34 results about "Protein processing" patented technology

The invention belongs to the technical field of protein processing, and particularly relates to modified beta-lactoglobulin as well as a preparation method and application thereof. The modified beta-lactoglobulin is a composite modified product which is obtained by performing a papain hydrolysis treatment process on beta-lactoglobulin to obtain a hydrolysate and then performing glycosylation modification on the hydrolysate by adopting chitosan oligosaccharide and transglutaminase. The product has significantly improved oxidation resistance, emulsifying properties and foaming activity, and is suitable as a food processing ingredient, such as an antioxidant, an emulsifier or a foaming agent.

The application discloses an inhibitor for inhibiting KRAS phase separation to play an anti-tumor role and application, and relates to the fields of tumor molecular biology and targeted therapy.The application aims at solving the problems of limited effect, strong drug resistance and limited application range of KRAS tumor treatment methods, and the inhibitor is a statin.The KRAS protein can form a liquid condensate in cells, and the application discloses the mechanism of the KRAS protein in promoting KRAS protein processing, transportation and downstream signal activation, and further provides a technical scheme for inhibiting tumor growth and enhancing the therapeutic effect of a KRAS inhibitor by inhibiting the formation of KRAS condensates, so as to provide a new target and intervention approach for tumor treatment.

The invention belongs to the field of feed protein processing, and particularly relates to a method for improving the bioavailability of animal feed protein through chemical enzymolysis. The method comprises the following steps: mixing a compound protein raw material with water to form a suspension; sodium tripolyphosphate is added for phosphorylation pretreatment, so that protein is subjected to specific modification; after the pH is adjusted to be acidic, pepsase and acid phosphatase are added at the same time for an enzymolysis reaction; and after the reaction is finished, performing enzyme deactivation and drying to obtain the modified feed protein. According to the method, a specific enzyme cutting site is created on a protein molecule through chemical phosphorylation, then directional hydrolysis is achieved through efficient recognition of pepsin on the site, and meanwhile dynamic regulation and control of the hydrolysis process are achieved by means of the dephosphorylation effect of acid phosphatase. The obtained product has the outstanding advantages of high content of small peptides, good digestive absorbability, low bitterness, strong biological activity and the like, and the bioavailability of feed protein is remarkably improved.

The invention discloses a collagen peptide composition with a function of increasing muscle strength and application of the collagen peptide composition. The collagen peptide composition comprises fish collagen peptide and beef tendon collagen peptide. A mouse senescence sarcopenia model is constructed, then a collagen peptide composition is used for intragastric intervention, and the improvement effect is evaluated through behavioral testing, body component analysis and HE staining. The potential target and regulatory pathway of the collagen peptide composition are explored by combining network pharmacology and molecular docking. Results show that the four-limb grip strength, treadmill exhaustion time and movement distance of a model mouse can be remarkably improved, quadriceps femoris muscle and gastrocnemius muscle indexes are recovered, and muscle fiber atrophy and collagen deposition are relieved. And it is found that TNF, IL1B, AKT1, mTOR and MAPK1 may be core targets of the TNF, IL1B, AKT1, mTOR and MAPK1. The polypeptide plays a role by regulating and controlling biological processes such as MAPK cascade, protein processing and inflammatory reaction, cell apoptosis, a cAMP pathway and a calcium signal pathway.

The utility model relates to the technical field of earthworm protein processing, in particular to a multifunctional liquid nitrogen device for earthworm protein production, which comprises a processing cabinet, a cabinet door, a blanking hopper, a hopper plug, a liquid inlet, a connecting pipe, a liquid nitrogen spray head and the like, a cabinet door is rotationally connected to one side of the treatment cabinet, a discharging hopper is fixedly connected to the top of the treatment cabinet, a hopper plug used for sealing an opening of the discharging hopper is movably connected to the discharging hopper, a hopper neck of the discharging hopper extends into the treatment cabinet and is provided with a liquid nitrogen spray head, a liquid inlet is formed in the position, close to the discharging hopper, of the treatment cabinet, and the liquid inlet is communicated with the liquid nitrogen spray head through a connecting pipe. According to the quick-freezing and grinding device, through cooperation of the liquid nitrogen spray head, the grinding frame, the grinding rod, the driving mechanism and other parts, tight connection of quick-freezing work and grinding work is achieved, then the wind direction of degradation and denaturation caused by raw material transfer is avoided, and the production efficiency and the product quality are effectively improved.

