Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Production method for preparing silkworm chrysalis small peptides through bionic enzymatic hydrolysis

A technology of biomimetic enzymolysis and silkworm chrysalis, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides and other directions, can solve the problems of large molecular weight of silkworm chrysalis protein and cannot be directly absorbed by the human body, so as to enhance the curative effect and reduce the incidence of adverse reactions. , the effect of low cost

Inactive Publication Date: 2017-08-29
ANHUI BIOLOGICAL PEPTIDE IND RES INST
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular weight of silkworm chrysalis protein is relatively large, and the human body cannot directly absorb it. It needs to be decomposed into small molecular peptides in the body for the second time to achieve the corresponding biological activity.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Production method for preparing silkworm chrysalis small peptides through bionic enzymatic hydrolysis
  • Production method for preparing silkworm chrysalis small peptides through bionic enzymatic hydrolysis
  • Production method for preparing silkworm chrysalis small peptides through bionic enzymatic hydrolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 The present embodiment comprises the following steps:

[0038] (1) Add 20 liters of purified water to the bionic enzymolysis tank, start the stirring paddle, set the rotating speed at 60 rpm, take 2 kg of silkworm chrysalis protein powder (silkworm chrysalis protein isolate, protein content is more than 90%, moisture is less than 10%), slowly Add, continue to stir and swell for 30 minutes after the addition is complete, heat the interlayer to 80-85°C for 15 minutes to sterilize, pass cooling water to the interlayer to cool down to about 55°C in the tank, start the interlayer water and heater circulation, and the heater heats up Keep warm at 55°C;

[0039] (2) Start the pH detection terminal, add dilute hydrochloric acid to the bionic enzymolysis tank to adjust the pH value to 2.0 with a peristaltic pump, add 1% pepsin (enzyme activity is 3000U / g) of silkworm chrysalis protein, and keep warm for 1 hour for enzymolysis. Monitor the pH value and add dilute hy...

Embodiment 2

[0043] Embodiment 2 The present embodiment comprises the following steps:

[0044] (1) Add 40 liters of purified water to the bionic enzymolysis tank, start the stirring paddle, set the speed at 30 rpm, take 2 kg of silkworm chrysalis protein powder, add it slowly, continue to stir and swell for 30 minutes after the addition, and heat the interlayer with steam until Sterilize at 80-85°C for 15 minutes, cool the interlayer with cooling water to about 50°C inside the tank, start the interlayer water and heater cycle, and heat the heater to 50°C to keep warm;

[0045] (2) Start the pH detection terminal, add dilute hydrochloric acid to the bionic enzymolysis tank to adjust the pH value to 1.8 with a peristaltic pump, add 3% pepsin of silkworm chrysalis protein, keep the enzymolysis for 3 hours, monitor the pH value at any time, supplement dilute hydrochloric acid to maintain The pH value is in the range of 1.8-2.2;

[0046] (3) Add 40% sodium hydroxide to the bionic enzymolysis ...

Embodiment 3

[0049] Embodiment 3 silkworm chrysalis small peptide:

[0050] The silkworm chrysalis small peptide obtained by the industrial production method described in Example 1 was tested: its total protein content was 91%, the trichloroacetic acid soluble small molecule peptide content was 92%, the free amino acid content was 2.1%, and the moisture content is 4.3%, and the relative molecular weight distribution is:

[0051] Relative Molecular Weight Range

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a production method for preparing silkworm chrysalis small peptides through bionic enzymatic hydrolysis. The method is characterized in that silkworm chrysalis protein is used as a base material and subjected to bionic enzymatic hydrolysis, high-speed centrifuging, ultra-filtering, electrodialysis, concentrating and drying to obtain the silkworm chrysalis small peptides. The total protein content of the silkworm chrysalis small peptides is 90% or more; the content of small molecule peptides which can be dissolved with trichloroacetic acid is 85% or more; the silkworm chrysalis small peptides have the advantages of according with a human body absorption selection mechanism, being free of toxic and side effects and low in cost, and the like; the silkworm chrysalis small peptides are applicable to foods, healthcare foods and drugs.

Description

technical field [0001] The invention relates to a preparation method of silkworm chrysalis small peptide, in particular to an industrial production method for preparing silkworm chrysalis small peptide from silkworm chrysalis protein by biomimetic enzymatic hydrolysis. Background technique [0002] Silkworm chrysalis has been a medicine and food for nourishing and strengthening the body, warming the spleen and kidney, and building muscles since ancient times. Silkworm chrysalis has extremely rich nutritional value and medicinal value. It is rich in protein, essential amino acids, vitamins and trace elements. It is an ideal animal food for human beings. Silkworm chrysalis protein is a powdery solid obtained by pulverizing silkworm chrysalis after dehydration and degreasing. The protein content of silkworm chrysalis is more than 50%, which is much higher than that of general food, and the essential amino acids in protein are complete. Silkworm chrysalis protein is composed of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A23L33/18A61K38/01A61P3/04A61P39/00
CPCA23L33/18A23V2002/00A61K38/012C07K1/34C12P21/06A23V2250/55
Inventor 刘国飞王冬韩宇嘉
Owner ANHUI BIOLOGICAL PEPTIDE IND RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com