Traditional Chinese medicine leech extract product and its application

A leech extract, traditional Chinese medicine technology, applied in the directions of drug combination, hydrolyzed protein components, medical raw materials derived from leeches/helminths, etc., can solve the problems of inappropriateness, lack of purification, purification and separation, low purity, etc., and achieves enhanced activity, improved Bioavailability, the effect of enhancing utilization

Active Publication Date: 2013-08-21
山东禹泽药康产业技术研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The patent with the application number "CN200810226320.3" is the patent applied by the project team for the preparation of leech small peptides by biomimetic enzymatic hydrolysis of leeches. The total small peptide content is not less than 50%, and the purity is low (except for small peptides, Nucleoside components, fatty acids, amino acids, sodium chloride and other components are also more), and contain all small peptides with a relative molecular weight less than 3KDa (including acidic small peptides, neutral small peptides, and basic small peptides), the resulting small peptides have not been refined and purified to isolate the active parts, and it is not suitable to be prepared into injections for direct application

Method used

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  • Traditional Chinese medicine leech extract product and its application
  • Traditional Chinese medicine leech extract product and its application
  • Traditional Chinese medicine leech extract product and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Take 500g of leech, crush it into fine powder, add 10 times the amount of water, boil it, wait until it is cooled to 40°C, and use double enzymes for continuous enzymatic hydrolysis, that is, first add 1.0% pepsin at pH 2.0-2.5 and keep warm for 1 hour , and then add 1.0% trypsin under the condition of pH 8.0-8.5 to insulate for 3 hours, boil, centrifuge at high speed, collect the supernatant, adjust the pH to 6.5-7.0, add 1% activated carbon, stir evenly, filter, The filtrate is ultra-filtered with a hollow fiber membrane with a molecular weight cut-off of 3kD, and the permeate is collected, and then nano-filtered with a ceramic membrane with a molecular weight cut-off of 150D to 300D, and the impermeable liquid is collected and added to a cation exchange resin column. above, eluted with water, collected the eluate, carried out nanofiltration concentration and desalination with a ceramic membrane with a relative molecular mass cut-off of 150D to 300D, and dried to obtai...

Embodiment 2

[0107] Take 500g of leech, crush it into fine powder, add 15 times the amount of water, boil it, wait until it is cooled to 45°C, and perform continuous enzymatic hydrolysis with dual enzymes, that is, first add 1.5% pepsin at pH 1.5-2.0 and keep warm for 2 hours. , and then add 1.5% trypsin under the condition of pH 8.5~9.0 to keep warm for 4 hours, boil, centrifuge, collect the supernatant, add 3% activated carbon, stir evenly, filter, and the filtrate has a molecular weight cut-off of 3kD The hollow fiber membrane is used for ultrafiltration, and the permeate is collected, and then the ceramic membrane with a molecular weight cut-off of 150D-300D is used for nanofiltration, and the non-permeate is collected, and the pH is adjusted to 9.0-10.0, and then added to an anion exchange gel column First, eluted with Tris-hydrochloric acid solution of pH 8.0-9.0, 0.01-0.05M, discarded the eluent, then eluted with 1M sodium chloride solution, collected the eluate, and cut off the rela...

Embodiment 3

[0109] Take 500g of leech, crush it into fine powder, add 12 times the amount of water, boil it, wait until it is cooled to 35°C, and use double enzymes for continuous enzymatic hydrolysis, that is, add 0.5% pepsin at pH 1.5-2.0 and keep warm for 1 hour , and then add 0.5% trypsin under the condition of pH8.5~9.0 to keep warm for 4 hours, boil, let cool, centrifuge, collect the supernatant, adjust the pH6.5~7.0, add 2% activated carbon, stir evenly, filter After filtration, the filtrate is ultrafiltered with a hollow fiber membrane with a molecular weight cutoff of 3kD, and the permeate is collected, and then nanofiltration is performed with a ceramic membrane with a molecular weight cutoff of 150D to 300D, and the impermeable liquid is collected and added to a cation exchange On the resin column, elute with water, collect the eluate, carry out nanofiltration, concentration and desalination with a ceramic membrane with a molecular weight cut-off of 150D~300D, and dry to obtain ...

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Abstract

The invention discloses a traditional Chinese medicine leech extract product and its application and belongs to the field of traditional Chinese medicines. The invention is characterized in that the traditional Chinese medicine leech extract product is prepared by the following steps: carrying out enzymatic hydrolysis on leech by a double-enzyme continuous enzymatic hydrolysis method (leech is firstly subjected to enzymatic hydrolysis under the acidic condition by pepsin, and an enzymolysis suspension is subjected to enzymatic hydrolysis under the alkaline condition by trypsin), carrying out ultrafiltration and nanofiltration, refining by cation exchange gel and the like, and purifying so as to obtain the leech extract product. Purity of the leech extract product reaches more than 90%. Pharmaceutical adjuncts can be added into the leech extract product to prepare a freeze-dried powder injection according to a conventional method. The traditional Chinese medicine leech extract product has a good effect of invigorating blood circulation, is used for treating cardiovascular and cerebrovascular diseases, and especially has a good effect of treating ischemia apoplexy.

Description

technical field [0001] The invention discloses a traditional Chinese medicine leech extract and its application, belonging to the field of traditional Chinese medicine. Background technique [0002] Leech is a kind of medicinal material recorded in the Chinese Pharmacopoeia. It has the functions of dispelling blood, expelling blood stasis, and promoting menstrual flow. Leeches were first recorded in "Shen Nong's Materia Medica", "the main purpose is to expel evil blood, blood stasis, moon closure, accumulation of blood in the abdomen, childlessness, and water passage." Due to the continuous and in-depth research on the treatment of various difficult diseases by promoting blood circulation and removing blood stasis in recent years, the medicinal use of leeches is also favored. [0003] As a traditional Chinese medicine, leeches are widely used clinically in cardiovascular and cerebrovascular diseases. In the way of medicine, the original medicinal materials are usually crush...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61K35/62A61K9/19A61P9/00A61P9/10
Inventor 柴永海杨培民刘国飞邱学伟代龙
Owner 山东禹泽药康产业技术研究院有限公司
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