The invention relates to the technical field of silk protein processing equipment, in particular to a hydrolyzed silk protein preparation method and an integrated reaction device.The integrated reaction device comprises a workbench, the workbench is provided with a cleaning unit, a drying unit, a smashing and separating unit and a hydrolyzed silk protein preparation integrated unit, and the cleaning unit comprises a cleaning tank; an ultrasonic generator is arranged on the inner wall of the cleaning tank, a water drainage cavity is formed in the bottom of the cleaning tank, a water suction pump is arranged in the water drainage cavity, the units for cleaning, drying, smashing and separating and hydrolyzed silk protein preparing are integrated on the workbench, and the occupied area of the equipment is reduced; automatic material transfer is achieved among the units through auxiliary assemblies, the repeated transfer link of hydrolyzed silk among different processing containers is omitted, the manual operation intensity is reduced, meanwhile, the whole process of hydrolyzed silk protein preparation is integrated, the production process is safe, simple, convenient and economical, and industrial large-scale production is easy to achieve.

The invention discloses a method for improving emulsibility and thermal stability of whey protein isolate, and relates to the technical field of protein processing. The preparation method comprises the following specific steps: respectively dissolving whey protein isolate, sodium alginate and steviol glycoside in water, and fully hydrating to obtain a whey protein isolate solution, a sodium alginate solution and a steviol glycoside solution; adjusting the pH values of the three solutions, and mixing according to a certain sequence and proportion to obtain a mixed solution; the obtained mixed solution has good emulsibility and thermal stability, and compared with single WPI, the emulsion stability is increased by 25.2 times. And a practical method is provided for improving the environmental sensitivity and emulsibility of the whey protein isolate.

The application belongs to the technical field of protein processing, and particularly relates to a processing technology of whey protein, which comprises the following steps: subjecting cow milk to homogenization treatment; adding a foaming substance into the homogenized liquid, wherein the foaming substance is bicarbonate and citric acid, and the addition amount of the foaming substance is 3-7% of the mass of fresh cow milk; collecting the supernatant of the cow milk under the condition of high-speed centrifugation at room temperature; and separating and removing casein in the supernatant of the cow milk to obtain whey protein liquid of the cow milk. In the application, the addition of the foaming substance can change the structure of casein colloidal particles, so that casein is separated in large quantities, the purity of the whey protein is improved, a complicated purification step is not needed, the purification cost of the whey protein is reduced, and resources are saved.

The invention relates to the technical field of plant protein processing and extraction, in particular to a protein processing and extracting device which comprises an equipment cabin, the interior of the equipment cabin is divided into an upper cabin body and a lower cabin body through a partition plate, and a centrifugal assembly and a separation assembly are arranged in the equipment cabin; the centrifugal assembly comprises a rotating body arranged in the upper cabin body, a liquid cabin is formed in the middle of the rotating body, an inner gear is mounted in the middle of the rotating body, a mounting part is mounted in the middle of the partition plate, a second motor is mounted at the top end of the mounting part, and a second gear is mounted on an output shaft of the second motor; according to the scheme, the centrifugal assembly containing the staggered sealing strips and the locking assembly and the separation assembly with the one-way liquid passing hole are integrated, and the operation opening and the flushable structure on the periphery of the equipment cabin are matched, so that continuous treatment of soybean protein from alkaline soluble protein, centrifugal impurity removal to acid precipitation protein extraction is achieved; meanwhile, the problems of complicated split operation, easy leakage, impurity reflux and inconvenience in maintenance of the traditional device are solved, and the extraction efficiency and the target protein purity are guaranteed.

The utility model discloses a protein processing flocculation device, including flocculation jar body, the top of flocculation jar body assembly has the jar top cover, and the inside bottom of flocculation jar body assembly is assembled with the water distribution board, the top center of jar top cover is equipped with the driving hole, and is provided with the pivot in the driving hole, the top center of water distribution board assembly has the fixed base, and the bottom of pivot installs in fixed base, and the top of water distribution board is evenly equipped with the through -hole, the outside of pivot is installed with the axle cylinder close to fixed base, both sides of axle cylinder all are installed with the scraper, and the bottom of scraper is installed with the cleaning assembly from left to right equidistance, the setting of this protein processing flocculation device, the structure design is reasonable, through setting cleaning assembly, because the upside of sliding plate is inclined and sets, after the rotation of stirring blade and the resistance of sliding plate, force sliding plate to move downward, make the cleaning rod export the blockage in the through -hole, thereby reach the prevention flocculating material and cause the blockage to the through -hole, thereby influence processing efficiency.

