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Production method of preparing corn peptide powder by means of bionic enzymatic hydrolysis

A biomimetic enzymatic hydrolysis and peptide powder technology, which is applied to the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of waste of resources, poor water solubility, etc., and achieve the effects of low cost, simple preparation process, and no toxic and side effects.

Inactive Publication Date: 2017-07-25
ANHUI BIOLOGICAL PEPTIDE IND RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of essential amino acids such as lysine and tryptophan in corn gluten meal, and its special taste and color, and poor water solubility, corn gluten meal is mainly used for roughage production or directly discharged, resulting in a huge waste of resources. waste

Method used

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  • Production method of preparing corn peptide powder by means of bionic enzymatic hydrolysis
  • Production method of preparing corn peptide powder by means of bionic enzymatic hydrolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 This embodiment comprises the following steps:

[0026] (1) Add 20 liters of purified water to the bionic enzymolysis tank, start the stirring paddle, set the rotating speed at 60 rpm, take 2 kg of corn gluten powder (corn protein isolate, protein content is more than 90%, and water content is less than 10%), slowly Add, continue to stir and swell for 30 minutes after the addition is complete, heat the interlayer to 80-85°C for 15 minutes to sterilize, pass cooling water to the interlayer to cool down to about 55°C in the tank, start the interlayer water and heater circulation, and the heater heats up Keep warm at 55°C;

[0027] (2) Start the pH detection terminal, add dilute hydrochloric acid to the bionic enzymolysis tank to adjust the pH value to 2.0 with a peristaltic pump, add 1% pepsin (enzyme activity: 3000U / g) of zein content, keep warm for 1 hour, and Monitor the pH value and add dilute hydrochloric acid to keep the pH value within the range of 1....

Embodiment 2

[0031] Embodiment 2 This embodiment comprises the following steps:

[0032] (1) Add 40 liters of purified water to the bionic enzymolysis tank, start the stirring paddle, set the speed at 30 rpm, take 2 kg of corn gluten powder, add it slowly, continue to stir and swell for 30 minutes after the addition, and heat the interlayer with steam until Sterilize at 80-85°C for 15 minutes, cool the interlayer with cooling water to about 50°C inside the tank, start the interlayer water and heater cycle, and heat the heater to 50°C to keep warm;

[0033](2) Start the pH detection terminal, add dilute hydrochloric acid to the bionic enzymolysis tank with a peristaltic pump to adjust the pH value to 1.8, add 3% pepsin in the amount of zein, keep the enzymolysis for 3 hours, monitor the pH value at any time, supplement dilute hydrochloric acid to maintain The pH value is in the range of 1.8-2.2;

[0034] (3) Add 40% sodium hydroxide to the bionic enzymolysis tank to adjust the pH value to ...

Embodiment 3

[0037] Example 3 corn peptide powder:

[0038] Get the corn peptide powder obtained by the industrial production method described in Example 1, after testing: its total protein content is 92%, the trichloroacetic acid soluble small molecule peptide content is 86%, the free amino acid content is 3.7%, and the moisture content is 5.3%, and the relative molecular weight distribution is:

[0039]

[0040]

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PUM

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Abstract

The invention discloses an industrial production method of preparing corn peptide powder by means of bionic enzymatic hydrolysis from corn proteins. The method is characterized by comprising the step of performing bionic enzymatic hydrolysis, high-speed centrifugation, ultrafiltration, electrodialysis, concentration and drying to obtain the corn peptide powder by taking the corn proteins as a raw material. The total protein content of the corn peptide powder is 90% above, the content of small molecule peptide which can be dissolved by trichloroacetic acid is 85% above, and the corn peptide powder has the advantages of meeting the selecting mechanism of human absorption, being free of toxic and side effects and low in cost and the like, and can be applied to food, health food and drugs.

Description

technical field [0001] The invention relates to a preparation method of corn peptide powder, in particular to an industrial production method for preparing corn peptide powder from corn protein through biomimetic enzymolysis. Background technique [0002] The main by-product of corn production and processing is corn gluten meal, its protein content is 67% to 71%, and the remaining ingredients are 12% to 15% of starch, 3% to 7% of fat, 1% to 2% of cellulose and 1% ~2% ash etc. The main components in zein are zein (68%), glutenin (28%) and globulin (1.2%). Due to the lack of essential amino acids such as lysine and tryptophan in corn gluten meal, and its special taste and color, and poor water solubility, corn gluten meal is mainly used for roughage production or directly discharged, resulting in a huge waste of resources. waste. [0003] Corn peptide is a peptide molecule with small molecular weight and certain activity obtained after hydrolyzing corn gluten powder with co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/14A23L33/18A61K38/01A61P9/12A61P39/06A61P39/00A61P35/00A61P39/02A61P3/06A61P1/16
CPCA23V2002/00A61K38/011C07K1/14C07K1/34C12P21/06A23V2200/30A23V2200/32A23V2200/326A23V2200/3262A23V2200/334A23V2250/55
Inventor 刘国飞刘玉军
Owner ANHUI BIOLOGICAL PEPTIDE IND RES INST
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