agriculture In production, it has become the largest pesticide variety in the world. The related mechanism of action is that glyphosate is an analog of phosphoenolpyruvate (PEP), so glyphosate, shikimate-3-phosphate, and EPSP synthase are easily combined...

Claims

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