The invention belongs to the technical field of drugs, and particularly relates to low-concentration tropane drug eye drops. The low-concentration tropane drug eye drops are prepared from 0.0005-0.5%of tropane drug, 0-10% of solute which achieves inclusion and is capable of reducing irritation, 0.01-5% of solute which increases the viscosity of drug liquid, an acidifying or alkalizing agent, an osmotic regulation agent, 0.01-0.05% of bacteriostatic agent and the balance water for injection, wherein the use amount of the acidifying or alkalizing agent enables the pH to be adjusted to be 4.0-6.5, and the use amount of the osmotic regulation agent enables the osmotic pressure to be regulated to 280-330 mOsm/L. The preparation method comprises the steps of S1, the solute which achieves inclusion and is capable of reducing irritation is weighed in proportion and solved into water, the tropane drug is added, and ultrasonic mixing is conducted for 30 minutes in an ultrasonic shake cleaning machine, so that the tropane drug is included by the solute which achieves inclusion and is capable of reducing irritation; S2, ethylparaben, sodium chloride and sodium hyaluronate are weighed in proportion and solved into the water for injection at 40-75 DEG C; S3, the solutions obtained in S1 and S2 are mixed; S4, filtration, sterilization and subpackage are conducted, and the low-concentration tropane drug eye drops are obtained.