60 results about "Protein processing" patented technology

The invention belongs to the technical field of cereal protein processing, and particularly relates to wheat gliadin with good color and luster and glutenin with high solubility as well as a preparation method and application of the wheat gliadin and the glutenin. According to the method, a cold water solubility control coupling one-step freezing purification technology is broken through, the concentration of ethanol and the temperature of the solution are controlled through cold water, the dissolving state and aggregation behavior of wheat gliadin are synchronously regulated and controlled, then freezing treatment and a one-step method are coupled to achieve controllable protein aggregation, precipitation and freezing, and the purposes of separation and purification are achieved. The technology is green, efficient, simple, convenient and energy-saving, the obtained alcohol-soluble protein is good in color and luster, and industrial production is easy to realize. Furthermore, a membrane separation coupling high-pressure microjet technology is broken through, and on the basis of a technology for obtaining a glutenin concentrated solution through membrane separation, the protein concentrated solution is directly treated by adopting an alkaline condition synchronous high-pressure microjet circulating modification method, so that the structural characteristics such as the scale and the surface hydrophobicity of the target protein and the aggregation behavior are further regulated and controlled; the glutenin is endowed with better solubility.

The invention belongs to the technical field of protein processing, and particularly relates to modified beta-lactoglobulin as well as a preparation method and application thereof. The modified beta-lactoglobulin is a composite modified product which is obtained by performing a papain hydrolysis treatment process on beta-lactoglobulin to obtain a hydrolysate and then performing glycosylation modification on the hydrolysate by adopting chitosan oligosaccharide and transglutaminase. The product has significantly improved oxidation resistance, emulsifying properties and foaming activity, and is suitable as a food processing ingredient, such as an antioxidant, an emulsifier or a foaming agent.

The invention discloses a high-emulsification-activity gluten protein based on irradiation modification and a preparation method thereof in the field of wheat protein processing, and the method comprises the following steps: dispersing wheat gluten protein in deionized water, stirring to form a uniform suspension, adjusting the pH value, carrying out enzymolysis treatment on the suspension by using alkaline protease, and drying to obtain the high-emulsification-activity gluten protein. Centrifugally drying to obtain primary protein powder; dissolving the protein primary powder in an irradiation solvent to obtain a protein dispersion liquid, and irradiating the protein dispersion liquid by using cobalt-60 gamma rays as an irradiation source in an intermittent manner; and taking the irradiated protein dispersion liquid, and performing centrifugal drying to obtain the high-emulsification active gluten protein. The method comprises the following steps: firstly, carrying out limited hydrolysis on gluten protein by using protease to generate a smaller peptide fragment, so that the irradiation efficiency and the emulsion stability are improved; by adopting an intermittent irradiation treatment mode, protein aggregation caused by heat effect can be reduced, the emulsifying effect is improved, and the stability and adhesive force of an adhesive can be improved when the emulsion is applied to the adhesive.

The application discloses an inhibitor for inhibiting KRAS phase separation to play an anti-tumor role and application, and relates to the fields of tumor molecular biology and targeted therapy.The application aims at solving the problems of limited effect, strong drug resistance and limited application range of KRAS tumor treatment methods, and the inhibitor is a statin.The KRAS protein can form a liquid condensate in cells, and the application discloses the mechanism of the KRAS protein in promoting KRAS protein processing, transportation and downstream signal activation, and further provides a technical scheme for inhibiting tumor growth and enhancing the therapeutic effect of a KRAS inhibitor by inhibiting the formation of KRAS condensates, so as to provide a new target and intervention approach for tumor treatment.

The present invention relates to a method for producing a plant-derived protein processed food, including swelling a porous granular plant-derived protein with water or a seasoning liquid, and then adding a powdered plant-derived protein and transglutaminase and kneading the mixture, and a plant-derived protein processed food containing a composition containing a porous granular plant-derived protein and a powdered plant-derived protein, the composition being acted on by transglutaminase. The present invention makes it possible to provide a plant-derived protein processed food having a texture equivalent to that of meat, particularly steak meat or sliced meat.

