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50 results about "Protein processing" patented technology

Proteins are produced by stringing amino acids together in the order specified by messenger RNA strands that were transcribed from DNA in the cell nucleus. The process of synthesizing a protein is called translation, and it occurs on ribosomes in the cytoplasm of a cell.

Modified beta-lactoglobulin as well as preparation method and application thereof

PendingCN120923603AFood ingredient as antioxidantFood ingredient as foaming agentHydrolysateGlutaminase
The invention belongs to the technical field of protein processing, and particularly relates to modified beta-lactoglobulin as well as a preparation method and application thereof. The modified beta-lactoglobulin is a composite modified product which is obtained by performing a papain hydrolysis treatment process on beta-lactoglobulin to obtain a hydrolysate and then performing glycosylation modification on the hydrolysate by adopting chitosan oligosaccharide and transglutaminase. The product has significantly improved oxidation resistance, emulsifying properties and foaming activity, and is suitable as a food processing ingredient, such as an antioxidant, an emulsifier or a foaming agent.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

High-emulsification-activity gluten protein based on irradiation modification and preparation method thereof

The invention discloses a high-emulsification-activity gluten protein based on irradiation modification and a preparation method thereof in the field of wheat protein processing, and the method comprises the following steps: dispersing wheat gluten protein in deionized water, stirring to form a uniform suspension, adjusting the pH value, carrying out enzymolysis treatment on the suspension by using alkaline protease, and drying to obtain the high-emulsification-activity gluten protein. Centrifugally drying to obtain primary protein powder; dissolving the protein primary powder in an irradiation solvent to obtain a protein dispersion liquid, and irradiating the protein dispersion liquid by using cobalt-60 gamma rays as an irradiation source in an intermittent manner; and taking the irradiated protein dispersion liquid, and performing centrifugal drying to obtain the high-emulsification active gluten protein. The method comprises the following steps: firstly, carrying out limited hydrolysis on gluten protein by using protease to generate a smaller peptide fragment, so that the irradiation efficiency and the emulsion stability are improved; by adopting an intermittent irradiation treatment mode, protein aggregation caused by heat effect can be reduced, the emulsifying effect is improved, and the stability and adhesive force of an adhesive can be improved when the emulsion is applied to the adhesive.
Owner:河南省科学院同位素研究所有限责任公司 +1

An inhibitor for inhibiting KRAS phase separation to exert an anti-tumor effect and application

PendingCN122251597AExtended Treatment Strategiesinhibit processingDigestive systemRespiratory disorderTumor therapyTherapeutic effect
The application discloses an inhibitor for inhibiting KRAS phase separation to play an anti-tumor role and application, and relates to the fields of tumor molecular biology and targeted therapy.The application aims at solving the problems of limited effect, strong drug resistance and limited application range of KRAS tumor treatment methods, and the inhibitor is a statin.The KRAS protein can form a liquid condensate in cells, and the application discloses the mechanism of the KRAS protein in promoting KRAS protein processing, transportation and downstream signal activation, and further provides a technical scheme for inhibiting tumor growth and enhancing the therapeutic effect of a KRAS inhibitor by inhibiting the formation of KRAS condensates, so as to provide a new target and intervention approach for tumor treatment.
Owner:HARBIN INST OF TECH

Method for improving bioavailability of animal feed protein through chemical enzymolysis

The invention belongs to the field of feed protein processing, and particularly relates to a method for improving the bioavailability of animal feed protein through chemical enzymolysis. The method comprises the following steps: mixing a compound protein raw material with water to form a suspension; sodium tripolyphosphate is added for phosphorylation pretreatment, so that protein is subjected to specific modification; after the pH is adjusted to be acidic, pepsase and acid phosphatase are added at the same time for an enzymolysis reaction; and after the reaction is finished, performing enzyme deactivation and drying to obtain the modified feed protein. According to the method, a specific enzyme cutting site is created on a protein molecule through chemical phosphorylation, then directional hydrolysis is achieved through efficient recognition of pepsin on the site, and meanwhile dynamic regulation and control of the hydrolysis process are achieved by means of the dephosphorylation effect of acid phosphatase. The obtained product has the outstanding advantages of high content of small peptides, good digestive absorbability, low bitterness, strong biological activity and the like, and the bioavailability of feed protein is remarkably improved.
Owner:GUIZHOU UNIV

