Yak bone marrow glair polypeptides bone extract and the composition thereof and the preparing method
A technology of yak bone marrow and peptide osteosin, which is applied in food preparation, drug combination, peptide/protein composition, etc., can solve the problems that yak bone cannot be extracted and produced, the production process cannot be simple and effective to use yak bone, and the production process is complicated. The effect of controllable product quality, balanced product composition and simple preparation method
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Embodiment 1
[0052] Example 1 Preparation of Yak Bone Marrow Protein Polypeptide Ostein
[0053] Put 10kg of yak bones that have been cleaned and processed into a cooking tank, add water to the mark, pressurize at 0.2-0.4MPa, heat to 100°C and cook for 4 hours. Carry out oil-water separation, collect the oil into the storage tank for use, put the water layer into the reaction tank, heat and maintain the temperature of the solution in the reaction tank at 80°C for at least half an hour, and then pass tap water through the interlayer to reduce the temperature of the solution in the pot to 50°C. Add the required amount of pancreatin according to the enzyme / yak bone ratio of 1 / 300, and start the enzymolysis reaction under the continuous stirring of the stirrer. The pH of the reaction solution was kept at 8.0, and the enzymolysis reaction ended 6 hours after the addition of trypsin, and the amount of steam introduced was increased to raise the temperature of the enzymolysis solution to 80° C. for...
Embodiment 2
[0054] Example 2 Preparation of yak bone marrow protein polypeptide osteonin of the present invention
[0055] According to the preparation method of Example 1 as shown in Figure 1, the yak bone marrow protein polypeptide osteonin was prepared by screening the cooking time, the amount of hydrolytic enzyme, the hydrolysis time, and the spray drying temperature, and the screening results are shown in Test Examples 1-4.
Embodiment 3
[0056] Embodiment 3 Preparation of osteotin composition of the present invention
[0057] As shown in Fig. 2, the composition of the present invention is prepared by changing the weight ratio of the plant additive and the yak bone marrow protein polypeptide bone protein. The weight ratio is as follows:
[0058] Yak bone marrow protein polypeptide bone protein: white fungus polysaccharide = 700g: 200g; another 500g: 100g; another 700g: 300g.
[0059] Yak bone marrow protein polypeptide bone protein: lentinan = 700g: 200g; another 900g: 300g; another 800g: 100g.
[0060] Yak bone marrow protein polypeptide bone protein: Ganoderma lucidum polysaccharide = 800g: 100g; another 900g: 100g; another 900g: 200g.
[0061] Yak bone marrow protein polypeptide bone protein: aloe polysaccharide = 700g: 200g; another 400g: 100g; another 400g: 300g.
[0062] Yak bone marrow protein polypeptide bone protein: Artemisia persicae oil = 700g: 200g; another 700g: 600g; another 400g: 500g.
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