The application belongs to the field of protein processing, and particularly relates to a method for efficiently extracting soybean peptides. The method comprises the following steps: preparing soybean protein into a suspension, and performing ultrasonic pretreatment, then adding a chlorogenic acid grafted UiO-66 biomimetic catalyst, performing catalytic hydrolysis reaction under weak alkaline conditions, and obtaining soybean peptides after separation and purification. The core of the application is that the catalyst innovatively combines the protein recognition and enrichment function of chlorogenic acid with the Zr 6+ hydrolysis active center of UiO-66, significantly improving the efficiency of catalytic hydrolysis of proteins. The yield of soybean peptides obtained by the method is as high as 88.5%, and the catalyst can be recycled for more than 5 times with an activity retention rate of more than 87.5%, greatly reducing the cost. More outstandingly, the obtained soybean peptides have excellent antioxidant activity, and the DPPH free radical scavenging capacity is improved by about 52% compared with the traditional enzyme product, realizing the overall improvement from the production process to the quality of the terminal product.

This invention discloses a subcritical continuous hydrolysis system for proteins, primarily relating to the field of protein processing equipment. It includes a raw material storage tank, a high-temperature pressure reactor, and a product storage tank, sequentially connected by pipelines. The high-temperature pressure reactor is externally equipped with a temperature control jacket, and internally contains a stirring paddle. A discharge valve with pressure monitoring is installed at the top of the reactor; the discharge valve opens when the pressure exceeds a threshold. The discharge valve is connected to a discharge pipeline, which includes one or more heat exchangers. One or more preheaters are located between the raw material storage tank and the high-temperature pressure reactor, and a closed circulation pipeline connects at least one preheater and at least one heat exchanger. The advantages of this invention are: achieving efficient and continuous high-temperature, high-pressure hydrolysis of proteins, improving production efficiency and product uniformity, and significantly reducing production energy consumption.

The invention belongs to the technical field of protein processing, and particularly relates to a purification method and application of lipoprotein lipase. The purification method of the lipoprotein lipase comprises the following steps: inserting a His tag into a coding gene of a lipase specific folding enzyme, preparing the lipase specific folding enzyme with the His tag, carrying out affinity purification, mixing the lipase specific folding enzyme with lipoprotein lipase, incubating, purifying again, and recovering the lipase specific folding enzyme to obtain the lipoprotein lipase. According to the purification method provided by the invention, the harm of a high-salt and high-imidazole environment to the protein in a traditional purification method can be avoided, the problem of protein aggregation in the purification process is solved, mild elution is realized, the inhibition effect of free lifO protein can also be avoided, and a new way is provided for simply, conveniently, efficiently and rapidly purifying the protein.

The utility model relates to the technical field of biological protein processing and production, in particular to a drainage device for protein purification equipment, which comprises a connecting base, a connecting seat, a connecting sleeve, a connecting vertical pipe, a supporting seat, a dense separation net, a molecular sieve filling part, a top net cover, a sampling pipe and a drainage pipe, an opening is formed in the middle of the upper end of the connecting base, the connecting sleeve is fixedly connected to the surface of the edge position of the opening of the connecting base, internal threads are formed in the inner surface of the connecting sleeve, external threads are arranged at the lower end of the connecting vertical pipe, and the lower end of the connecting vertical pipe is in threaded connection with the inner surface of the connecting sleeve. The supporting seat is fixedly connected to the upper surface of the middle position of the connecting base, the dense separation net is connected to the upper end of the supporting seat in a matched mode, and the device has the effects of being high in operation convenience degree, high in filtering efficiency, capable of improving the drainage speed, convenient to clean and not prone to waste.