The invention discloses a method for preparing protein concentrate from high-denaturation oilseed meal, and belongs to the technical field of edible protein processing. The method comprises the following steps: crushing and screening to obtain meal powder with different protein contents by utilizing the distribution rule of proteins and structural carbohydrates in the microstructure of the high-denaturation oilseed meal and the physical property characteristics of components, and carrying out graded processing treatment according to the property difference of the meal powder; an air-electric field separation device is used for treating large-particle oilseed meal, high-fiber components which are difficult to remove in a traditional process are separated out, and high-protein components with solubility (NSI) similar to that of low-denaturation soybean meal and high gel property are obtained; proper concentration or alcoholase treatment is selected according to the protein content, denaturation degree and structural characteristics of the screening component, and a concentrated protein product meeting specific protein content requirements and / or functional characteristics can be obtained.

The invention belongs to the field of feed protein processing, and particularly relates to a method for improving the bioavailability of animal feed protein through chemical enzymolysis. The method comprises the following steps: mixing a compound protein raw material with water to form a suspension; sodium tripolyphosphate is added for phosphorylation pretreatment, so that protein is subjected to specific modification; after the pH is adjusted to be acidic, pepsase and acid phosphatase are added at the same time for an enzymolysis reaction; and after the reaction is finished, performing enzyme deactivation and drying to obtain the modified feed protein. According to the method, a specific enzyme cutting site is created on a protein molecule through chemical phosphorylation, then directional hydrolysis is achieved through efficient recognition of pepsin on the site, and meanwhile dynamic regulation and control of the hydrolysis process are achieved by means of the dephosphorylation effect of acid phosphatase. The obtained product has the outstanding advantages of high content of small peptides, good digestive absorbability, low bitterness, strong biological activity and the like, and the bioavailability of feed protein is remarkably improved.

The invention discloses a collagen peptide composition with a function of increasing muscle strength and application of the collagen peptide composition. The collagen peptide composition comprises fish collagen peptide and beef tendon collagen peptide. A mouse senescence sarcopenia model is constructed, then a collagen peptide composition is used for intragastric intervention, and the improvement effect is evaluated through behavioral testing, body component analysis and HE staining. The potential target and regulatory pathway of the collagen peptide composition are explored by combining network pharmacology and molecular docking. Results show that the four-limb grip strength, treadmill exhaustion time and movement distance of a model mouse can be remarkably improved, quadriceps femoris muscle and gastrocnemius muscle indexes are recovered, and muscle fiber atrophy and collagen deposition are relieved. And it is found that TNF, IL1B, AKT1, mTOR and MAPK1 may be core targets of the TNF, IL1B, AKT1, mTOR and MAPK1. The polypeptide plays a role by regulating and controlling biological processes such as MAPK cascade, protein processing and inflammatory reaction, cell apoptosis, a cAMP pathway and a calcium signal pathway.

The utility model relates to the technical field of earthworm protein processing, in particular to a multifunctional liquid nitrogen device for earthworm protein production, which comprises a processing cabinet, a cabinet door, a blanking hopper, a hopper plug, a liquid inlet, a connecting pipe, a liquid nitrogen spray head and the like, a cabinet door is rotationally connected to one side of the treatment cabinet, a discharging hopper is fixedly connected to the top of the treatment cabinet, a hopper plug used for sealing an opening of the discharging hopper is movably connected to the discharging hopper, a hopper neck of the discharging hopper extends into the treatment cabinet and is provided with a liquid nitrogen spray head, a liquid inlet is formed in the position, close to the discharging hopper, of the treatment cabinet, and the liquid inlet is communicated with the liquid nitrogen spray head through a connecting pipe. According to the quick-freezing and grinding device, through cooperation of the liquid nitrogen spray head, the grinding frame, the grinding rod, the driving mechanism and other parts, tight connection of quick-freezing work and grinding work is achieved, then the wind direction of degradation and denaturation caused by raw material transfer is avoided, and the production efficiency and the product quality are effectively improved.

The invention belongs to the technical field of corn protein processing, and particularly relates to corn enzymolysis protein with high protein content and high micromolecule active substances and a preparation method. The method comprises the following steps: S1, weighing corn protein powder, adding water into the corn protein powder, stirring, adding alkali to adjust the pH value, and then adding medium-temperature amylase for enzymolysis; s2, separating the materials to obtain filter residues; s3, adding water into the materials, stirring, and adding acidic cellulase for enzymolysis; and S4, raising the temperature of the material, adjusting the pH value with alkali, and adding alkaline protease for enzymolysis. The corn enzymolysis protein prepared by the method can be used as a high-quality functional protein to be applied to aquatic feed, so that the intestinal and liver health of aquatic animals is remarkably improved, the total dosage of protein raw materials in the aquatic feed is reduced, and the dosage of imported protein resources (such as fish meal) is reduced; pigmentation can be promoted, and the commodity performance of aquatic animals can be improved; therefore, the economic benefits of aquatic feed enterprises and farmers are improved.

