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Processing technology for extracting protein by broken rice enzyme method

A processing technology, broken rice enzymatic method, applied in the field of broken rice enzymatic extraction protein processing technology, can solve the problems of low price of broken rice, low level of comprehensive utilization, low protein quality, low extraction rate, etc., to achieve full realization Effects of utilizing, improving solubility, and reducing energy consumption

Inactive Publication Date: 2013-01-16
安徽省旌德县辉煌粮油有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technique uses specific chemicals called proteolases that break down or altered rice's albumin molecules making them easier to dissolve when mixed with other materials like milk powder during processing. These techniques help improve the efficiency at producing high-quality breaking wheat products while reducing costs associated therewith.

Problems solved by technology

The technical problem addressed by this patented method relates to improving the efficiency at producing valuable protease containing products like peptides or amino acids (peptide) that can be used as ingredients in various applications including healthcare nutritional supplementation and cosmetic industries due to their unique properties compared with traditional methods involving breaking down rice into smaller parts called floury seeds.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Broken rice is pulverized to particle size and is 185 mesh broken rice powders by superfine pulverizer.

[0025] Add 6.55 kg of water to 1 kg of broken rice flour, and adjust the pH to 7.6 to 7.8 through sodium bicarbonate.

[0026] Add protease enzyme preparation in the preparation liquid, the addition amount of protease enzyme preparation is 151g preparation liquid, and protease enzyme preparation is amylase and cellulase, and both enzyme activities are respectively 30U / g, 10U / g, both weight ratios are 1 : 1, the temperature of the enzymolysis reaction was controlled at 52.8°C, and the enzymolysis was maintained for 12 hours.

[0027] Centrifuge to obtain filter cake and filtrate.

[0028] The filtrate was inactivated at 100°C, then concentrated under reduced pressure at 42°C and -0.04 to -0.01Mpa. When the concentration of the fixed substance was 18%, the vacuum was broken, and then spray-dried until the water content was below 5%. Promptly get 62.9g rice protein, ...

Embodiment 2

[0031] Broken rice is pulverized to particle size and is the broken rice powder of 200 meshes by superfine pulverizer.

[0032] Add 6.45 kg of water to 1 kg of broken rice flour, and adjust the pH to 7.6 to 7.8 through sodium bicarbonate.

[0033] Add protease enzyme preparation in the preparation liquid, the addition amount of protease enzyme preparation is 111.75g ​​preparation liquid, and protease enzyme preparation is amylase and cellulase, both enzyme activities are respectively 30U / g, 10U / g, both weight ratios are 1:1.3, the enzymolysis reaction temperature was controlled at 52.6°C, and the enzymolysis was maintained for 16 hours.

[0034] Centrifuge to obtain filter cake and filtrate.

[0035] The filtrate was inactivated at 100°C, then concentrated under reduced pressure at 43°C and -0.06 to -0.04Mpa. When the concentration of the fixed substance was 19%, the vacuum was broken, and then spray-dried until the water content was below 5%. That is, 64.5 g of rice protein...

Embodiment 3

[0038] The broken rice is pulverized to a particle size of 250 mesh broken rice flour by an ultrafine pulverizer.

[0039] Add 6.35 kg of water to 1 kg of broken rice flour, and adjust the pH to 7.6 to 7.8 through sodium carbonate.

[0040] Add protease enzyme preparation in the preparation liquid, the addition amount of protease enzyme preparation is 36.75g preparation liquid, and protease enzyme preparation is amylase and cellulase, and both enzyme activities are respectively 30U / g, 10U / g, both weight ratios are 1:0.8, the enzymolysis reaction temperature was controlled at 52.5°C, and the enzymolysis was maintained for 14 hours.

[0041] Centrifuge to obtain filter cake and filtrate.

[0042] The filtrate was inactivated at 100°C, then concentrated under reduced pressure at 45°C and -0.08 to -0.06Mpa. When the concentration of the fixed substance was 18.5%, the vacuum was broken, and then spray-dried until the water content was below 5%. That is, 63.5 g of rice protein is ...

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Abstract

The invention discloses broken rice deep processing technology, and particularly relates to processing technology for extracting protein by a broken rice enzyme method. Broken rice is subject to superfine grinding, and added with water and sodium bicarbonate; the mixture is blended and carries out an enzymolysis reaction; solid-liquid separation is performed to prepare rice starch and rice protein. The protease preparations used in the enzymolysis reaction are amylase and cellulase, and the enzyme activities of the two are 30 U/g and 10 U/g respectively. The processing technology for extracting protein by a broken rice enzyme method of the invention performs degradation and modification of rice protein by using the protease preparations, and converts the rice protein into soluble peptide which is then extracted. The extraction reaction condition is mild; the protein polypeptide chain can be hydrolyzed into short peptide chains, which both increases the protein dissolvability and the solid content in the extract, and reduces energy consumption for removing water in the extract. Meanwhile, the protein recovery rate is up to 85%; the broken rice total utilization rate is up to more than 55%; full utilization of the broken rice resources is realized; and important effect on the integration of national grain resources is provided.

Description

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Claims

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Application Information

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Owner 安徽省旌德县辉煌粮油有限责任公司
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