The invention discloses a method for synergistically improving the solubility of chenopodium quinoa protein based on composite enzymolysis of brown rice starch and pH shift, and belongs to the technical field of chenopodium quinoa protein processing. The method comprises the steps of quinoa protein extraction, brown rice starch extraction, brown rice starch composite enzymolysis, pH deviation treatment, 0.1 mol / L HCL callback, freeze drying and the like. Various amylolytic enzymes such as pullulanase and alpha-amylase and a compounding scheme are screened, and process parameters are optimized, so that the solubility of the modified quinoa protein reaches 88.94%-92.15%, the absolute value of the Zeta potential is 20-30 mV, and functional characteristic differentiation is adaptive to different food scenes. The composite enzymolysis and pH shift synergistic effect mechanism is clear, the process is stable and repeatable, the problems of limited modification effect and complex operation in the prior art are solved, and the method has important industrial application value.

The application discloses a kind of cottonseed protein processing technology of desolventizing and dephenolization, and relates to the technical field of cottonseed processing.The application comprises the following steps: S1, cottonseed pretreatment;S2, cottonseed cotton oil removal treatment;S3, cottonseed dephenolization treatment;S4, wet meal is treated, and a large amount of solvent in wet meal is separated by the cooperation of evaporation tower and drying tower;S5, wet meal is treated, and wet meal is desolventized and dried by gradually increasing temperature in stages.The application provides a kind of processing technology for cottonseed protein, which is dephosphorized and dephenolized, and residual solvent is removed, so that cottonseed protein can be obtained as edible material, and the utilization rate of cottonseed is improved.

Embodiments of the present application disclose a protein processing method and device, computer equipment and a storage medium, belonging to the technical field of computer. The method comprises: displaying an input first protein sequence and a mutation statement, the mutation statement indicating mutation of the first protein sequence, the mutation statement comprising at least one mutation instruction, the mutation instruction comprising a mutation position and a mutation mode; in response to a processing request for the first protein sequence and the mutation statement, for each mutation instruction in the mutation statement, mutating the mutation position in the first protein sequence according to the mutation mode in the mutation instruction to obtain a second protein sequence; and displaying the second protein sequence. The protein sequence is mutated based on the mutation statement, the process of protein mutation is simulated, the mutated protein is obtained, and thus the process of mutating the protein is simplified, the efficiency of mutating the protein is improved, and the efficiency of protein processing is further improved.

The utility model discloses a multistage efficient filtering device for earthworm protein, which relates to the technical field of earthworm protein processing equipment and comprises a feed hopper, a support frame is mounted in the feed hopper, a protective sleeve is mounted at the top of the support frame, and a power motor is mounted in the protective sleeve. According to the earthworm protein filtering device, the limiting rod, the limiting spring, the secondary filtering sleeve, the corrugated sealing sleeve, the fixing shaft, the fixing sleeve and the primary filtering sleeve are arranged, the corrugated sealing sleeve is in threaded connection with the secondary filtering sleeve and the primary filtering sleeve through the fixing shaft and the fixing sleeve respectively for sealing, the situation that earthworm protein is scattered in the filtering process and waste is caused is avoided, and meanwhile the earthworm protein filtering device is convenient to use. Through the arrangement of the second-stage filter sleeve and the first-stage filter sleeve, the earthworm protein powder can be subjected to multi-stage fine filtration, the purity and the quality of the earthworm protein are improved, the second-stage filter sleeve can be pulled to do vertical reciprocating motion in the filtration process, the earthworm protein powder on the surface of the second-stage filter sleeve is vibrated, and the filtration efficiency is improved.

The invention discloses a synergistic modification method of a soybean protein-casein compound and a product, and belongs to the technical field of food protein processing and modification.The method comprises the steps that SPI and CS are mixed according to the mass ratio selected from (9: 1) to (1: 9) and dissolved in water, dispersion liquid with the concentration being 4%-8% is prepared, the pH is adjusted to 10-12.0 after hydration, and stirring is conducted; carrying out ultrasonic treatment on the protein liquid under the condition of keeping the alkaline pH unchanged; finally, the pH is adjusted back to be neutral, centrifugation is conducted, and supernate is taken to obtain the modified compound. According to the method, an ultrasonic cavitation effect acts on protein molecules which are expanded in an alkaline manner to generate a synergistic effect, and protein conformation is induced to generate flexible rearrangement, so that the solubility, emulsification, foaming and other interface functions of the compound are synergistically and remarkably improved. The invention provides a green and efficient way for constructing a high-performance plant-animal double-protein ingredient.