The invention relates to the technical field of silk protein processing equipment, in particular to a hydrolyzed silk protein preparation method and an integrated reaction device.The integrated reaction device comprises a workbench, the workbench is provided with a cleaning unit, a drying unit, a smashing and separating unit and a hydrolyzed silk protein preparation integrated unit, and the cleaning unit comprises a cleaning tank; an ultrasonic generator is arranged on the inner wall of the cleaning tank, a water drainage cavity is formed in the bottom of the cleaning tank, a water suction pump is arranged in the water drainage cavity, the units for cleaning, drying, smashing and separating and hydrolyzed silk protein preparing are integrated on the workbench, and the occupied area of the equipment is reduced; automatic material transfer is achieved among the units through auxiliary assemblies, the repeated transfer link of hydrolyzed silk among different processing containers is omitted, the manual operation intensity is reduced, meanwhile, the whole process of hydrolyzed silk protein preparation is integrated, the production process is safe, simple, convenient and economical, and industrial large-scale production is easy to achieve.

The invention provides an earthworm protein concentration and purification device, and relates to the technical field of earthworm protein processing. A feeding hopper is arranged at a feeding opening in the top of the conveying cylinder, a first motor is installed outside the conveying cylinder, a spiral conveying shaft is connected into the conveying cylinder, and a driving shaft of the first motor is connected with the spiral conveying shaft. A stress piece is installed outside the conveying cylinder, and a baffle is connected into the stress piece. According to the invention, the double springs are arranged, so that the ejection resistance of a stressed part is effectively increased, and the content of earthworm protein liquid in earthworm materials is effectively reduced; and the earthworm protein liquid entering the liquid discharge cavity is filtered through the filter screen II, so that small-particle earthworm residues contained in the earthworm protein liquid are removed, and the impurity removal and purification effects of the earthworm protein liquid are guaranteed. The problems that in the application process of an existing earthworm protein concentration and purification device, only part of earthworm protein liquid can be obtained, much earthworm protein liquid is still reserved in earthworm materials, and waste is caused are solved.

The invention discloses a method for improving emulsibility and thermal stability of whey protein isolate, and relates to the technical field of protein processing. The preparation method comprises the following specific steps: respectively dissolving whey protein isolate, sodium alginate and steviol glycoside in water, and fully hydrating to obtain a whey protein isolate solution, a sodium alginate solution and a steviol glycoside solution; adjusting the pH values of the three solutions, and mixing according to a certain sequence and proportion to obtain a mixed solution; the obtained mixed solution has good emulsibility and thermal stability, and compared with single WPI, the emulsion stability is increased by 25.2 times. And a practical method is provided for improving the environmental sensitivity and emulsibility of the whey protein isolate.

The application belongs to the technical field of protein processing, and particularly relates to a processing technology of whey protein, which comprises the following steps: subjecting cow milk to homogenization treatment; adding a foaming substance into the homogenized liquid, wherein the foaming substance is bicarbonate and citric acid, and the addition amount of the foaming substance is 3-7% of the mass of fresh cow milk; collecting the supernatant of the cow milk under the condition of high-speed centrifugation at room temperature; and separating and removing casein in the supernatant of the cow milk to obtain whey protein liquid of the cow milk. In the application, the addition of the foaming substance can change the structure of casein colloidal particles, so that casein is separated in large quantities, the purity of the whey protein is improved, a complicated purification step is not needed, the purification cost of the whey protein is reduced, and resources are saved.