Collagen peptide composition with function of increasing muscle strength and application of collagen peptide composition

The invention discloses a collagen peptide composition with a function of increasing muscle strength and application of the collagen peptide composition. The collagen peptide composition comprises fish collagen peptide and beef tendon collagen peptide. A mouse senescence sarcopenia model is constructed, then a collagen peptide composition is used for intragastric intervention, and the improvement effect is evaluated through behavioral testing, body component analysis and HE staining. The potential target and regulatory pathway of the collagen peptide composition are explored by combining network pharmacology and molecular docking. Results show that the four-limb grip strength, treadmill exhaustion time and movement distance of a model mouse can be remarkably improved, quadriceps femoris muscle and gastrocnemius muscle indexes are recovered, and muscle fiber atrophy and collagen deposition are relieved. And it is found that TNF, IL1B, AKT1, mTOR and MAPK1 may be core targets of the TNF, IL1B, AKT1, mTOR and MAPK1. The polypeptide plays a role by regulating and controlling biological processes such as MAPK cascade, protein processing and inflammatory reaction, cell apoptosis, a cAMP pathway and a calcium signal pathway.
Owner:HAINAN PURE PEPTIDE TECH CO LTD

Multifunctional liquid nitrogen device for earthworm protein production

The utility model relates to the technical field of earthworm protein processing, in particular to a multifunctional liquid nitrogen device for earthworm protein production, which comprises a processing cabinet, a cabinet door, a blanking hopper, a hopper plug, a liquid inlet, a connecting pipe, a liquid nitrogen spray head and the like, a cabinet door is rotationally connected to one side of the treatment cabinet, a discharging hopper is fixedly connected to the top of the treatment cabinet, a hopper plug used for sealing an opening of the discharging hopper is movably connected to the discharging hopper, a hopper neck of the discharging hopper extends into the treatment cabinet and is provided with a liquid nitrogen spray head, a liquid inlet is formed in the position, close to the discharging hopper, of the treatment cabinet, and the liquid inlet is communicated with the liquid nitrogen spray head through a connecting pipe. According to the quick-freezing and grinding device, through cooperation of the liquid nitrogen spray head, the grinding frame, the grinding rod, the driving mechanism and other parts, tight connection of quick-freezing work and grinding work is achieved, then the wind direction of degradation and denaturation caused by raw material transfer is avoided, and the production efficiency and the product quality are effectively improved.
Owner:TIANJIN MINSHENG BIOLOGICAL ENG CO LTD

Corn enzymolysis protein with high protein content and high micromolecule active substances and preparation method of corn enzymolysis protein

The invention belongs to the technical field of corn protein processing, and particularly relates to corn enzymolysis protein with high protein content and high micromolecule active substances and a preparation method. The method comprises the following steps: S1, weighing corn protein powder, adding water into the corn protein powder, stirring, adding alkali to adjust the pH value, and then adding medium-temperature amylase for enzymolysis; s2, separating the materials to obtain filter residues; s3, adding water into the materials, stirring, and adding acidic cellulase for enzymolysis; and S4, raising the temperature of the material, adjusting the pH value with alkali, and adding alkaline protease for enzymolysis. The corn enzymolysis protein prepared by the method can be used as a high-quality functional protein to be applied to aquatic feed, so that the intestinal and liver health of aquatic animals is remarkably improved, the total dosage of protein raw materials in the aquatic feed is reduced, and the dosage of imported protein resources (such as fish meal) is reduced; pigmentation can be promoted, and the commodity performance of aquatic animals can be improved; therefore, the economic benefits of aquatic feed enterprises and farmers are improved.
Owner:CHENGDU MYTECH BIOTECH

Hydrolyzed silk protein preparation method and integrated reaction device

The invention relates to the technical field of silk protein processing equipment, in particular to a hydrolyzed silk protein preparation method and an integrated reaction device.The integrated reaction device comprises a workbench, the workbench is provided with a cleaning unit, a drying unit, a smashing and separating unit and a hydrolyzed silk protein preparation integrated unit, and the cleaning unit comprises a cleaning tank; an ultrasonic generator is arranged on the inner wall of the cleaning tank, a water drainage cavity is formed in the bottom of the cleaning tank, a water suction pump is arranged in the water drainage cavity, the units for cleaning, drying, smashing and separating and hydrolyzed silk protein preparing are integrated on the workbench, and the occupied area of the equipment is reduced; automatic material transfer is achieved among the units through auxiliary assemblies, the repeated transfer link of hydrolyzed silk among different processing containers is omitted, the manual operation intensity is reduced, meanwhile, the whole process of hydrolyzed silk protein preparation is integrated, the production process is safe, simple, convenient and economical, and industrial large-scale production is easy to achieve.
Owner:CHONGHAI HAIFAN BIOCHEMICAL TECH CO LTD