The invention discloses white kidney bean protein as well as a preparation method and application thereof in the field of gel, and belongs to the technical field of protein processing. The white kidney bean protein is specifically extracted by adopting a buffer solution as an extracting agent and adopting a sulfuric acid precipitation method, and the obtained white kidney bean protein is complete in structure and high in activity; compounding the white kidney bean protein with fish skin gelatin, and performing heat treatment to obtain white kidney bean protein fish skin gel; the gel is milky white and has no obvious flowing phenomenon after inclining; in addition, the water holding capacity and the gel property are relatively high; good application prospects are realized in the fields of medicine / cell carriers, wound dressings, food processing, cosmetics and the like; the method not only improves the functional characteristics of the white kidney bean protein, but also enhances the gel performance of the fish skin gelatin, and widens the idea for improving the stability of the novel plant protein processing modification field.

The invention belongs to the technical field of edible protein processing, and discloses fermentation cottonseed protein hydrolysate concentrating and drying equipment which comprises a synchronous processing barrel assembly, a traction material squeezing arm assembly used for squeezing and discharging hydrolysate is arranged in a processing straight barrel, and the synchronous processing barrel assembly is connected with the traction material squeezing arm assembly. In the concentrating and drying process, the traction extrusion arm assembly is always in the state of extruding the interior of the treatment straight barrel, raw materials in the treatment straight barrel can be subjected to concentration treatment and drying treatment at the same time, and the concentration and drying efficiency is improved. Concentration treatment and drying treatment can be carried out synchronously, the trouble of frequent material pouring caused by separation of traditional working procedures is avoided, meanwhile, dynamic homogenization of hydrolysate is achieved through circular rotation and autorotation of the ultrasonic head, and then the homogenization effect of the materials in the treatment straight barrel during concentration and drying and the homogenization consistency of all positions are guaranteed.

The application provides a method for improving the stability and solubility of fish myofibrillar protein, and belongs to the technical field of aquatic protein processing. The method comprises the following steps: mixing fish myofibrillar protein solution with enzymes and carrying out enzymolysis; the enzymes include one or more of glutamine enzyme, protein glutamine enzyme, chymotrypsin and papain. The method provided by the application improves the stability and low-salt solubility of fish myofibrillar protein.

The utility model discloses an earthworm protein filtering and purifying machine, which relates to the technical field of earthworm protein processing equipment and comprises a base, a vibration pump is mounted on the outer wall of the base, a separation bin is mounted at the top of the base, a discharge port is formed in the side wall of the separation bin, a fine-mesh filter screen is mounted on the inner wall of the separation bin, and a filter screen is mounted on the fine-mesh filter screen. And a stirring rod is mounted on the inner wall of the fine-mesh filter screen. Through the arrangement of the base, the vibration pump, the separation bin, the discharge hole, the fine-mesh filter screen, the stirring rod and the rotating motor, the rotating motor is started to drive the stirring rod to rotate, so that an earthworm protein solution entering the separation bin is centrifugally rotated and is centrifugally filtered through the fine-mesh filter screen, the operation is convenient and fast, and the working efficiency is improved. Meanwhile, a vibration pump is arranged, residues, solid particles and the like filtered on the inner wall of the fine-mesh filter screen can be shaken off, and the situation that the inner wall of the fine-mesh filter screen is blocked, and the filtering effect is affected is avoided.

The invention relates to the technical field of protein processing, and discloses enzymolysis modification of foreign protein and application of the foreign protein in baked foods.The method comprises the steps that before an enzymolysis reaction starts, the foreign protein, proteolytic enzyme, reducing sugar in a specific proportion and food-grade non-reducing polysaccharide are mixed in advance; according to the preparation method, a reducing sugar is added, so that new amino groups generated by cutting off peptide bonds through enzymolysis are covalently anchored by the reducing sugar at the moment of generation, and meanwhile, the chemical anchoring rate is always higher than the physical aggregation rate through physical retardation formed by diffusion of polysaccharide to hydrophobic peptide fragments, and the traditional process route of first degradation and then repair is avoided; the degradation process of the protein is converted into an integrated process which is synchronously carried out with functional modification of the peptide fragment, so that the problems of aggregation and bitter taste of the product are avoided from a molecular source, and the functional protein hydrolysate which can be directly applied to the field of baked foods is obtained.

