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Method for preparing peanut peptide by fractional bienzyme hydrolysis

A step-by-step hydrolysis and peanut peptide technology, which is applied in the field of peanut protein processing, can solve the problems of high peanut peptide preparation cost, low peanut peptide yield, and long preparation time, and achieve short preparation time, strong functionality, and simple preparation method Effect

Inactive Publication Date: 2011-04-06
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is to provide a method for preparing peanut peptide by double-enzyme step-by-step hydrolysis, so as to overcome the disadvantages of high production cost of peanut peptide, low yield of peanut peptide, low purity, and long preparation time under the existing conditions.

Method used

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  • Method for preparing peanut peptide by fractional bienzyme hydrolysis
  • Method for preparing peanut peptide by fractional bienzyme hydrolysis
  • Method for preparing peanut peptide by fractional bienzyme hydrolysis

Examples

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Effect test

Embodiment 1

[0020] A method for preparing peanut peptides by stepwise hydrolysis with two enzymes, characterized in that it comprises the following steps:

[0021] Step 1: Take peanut protein isolate powder and add distilled water to dissolve it, then put it in a constant temperature water bath at 9°C for 20 minutes to passivate the peanut protein isolate;

[0022] The second step: after the first passivation is completed, take it out, adjust the pH value of the protein solution to 8.0, add endonuclease, and react in a constant temperature water bath shaker at 5°C for 120 minutes;

[0023] Step 3: After the reaction in the second step, adjust the pH value of the protein solution, add flavor protease and mix evenly, react in a constant temperature water bath oscillator at a certain temperature for a period of time, take it out immediately after the reaction, and put it in a water bath at 1°C to keep After 5 minutes, the enzyme was inactivated, cooled with cold water, and the peanut peptide...

Embodiment 2

[0028] A method for preparing peanut peptides by stepwise hydrolysis with two enzymes, characterized in that it comprises the following steps:

[0029] Step 1: Take peanut protein isolate powder and add distilled water to dissolve it, then put it in a constant temperature water bath at 9°C for 20 minutes to passivate the peanut protein isolate;

[0030] The second step: after the first passivation is completed, take it out, adjust the pH value of the protein solution to 8.0, add endonuclease, and react in a constant temperature water bath shaker at 5°C for 120 minutes;

[0031] Step 3: After the reaction in the second step, adjust the pH value of the protein solution, add flavor protease and mix evenly, react in a constant temperature water bath oscillator at a certain temperature for a period of time, take it out immediately after the reaction, and put it in a water bath at 1°C to keep After 5 minutes, the enzyme was inactivated, cooled with cold water, and the peanut peptide...

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Abstract

The invention relates to a method for preparing peanut peptide by fractional bienzyme hydrolysis, belonging to the technical field of peanut protein processing methods. The method is characterized by comprising the following steps of: dissolving the peanut protein powder in distilled water, then placing the dissolved peanut protein powder into a 9 DEG C constant-temperature water bath, and preserving for 20min so as to passivate separated peanut protein; taking out the separated peanut protein after passivation, regulating the pH value of the protein solution to be 8.0, adding incision enzyme, and reacting for 120min in a 5 DEG C constant-temperature water bath oscillator; after reaction, regulating the pH value of the protein solution, adding flavourzyme, evenly mixing, reacting for a certain period of time in a constant-temperature water bath oscillator with a certain temperature, taking out after the reaction, placing into a 1 DEG C water bath, preserving for 5min, and cooling with cold water to obtain the peanut peptide after cooling. The invention has the advantages of low preparation cost, high peanut peptide yield, high purity, short preparation time, simple preparation method, and mild production conditions, and the mass concentration of soluble nitrogen in an enzymolysis product is high.

Description

technical field [0001] The invention belongs to the technical field of peanut protein processing methods, and relates to a method for preparing peanut peptides through double-enzyme step-by-step hydrolysis. Background technique [0002] Peanut is an important oil crop and edible crop in my country, and its output and export volume rank first in the world. At present, China's utilization of peanuts is mostly in the extraction of edible oils, and the utilization of peanut proteins is less. The protein content of peanuts is 25% to 30%. Peanut protein contains eight kinds of amino acids necessary for the human body. The content of arginine is higher than that of other nuts, and its biological value is higher than that of soybeans. At present, the peanut protein products on the domestic market are mainly peanut protein powder, which is generally used as the basic raw material in food processing. Peanut protein powder has disadvantages such as difficult absorption and poor physi...

Claims

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Application Information

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IPC IPC(8): A23J3/34
Inventor 杨庆利于丽娜高俊安孙杰张初署朱凤毕杰
Owner SHANDONG PEANUT RES INST
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