Method for improving emulsibility and thermal stability of whey protein isolate

The invention discloses a method for improving emulsibility and thermal stability of whey protein isolate, and relates to the technical field of protein processing. The preparation method comprises the following specific steps: respectively dissolving whey protein isolate, sodium alginate and steviol glycoside in water, and fully hydrating to obtain a whey protein isolate solution, a sodium alginate solution and a steviol glycoside solution; adjusting the pH values of the three solutions, and mixing according to a certain sequence and proportion to obtain a mixed solution; the obtained mixed solution has good emulsibility and thermal stability, and compared with single WPI, the emulsion stability is increased by 25.2 times. And a practical method is provided for improving the environmental sensitivity and emulsibility of the whey protein isolate.
Owner:JIANGSU UNIV

A process for the processing of whey proteins

The application belongs to the technical field of protein processing, and particularly relates to a processing technology of whey protein, which comprises the following steps: subjecting cow milk to homogenization treatment; adding a foaming substance into the homogenized liquid, wherein the foaming substance is bicarbonate and citric acid, and the addition amount of the foaming substance is 3-7% of the mass of fresh cow milk; collecting the supernatant of the cow milk under the condition of high-speed centrifugation at room temperature; and separating and removing casein in the supernatant of the cow milk to obtain whey protein liquid of the cow milk. In the application, the addition of the foaming substance can change the structure of casein colloidal particles, so that casein is separated in large quantities, the purity of the whey protein is improved, a complicated purification step is not needed, the purification cost of the whey protein is reduced, and resources are saved.
Owner:JINZHOU MEDICAL UNIV

Solid-liquid separation device for earthworm protein processing

The utility model discloses a solid-liquid separation device for earthworm protein processing, and relates to the technical field of earthworm protein processing, the solid-liquid separation device comprises a collection cylinder, a liquid guide pipe is rotatably arranged in the middle of the top end of the collection cylinder, the liquid guide pipe is driven by a driving mechanism to rotate, a liquid guide cylinder is arranged at the top end of the liquid guide pipe, a separation cylinder is arranged in the liquid guide cylinder, and a liquid outlet is formed in the separation cylinder. Through holes are formed in the bottom end and the bottom of the side face of the separation barrel, and a material pressing mechanism is arranged at the top end of the separation barrel; the earthworm protein solid-liquid separation device has the advantages that the driving mechanism can drive the separation barrel to rotate quickly, so that solid-liquid separation is performed on earthworm protein, solids are reserved in the separation barrel, the separated solids can be compressed by the aid of the pressing mechanism, liquid attached to the surfaces of the solids can be discharged, and the earthworm protein solid-liquid separation device is simple in structure and convenient to use. And under the action of a liquid guide barrel and a liquid guide pipe, the liquid is discharged into a collecting barrel to be collected.
Owner:济宁华能制药厂有限公司

Processing and extracting device for protein

The invention relates to the technical field of plant protein processing and extraction, in particular to a protein processing and extracting device which comprises an equipment cabin, the interior of the equipment cabin is divided into an upper cabin body and a lower cabin body through a partition plate, and a centrifugal assembly and a separation assembly are arranged in the equipment cabin; the centrifugal assembly comprises a rotating body arranged in the upper cabin body, a liquid cabin is formed in the middle of the rotating body, an inner gear is mounted in the middle of the rotating body, a mounting part is mounted in the middle of the partition plate, a second motor is mounted at the top end of the mounting part, and a second gear is mounted on an output shaft of the second motor; according to the scheme, the centrifugal assembly containing the staggered sealing strips and the locking assembly and the separation assembly with the one-way liquid passing hole are integrated, and the operation opening and the flushable structure on the periphery of the equipment cabin are matched, so that continuous treatment of soybean protein from alkaline soluble protein, centrifugal impurity removal to acid precipitation protein extraction is achieved; meanwhile, the problems of complicated split operation, easy leakage, impurity reflux and inconvenience in maintenance of the traditional device are solved, and the extraction efficiency and the target protein purity are guaranteed.
Owner:BINZHOU POLYTECHNIC