The utility model discloses a solid-liquid separation device for earthworm protein processing, and relates to the technical field of earthworm protein processing, the solid-liquid separation device comprises a collection cylinder, a liquid guide pipe is rotatably arranged in the middle of the top end of the collection cylinder, the liquid guide pipe is driven by a driving mechanism to rotate, a liquid guide cylinder is arranged at the top end of the liquid guide pipe, a separation cylinder is arranged in the liquid guide cylinder, and a liquid outlet is formed in the separation cylinder. Through holes are formed in the bottom end and the bottom of the side face of the separation barrel, and a material pressing mechanism is arranged at the top end of the separation barrel; the earthworm protein solid-liquid separation device has the advantages that the driving mechanism can drive the separation barrel to rotate quickly, so that solid-liquid separation is performed on earthworm protein, solids are reserved in the separation barrel, the separated solids can be compressed by the aid of the pressing mechanism, liquid attached to the surfaces of the solids can be discharged, and the earthworm protein solid-liquid separation device is simple in structure and convenient to use. And under the action of a liquid guide barrel and a liquid guide pipe, the liquid is discharged into a collecting barrel to be collected.

The invention relates to the technical field of plant protein processing and extraction, in particular to a protein processing and extracting device which comprises an equipment cabin, the interior of the equipment cabin is divided into an upper cabin body and a lower cabin body through a partition plate, and a centrifugal assembly and a separation assembly are arranged in the equipment cabin; the centrifugal assembly comprises a rotating body arranged in the upper cabin body, a liquid cabin is formed in the middle of the rotating body, an inner gear is mounted in the middle of the rotating body, a mounting part is mounted in the middle of the partition plate, a second motor is mounted at the top end of the mounting part, and a second gear is mounted on an output shaft of the second motor; according to the scheme, the centrifugal assembly containing the staggered sealing strips and the locking assembly and the separation assembly with the one-way liquid passing hole are integrated, and the operation opening and the flushable structure on the periphery of the equipment cabin are matched, so that continuous treatment of soybean protein from alkaline soluble protein, centrifugal impurity removal to acid precipitation protein extraction is achieved; meanwhile, the problems of complicated split operation, easy leakage, impurity reflux and inconvenience in maintenance of the traditional device are solved, and the extraction efficiency and the target protein purity are guaranteed.

The utility model discloses a protein processing flocculation device, including flocculation jar body, the top of flocculation jar body assembly has the jar top cover, and the inside bottom of flocculation jar body assembly is assembled with the water distribution board, the top center of jar top cover is equipped with the driving hole, and is provided with the pivot in the driving hole, the top center of water distribution board assembly has the fixed base, and the bottom of pivot installs in fixed base, and the top of water distribution board is evenly equipped with the through -hole, the outside of pivot is installed with the axle cylinder close to fixed base, both sides of axle cylinder all are installed with the scraper, and the bottom of scraper is installed with the cleaning assembly from left to right equidistance, the setting of this protein processing flocculation device, the structure design is reasonable, through setting cleaning assembly, because the upside of sliding plate is inclined and sets, after the rotation of stirring blade and the resistance of sliding plate, force sliding plate to move downward, make the cleaning rod export the blockage in the through -hole, thereby reach the prevention flocculating material and cause the blockage to the through -hole, thereby influence processing efficiency.

The utility model provides a multi-stage screening machine for natto protein processing, and relates to the technical field of natto processing. The utility model is used to solve the technical problem that the screening machine provided by the existing patent pours the raw materials into the chassis at one time, which is easy to cause blockage and jamming, and is not conducive to the crushing and screening of natto protein. A multi-stage screening machine for natto protein processing includes a support frame, a screening box, a feed hopper, a graded screening component and a discharge hopper. The support frame is equipped with a screening box, the top of the screening box is equipped with a feed hopper, the bottom of the screening box is equipped with a discharge hopper, and the screening box is provided with a graded screening component for screening natto protein. By pouring the natto protein raw material into the feed hopper, the raw material falls on the feeding roller, and the servo motor is started to drive the feeding roller to rotate, so that the natto protein raw material is intermittently fed into the screening box without causing blockage and jamming, thereby achieving the effect of intermittent feeding.

The present invention provides a soy protein emulsion gel product and a preparation method thereof, belonging to the technical field of plant protein processing. Using soy protein as raw material, the invention first enzymatically hydrolyzes and heat-treats soy protein to obtain soy protein with different 7S / 11S ratios and soluble aggregate contents. Oil is then added and emulsified to prepare a soy protein emulsion. On this basis, glucono-δ-lactone is used to prepare an acid-induced emulsion gel. This method achieves the preparation of a soy protein emulsion gel with excellent textural properties and microstructure, broadening the application of soy protein in food and related fields.