The invention belongs to the field of protein processing, and particularly relates to a method for efficiently extracting soybean peptide. The method comprises the following steps: preparing soybean protein into a suspension, performing ultrasonic pretreatment, adding a chlorogenic acid grafted UiO-66 biomimetic catalyst, performing catalytic hydrolysis reaction under a weakly alkaline condition, and performing separation and purification after the reaction to obtain the soybean peptide. The core of the invention lies in that the catalyst innovatively combines the protein recognition and enrichment functions of chlorogenic acid with the Zr6 + hydrolytic activity center of UiO-66, and significantly improves the efficiency of catalytic hydrolysis of protein. According to the method, the yield of the soybean peptide reaches up to 88.5%, the catalyst can be recycled for more than five times, the activity retention rate exceeds 87.5%, and the cost is greatly reduced. More prominently, the prepared soybean peptide has excellent antioxidant activity, the DPPH free radical scavenging capacity of the soybean peptide is improved by about 52% compared with that of a traditional enzyme method product, and comprehensive improvement from the production process to the quality of a terminal product is achieved.

The invention belongs to the technical field of protein processing, and particularly relates to a preparation method and application of a food-grade hydrolyzed keratin product, the preparation method comprises the following steps: S1, sequentially carrying out first enzymolysis treatment and second enzymolysis treatment on a keratin-containing raw material to obtain an enzymatic hydrolysate; s2, the enzymatic hydrolysate is subjected to decoloration and deodorization treatment, and a decoloration solution is obtained; and S3, sequentially carrying out ultrafiltration treatment and nanofiltration treatment on the decolored solution to obtain a concentrated solution containing the food-grade hydrolyzed keratin product. The preparation method combines multiple processes of two-stage enzymolysis, decoloration and deodorization, ultrafiltration screening and nanofiltration concentration, has the advantages of being green, safe, simple in process, easy for large-scale production and the like, further, the food-grade hydrolyzed keratin product prepared by the method has the advantages of being high in purity, capable of being completely dissolved in water, low in sodium, free of odor and peculiar smell and the like, and meanwhile, the food-grade hydrolyzed keratin product is suitable for industrial production. And the keratin is also rich in cystine with important nutrition and physiological activity in keratin.

This invention discloses a green extraction and preparation method for Chlorella protein, belonging to the field of protein processing. The method includes: pre-treatment of Chlorella protein-producing cells by ball milling to disrupt cell walls; extraction using a microwave-assisted deep eutectic solvent; separation by water extraction; and drying to obtain Chlorella protein. This method can directly produce high-purity Chlorella protein, and the resulting product possesses excellent physicochemical properties, functional characteristics, and biological activity. This invention provides solid technical support for the green and efficient extraction and application of Chlorella protein.

The invention relates to the technical field of pharmaceutical chemicals, and discloses a thienopyrimidine derivative, a preparation method thereof and application of the thienopyrimidine derivative in killing trichomonad vaginalis. The derivative is synthesized by taking a thiophene ring and a pyrimidine ring which are fused as a parent nucleus and carrying a specific substituent and a representative compound G-18 through a multi-step reaction. The trichomonas vaginalis can destroy the structure and function of a trichomonas vaginalis body by regulating and controlling the metabolism of trichomonas vaginalis, protein processing and other key channels, the trichomonas vaginalis can be completely killed within 24 hours at the concentration of 10 micrograms / mL, the MIC is 4 micrograms / mL, the IC50 is 1 microgram / mL, and the toxicity to LX-2 and 293T cells is low. The compound is used for treating trichomonad vaginalis (containing metronidazole drug-resistant strains) for the first time, a rapid and efficient CCK-8 high-throughput screening technology is established, the drug-resistant treatment blank is filled up, and brand new drug candidates and technical supports are provided.

The application discloses a corn heat shock transcription factor ZmHsf05 gene in improving corn inbred pollen viability, ear grain number and single ear grain weight. ZmHsf05 Firstly, a plant overexpression vector containing the gene is constructed to obtain a corn material overexpressing the gene. ZmHsf05 Through continuous backcrossing and selfing, an improved corn inbred overexpressing the gene is obtained. ZmHsf05 The pollen viability, ear grain number and single ear grain weight of the improved inbred are significantly improved. ZmHsf05 Through pollen transcriptome sequencing, it is found that ZmHsf05 improves pollen viability by regulating target genes in endoplasmic reticulum protein processing, cysteine and methionine metabolism and flavonoid biosynthesis pathways. Therefore, the improved corn inbred overexpressing the gene has good breeding potential in production.