A protein processing flocculation device

The utility model discloses a protein processing flocculation device, including flocculation jar body, the top of flocculation jar body assembly has the jar top cover, and the inside bottom of flocculation jar body assembly is assembled with the water distribution board, the top center of jar top cover is equipped with the driving hole, and is provided with the pivot in the driving hole, the top center of water distribution board assembly has the fixed base, and the bottom of pivot installs in fixed base, and the top of water distribution board is evenly equipped with the through -hole, the outside of pivot is installed with the axle cylinder close to fixed base, both sides of axle cylinder all are installed with the scraper, and the bottom of scraper is installed with the cleaning assembly from left to right equidistance, the setting of this protein processing flocculation device, the structure design is reasonable, through setting cleaning assembly, because the upside of sliding plate is inclined and sets, after the rotation of stirring blade and the resistance of sliding plate, force sliding plate to move downward, make the cleaning rod export the blockage in the through -hole, thereby reach the prevention flocculating material and cause the blockage to the through -hole, thereby influence processing efficiency.
Owner:NINGAN CEREALS OILS & STARCH MACHINERY MFG

Soy protein emulsion gel product and preparation method thereof

The present invention provides a soy protein emulsion gel product and a preparation method thereof, belonging to the technical field of plant protein processing. Using soy protein as raw material, the invention first enzymatically hydrolyzes and heat-treats soy protein to obtain soy protein with different 7S / 11S ratios and soluble aggregate contents. Oil is then added and emulsified to prepare a soy protein emulsion. On this basis, glucono-δ-lactone is used to prepare an acid-induced emulsion gel. This method achieves the preparation of a soy protein emulsion gel with excellent textural properties and microstructure, broadening the application of soy protein in food and related fields.
Owner:JIANGNAN UNIV

A method for efficiently extracting soybean peptides

The application belongs to the field of protein processing, and particularly relates to a method for efficiently extracting soybean peptides. The method comprises the following steps: preparing soybean protein into a suspension, and performing ultrasonic pretreatment, then adding a chlorogenic acid grafted UiO-66 biomimetic catalyst, performing catalytic hydrolysis reaction under weak alkaline conditions, and obtaining soybean peptides after separation and purification. The core of the application is that the catalyst innovatively combines the protein recognition and enrichment function of chlorogenic acid with the Zr 6+ hydrolysis active center of UiO-66, significantly improving the efficiency of catalytic hydrolysis of proteins. The yield of soybean peptides obtained by the method is as high as 88.5%, and the catalyst can be recycled for more than 5 times with an activity retention rate of more than 87.5%, greatly reducing the cost. More outstandingly, the obtained soybean peptides have excellent antioxidant activity, and the DPPH free radical scavenging capacity is improved by about 52% compared with the traditional enzyme product, realizing the overall improvement from the production process to the quality of the terminal product.
Owner:YANTAI SAICHUN BIOTECHNOLOGY CO LTD

A fully automatic recovery device based on wheat protein

The present invention discloses a fully automatic recovery device based on wheat protein, which includes a recovery pool, a washing water discharge pipeline, a granular wheat protein filtration component, a main wheat protein collection pipe, a fluffy wheat protein filtration component, a wheat protein collection branch pipe, and a liquid discharge pipeline; a washing water discharge pipeline is provided at the upper end of the recovery pool, a granular wheat protein filtration component is provided below the washing water discharge pipeline, at least one group of fluffy wheat protein filtration components is provided in the recovery pool, and a liquid discharge pipeline for discharging the filtered washing water is provided at the end in the recovery pool. By arranging a granular wheat protein filtration component and a fluffy wheat protein filtration component in the recovery pool, the present invention effectively separates the broken wheat protein in the water body, realizes the recycling of granular and fluffy wheat protein, improves the utilization rate of production raw materials, and reduces the production cost during wheat protein processing.
Owner:JIANGSUCHINA PALARICH