The application belongs to the field of protein processing, and particularly relates to a method for efficiently extracting soybean peptides. The method comprises the following steps: preparing soybean protein into a suspension, and performing ultrasonic pretreatment, then adding a chlorogenic acid grafted UiO-66 biomimetic catalyst, performing catalytic hydrolysis reaction under weak alkaline conditions, and obtaining soybean peptides after separation and purification. The core of the application is that the catalyst innovatively combines the protein recognition and enrichment function of chlorogenic acid with the Zr 6+ hydrolysis active center of UiO-66, significantly improving the efficiency of catalytic hydrolysis of proteins. The yield of soybean peptides obtained by the method is as high as 88.5%, and the catalyst can be recycled for more than 5 times with an activity retention rate of more than 87.5%, greatly reducing the cost. More outstandingly, the obtained soybean peptides have excellent antioxidant activity, and the DPPH free radical scavenging capacity is improved by about 52% compared with the traditional enzyme product, realizing the overall improvement from the production process to the quality of the terminal product.

The invention provides a pea protein isolate functionality improving method and a pea protein isolate product, and relates to the technical field of edible protein processing. The method provided by the invention comprises the following steps: stirring and mixing edible polysaccharide into a pea protein isolate hydrated solution; the functionality comprises at least one of solubility, foamability and emulsibility. According to the invention, the edible polysaccharide is added into the pea protein isolate hydrated solution, and the protein can interact with the polysaccharide to generate a compound or colloid structure, so that the foamability, emulsibility and solubility of the pea protein isolate are effectively improved.

The present invention discloses a fully automatic recovery device based on wheat protein, which includes a recovery pool, a washing water discharge pipeline, a granular wheat protein filtration component, a main wheat protein collection pipe, a fluffy wheat protein filtration component, a wheat protein collection branch pipe, and a liquid discharge pipeline; a washing water discharge pipeline is provided at the upper end of the recovery pool, a granular wheat protein filtration component is provided below the washing water discharge pipeline, at least one group of fluffy wheat protein filtration components is provided in the recovery pool, and a liquid discharge pipeline for discharging the filtered washing water is provided at the end in the recovery pool. By arranging a granular wheat protein filtration component and a fluffy wheat protein filtration component in the recovery pool, the present invention effectively separates the broken wheat protein in the water body, realizes the recycling of granular and fluffy wheat protein, improves the utilization rate of production raw materials, and reduces the production cost during wheat protein processing.

This invention discloses a subcritical continuous hydrolysis system for proteins, primarily relating to the field of protein processing equipment. It includes a raw material storage tank, a high-temperature pressure reactor, and a product storage tank, sequentially connected by pipelines. The high-temperature pressure reactor is externally equipped with a temperature control jacket, and internally contains a stirring paddle. A discharge valve with pressure monitoring is installed at the top of the reactor; the discharge valve opens when the pressure exceeds a threshold. The discharge valve is connected to a discharge pipeline, which includes one or more heat exchangers. One or more preheaters are located between the raw material storage tank and the high-temperature pressure reactor, and a closed circulation pipeline connects at least one preheater and at least one heat exchanger. The advantages of this invention are: achieving efficient and continuous high-temperature, high-pressure hydrolysis of proteins, improving production efficiency and product uniformity, and significantly reducing production energy consumption.

The invention provides a modified casein-polyphenol compound as well as a preparation method and application thereof, and belongs to the technical field of protein processing. The invention provides the application of the small red garlic polyphenol in casein modification for the first time, and further provides a preparation method of a modified casein-small red garlic polyphenol compound, and the preparation method comprises the following steps: preparing a casein culture solution, inoculating casein casei for fermentation, and sterilizing after fermentation to obtain a fermentation solution; mixing the small red garlic polyphenol with the fermentation liquor for reaction, and drying a reaction product to obtain the modified casein-small red garlic polyphenol covalent complex. The stability, the oxidation resistance, the solubility, the emulsifying capacity, the emulsifying stability, the foamability and the foaming stability of the casein can be obviously improved, and the method has a wide application prospect in the field of protein processing.