A subcritical continuous protein hydrolysis system

This invention discloses a subcritical continuous hydrolysis system for proteins, primarily relating to the field of protein processing equipment. It includes a raw material storage tank, a high-temperature pressure reactor, and a product storage tank, sequentially connected by pipelines. The high-temperature pressure reactor is externally equipped with a temperature control jacket, and internally contains a stirring paddle. A discharge valve with pressure monitoring is installed at the top of the reactor; the discharge valve opens when the pressure exceeds a threshold. The discharge valve is connected to a discharge pipeline, which includes one or more heat exchangers. One or more preheaters are located between the raw material storage tank and the high-temperature pressure reactor, and a closed circulation pipeline connects at least one preheater and at least one heat exchanger. The advantages of this invention are: achieving efficient and continuous high-temperature, high-pressure hydrolysis of proteins, improving production efficiency and product uniformity, and significantly reducing production energy consumption.
Owner:SHANDONG MUKANG FENGWO BIOTECHNOLOGY CO LTD

Modified casein-polyphenol compound as well as preparation method and application thereof

The invention provides a modified casein-polyphenol compound as well as a preparation method and application thereof, and belongs to the technical field of protein processing. The invention provides the application of the small red garlic polyphenol in casein modification for the first time, and further provides a preparation method of a modified casein-small red garlic polyphenol compound, and the preparation method comprises the following steps: preparing a casein culture solution, inoculating casein casei for fermentation, and sterilizing after fermentation to obtain a fermentation solution; mixing the small red garlic polyphenol with the fermentation liquor for reaction, and drying a reaction product to obtain the modified casein-small red garlic polyphenol covalent complex. The stability, the oxidation resistance, the solubility, the emulsifying capacity, the emulsifying stability, the foamability and the foaming stability of the casein can be obviously improved, and the method has a wide application prospect in the field of protein processing.
Owner:SOUTHWEST UNIVERSITY FOR NATIONALITIES +2

Purification method and application of lipoprotein lipase

The invention belongs to the technical field of protein processing, and particularly relates to a purification method and application of lipoprotein lipase. The purification method of the lipoprotein lipase comprises the following steps: inserting a His tag into a coding gene of a lipase specific folding enzyme, preparing the lipase specific folding enzyme with the His tag, carrying out affinity purification, mixing the lipase specific folding enzyme with lipoprotein lipase, incubating, purifying again, and recovering the lipase specific folding enzyme to obtain the lipoprotein lipase. According to the purification method provided by the invention, the harm of a high-salt and high-imidazole environment to the protein in a traditional purification method can be avoided, the problem of protein aggregation in the purification process is solved, mild elution is realized, the inhibition effect of free lifO protein can also be avoided, and a new way is provided for simply, conveniently, efficiently and rapidly purifying the protein.
Owner:JIAPU TIANCHENG (SHANGHAI) BIOTECHNOLOGY CO LTD

Drainage device for protein purification equipment

The utility model relates to the technical field of biological protein processing and production, in particular to a drainage device for protein purification equipment, which comprises a connecting base, a connecting seat, a connecting sleeve, a connecting vertical pipe, a supporting seat, a dense separation net, a molecular sieve filling part, a top net cover, a sampling pipe and a drainage pipe, an opening is formed in the middle of the upper end of the connecting base, the connecting sleeve is fixedly connected to the surface of the edge position of the opening of the connecting base, internal threads are formed in the inner surface of the connecting sleeve, external threads are arranged at the lower end of the connecting vertical pipe, and the lower end of the connecting vertical pipe is in threaded connection with the inner surface of the connecting sleeve. The supporting seat is fixedly connected to the upper surface of the middle position of the connecting base, the dense separation net is connected to the upper end of the supporting seat in a matched mode, and the device has the effects of being high in operation convenience degree, high in filtering efficiency, capable of improving the drainage speed, convenient to clean and not prone to waste.
Owner:LUOYANG JIBAI BIOTECHNOLOGY CO LTD

Method for improving emulsifying property of soybean protein isolate by combined treatment of enzymolysis and oxidized carboxymethyl cellulose

The invention relates to a method for improving emulsifying property of soybean protein isolate by combined treatment of enzymolysis and oxidized carboxymethyl cellulose, which belongs to the technical field of protein processing and comprises the following steps: (1) enzymolysis treatment of soybean protein isolate (SPI); (2) preparing oxidized carboxymethyl cellulose (OCMC); and (3) respectively stirring and mixing the SPH and the SPI with the OCMC, and storing at 4 DEG C overnight to obtain SPH-OCMC and SPI-OCMC compounds. According to the modification method disclosed by the invention, the soybean protein isolate with good emulsifying property can be obtained, the emulsifying activity is improved by 63.64%, the emulsifying stability is improved by 1.82 times, the application value of the soybean protein is further expanded, and the modification method has very important significance in expanding the application range and prospect of the soybean protein in the fields of food industry and the like and has a wide application prospect. Meanwhile, the method has important strategic significance for promoting the upgrading of a soybean agricultural product deep processing technology.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Method for synergistically improving solubility of quinoa protein based on enzymolysis of brown rice starch and pH shift

The invention discloses a method for synergistically improving the solubility of chenopodium quinoa protein based on composite enzymolysis of brown rice starch and pH shift, and belongs to the technical field of chenopodium quinoa protein processing. The method comprises the steps of quinoa protein extraction, brown rice starch extraction, brown rice starch composite enzymolysis, pH deviation treatment, 0.1 mol / L HCL callback, freeze drying and the like. Various amylolytic enzymes such as pullulanase and alpha-amylase and a compounding scheme are screened, and process parameters are optimized, so that the solubility of the modified quinoa protein reaches 88.94%-92.15%, the absolute value of the Zeta potential is 20-30 mV, and functional characteristic differentiation is adaptive to different food scenes. The composite enzymolysis and pH shift synergistic effect mechanism is clear, the process is stable and repeatable, the problems of limited modification effect and complex operation in the prior art are solved, and the method has important industrial application value.
Owner:NINGBO ZHENGU FOOD TECHNOLOGY CO LTD

A processing technology of desolventized and dephenolated cottonseed protein

PendingCN122642491AProtein processingDegreasing
The application discloses a kind of cottonseed protein processing technology of desolventizing and dephenolization, and relates to the technical field of cottonseed processing.The application comprises the following steps: S1, cottonseed pretreatment;S2, cottonseed cotton oil removal treatment;S3, cottonseed dephenolization treatment;S4, wet meal is treated, and a large amount of solvent in wet meal is separated by the cooperation of evaporation tower and drying tower;S5, wet meal is treated, and wet meal is desolventized and dried by gradually increasing temperature in stages.The application provides a kind of processing technology for cottonseed protein, which is dephosphorized and dephenolized, and residual solvent is removed, so that cottonseed protein can be obtained as edible material, and the utilization rate of cottonseed is improved.
Owner:XINJIANG XINSAI BIOLOGICAL PROTEIN TECH CO LTD +1

Method for improving performance of concentrated milk protein and concentrated milk protein

The invention belongs to the technical field of protein processing, and particularly relates to a method for improving the performance of concentrated milk protein and the concentrated milk protein. And immersing an ultrasonic probe into the concentrated milk protein solution, and carrying out probe ultrasonic treatment for 5-30 minutes under the power of 100-500W. By adopting the method provided by the invention, after the concentrated milk protein is subjected to probe ultrasonic treatment, the solubility of the concentrated milk protein is increased from 39.63% to 94.44%, which indicates that the probe ultrasonic treatment can destroy protein aggregates through the cavitation effect and improve the solubility and dispersion stability of the protein aggregates, thereby providing a theoretical basis for efficient application of the concentrated milk protein in the food industry.
Owner:SICHUAN AGRI UNIV

Protein processing method, device, computer device, and storage medium

Embodiments of the present application disclose a protein processing method and device, computer equipment and a storage medium, belonging to the technical field of computer. The method comprises: displaying an input first protein sequence and a mutation statement, the mutation statement indicating mutation of the first protein sequence, the mutation statement comprising at least one mutation instruction, the mutation instruction comprising a mutation position and a mutation mode; in response to a processing request for the first protein sequence and the mutation statement, for each mutation instruction in the mutation statement, mutating the mutation position in the first protein sequence according to the mutation mode in the mutation instruction to obtain a second protein sequence; and displaying the second protein sequence. The protein sequence is mutated based on the mutation statement, the process of protein mutation is simulated, the mutated protein is obtained, and thus the process of mutating the protein is simplified, the efficiency of mutating the protein is improved, and the efficiency of protein processing is further improved.
Owner:TENCENT TECHNOLOGY (SHENZHEN) CO LTD