The invention belongs to the technical field of protein processing, and particularly relates to a purification method and application of lipoprotein lipase. The purification method of the lipoprotein lipase comprises the following steps: inserting a His tag into a coding gene of a lipase specific folding enzyme, preparing the lipase specific folding enzyme with the His tag, carrying out affinity purification, mixing the lipase specific folding enzyme with lipoprotein lipase, incubating, purifying again, and recovering the lipase specific folding enzyme to obtain the lipoprotein lipase. According to the purification method provided by the invention, the harm of a high-salt and high-imidazole environment to the protein in a traditional purification method can be avoided, the problem of protein aggregation in the purification process is solved, mild elution is realized, the inhibition effect of free lifO protein can also be avoided, and a new way is provided for simply, conveniently, efficiently and rapidly purifying the protein.

The application discloses a kind of cavitation ultrasonic microwave synergic whey protein microparticulation equipment, belong to protein processing equipment technical field;The equipment includes sequentially communicating feed system, hydrodynamic cavitation component, outer wall focused ultrasound component, microwave temperature control shaping component, online high shear component, discharge system and control system;Low-pressure bubble formation and pressure recovery collapse area are formed by hollow cavity, sudden expansion release cavity and first back pressure valve, focused ultrasound transducer is set to corresponding pipe wall outside, and its acoustic focus is located in cavitation bubble collapse or pressure recovery area.The material output by outer wall focused ultrasound component directly enters the single-layer spiral wave-transparent medium tube of microwave temperature control shaping component, and the control system adjusts microwave power according to outlet temperature feedback, and the rear online high shear component limits secondary aggregation after shaping.The equipment is used to coordinate the nucleation, depolymerization and shaping process of whey protein microparticles in continuous flow state, reduce excessive aggregation and pipeline fouling, and improve continuous operation stability.

The utility model relates to the technical field of biological protein processing and production, in particular to a drainage device for protein purification equipment, which comprises a connecting base, a connecting seat, a connecting sleeve, a connecting vertical pipe, a supporting seat, a dense separation net, a molecular sieve filling part, a top net cover, a sampling pipe and a drainage pipe, an opening is formed in the middle of the upper end of the connecting base, the connecting sleeve is fixedly connected to the surface of the edge position of the opening of the connecting base, internal threads are formed in the inner surface of the connecting sleeve, external threads are arranged at the lower end of the connecting vertical pipe, and the lower end of the connecting vertical pipe is in threaded connection with the inner surface of the connecting sleeve. The supporting seat is fixedly connected to the upper surface of the middle position of the connecting base, the dense separation net is connected to the upper end of the supporting seat in a matched mode, and the device has the effects of being high in operation convenience degree, high in filtering efficiency, capable of improving the drainage speed, convenient to clean and not prone to waste.

The invention relates to a method for improving emulsifying property of soybean protein isolate by combined treatment of enzymolysis and oxidized carboxymethyl cellulose, which belongs to the technical field of protein processing and comprises the following steps: (1) enzymolysis treatment of soybean protein isolate (SPI); (2) preparing oxidized carboxymethyl cellulose (OCMC); and (3) respectively stirring and mixing the SPH and the SPI with the OCMC, and storing at 4 DEG C overnight to obtain SPH-OCMC and SPI-OCMC compounds. According to the modification method disclosed by the invention, the soybean protein isolate with good emulsifying property can be obtained, the emulsifying activity is improved by 63.64%, the emulsifying stability is improved by 1.82 times, the application value of the soybean protein is further expanded, and the modification method has very important significance in expanding the application range and prospect of the soybean protein in the fields of food industry and the like and has a wide application prospect. Meanwhile, the method has important strategic significance for promoting the upgrading of a soybean agricultural product deep processing technology.

The invention discloses a method for synergistically improving the solubility of chenopodium quinoa protein based on composite enzymolysis of brown rice starch and pH shift, and belongs to the technical field of chenopodium quinoa protein processing. The method comprises the steps of quinoa protein extraction, brown rice starch extraction, brown rice starch composite enzymolysis, pH deviation treatment, 0.1 mol / L HCL callback, freeze drying and the like. Various amylolytic enzymes such as pullulanase and alpha-amylase and a compounding scheme are screened, and process parameters are optimized, so that the solubility of the modified quinoa protein reaches 88.94%-92.15%, the absolute value of the Zeta potential is 20-30 mV, and functional characteristic differentiation is adaptive to different food scenes. The composite enzymolysis and pH shift synergistic effect mechanism is clear, the process is stable and repeatable, the problems of limited modification effect and complex operation in the prior art are solved, and the method has important industrial application value.