Multistage efficient earthworm protein filtering device

The utility model discloses a multistage efficient filtering device for earthworm protein, which relates to the technical field of earthworm protein processing equipment and comprises a feed hopper, a support frame is mounted in the feed hopper, a protective sleeve is mounted at the top of the support frame, and a power motor is mounted in the protective sleeve. According to the earthworm protein filtering device, the limiting rod, the limiting spring, the secondary filtering sleeve, the corrugated sealing sleeve, the fixing shaft, the fixing sleeve and the primary filtering sleeve are arranged, the corrugated sealing sleeve is in threaded connection with the secondary filtering sleeve and the primary filtering sleeve through the fixing shaft and the fixing sleeve respectively for sealing, the situation that earthworm protein is scattered in the filtering process and waste is caused is avoided, and meanwhile the earthworm protein filtering device is convenient to use. Through the arrangement of the second-stage filter sleeve and the first-stage filter sleeve, the earthworm protein powder can be subjected to multi-stage fine filtration, the purity and the quality of the earthworm protein are improved, the second-stage filter sleeve can be pulled to do vertical reciprocating motion in the filtration process, the earthworm protein powder on the surface of the second-stage filter sleeve is vibrated, and the filtration efficiency is improved.
Owner:DILONGLI BIOENGINEERING (TIANJIN) CO LTD

Recombinant bovine leukemia virus infectious clone plasmid as well as preparation method and application thereof

The invention discloses a recombinant bovine leukemia virus infectious clone plasmid, a preparation method and application, the recombinant plasmid is based on the bovine leukemia virus infectious clone plasmid, and an IRES element and a target gene are inserted into a non-coding region between an Env gene and a Tax gene of a bovine leukemia virus fragment. When the recombinant bovine leukemia virus infectious clone plasmid is used for transfecting cells and then culturing, the recombinant bovine leukemia virus capable of stably expressing a target gene can be successfully rescued and obtained, the protein processing modification after the infectious clone plasmid translation is not influenced, and the assembly of filial generation virus particles is not influenced; the expressed progeny virus can efficiently express a target gene, can be stably inherited in continuous passage, and has a relatively high application value.
Owner:YANGZHOU UNIV

Silkworm chrysalis peptide with anti-aging effect as well as preparation method and pharmaceutical preparation of silkworm chrysalis peptide

The invention relates to the technical field of protein treatment, in particular to silkworm chrysalis peptide with an anti-aging effect and a preparation method and a medicinal preparation thereof, and the preparation method comprises the following steps: mixing silkworm chrysalis protein and water, performing microwave heating treatment, adding a first strain, performing first reaction, inactivating, mixing an obtained first reactant with a second strain, and performing second reaction to obtain a second reactant; and carrying out inactivation after a second reaction, mixing the obtained second reactant with protease, and carrying out inactivation after a third reaction so as to obtain the silkworm chrysalis peptide. Silkworm chrysalis protein is sequentially subjected to microwave heating treatment, specific fermentation and enzymolysis, the silkworm chrysalis peptide with good anti-aging activity can be obtained, and the obtained silkworm chrysalis peptide is white to faint yellow, smells slightly fishy and comprises polypeptide composed of various 3-10 amino acids; the polypeptides can play an anti-aging role by reducing the oxidative stress level, and compared with many chemically synthesized anti-aging drugs, the polypeptides have higher safety and fewer side effects, and can stably play an anti-aging role for a long time.
Owner:SHENZHEN UNIV

Synergistic modification method of soybean protein-casein compound and product

The invention discloses a synergistic modification method of a soybean protein-casein compound and a product, and belongs to the technical field of food protein processing and modification.The method comprises the steps that SPI and CS are mixed according to the mass ratio selected from (9: 1) to (1: 9) and dissolved in water, dispersion liquid with the concentration being 4%-8% is prepared, the pH is adjusted to 10-12.0 after hydration, and stirring is conducted; carrying out ultrasonic treatment on the protein liquid under the condition of keeping the alkaline pH unchanged; finally, the pH is adjusted back to be neutral, centrifugation is conducted, and supernate is taken to obtain the modified compound. According to the method, an ultrasonic cavitation effect acts on protein molecules which are expanded in an alkaline manner to generate a synergistic effect, and protein conformation is induced to generate flexible rearrangement, so that the solubility, emulsification, foaming and other interface functions of the compound are synergistically and remarkably improved. The invention provides a green and efficient way for constructing a high-performance plant-animal double-protein ingredient.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY