Digital microarray organ chip and application thereof

Abstract

The invention provides a digital microarray organ chip. The digital microarray organ chip comprises a top plate, a PET film and a bottom plate. An upper chamber is formed in the top plate, a lower chamber is formed in the bottom plate, and the upper chamber and the lower chamber are consistent in shape and are separated through the PET film; microarray holes used for microsphere culturing and digital detecting are arrayed in the lower chamber of the bottom plate; and an input pipeline and an output pipeline are connected to the top plate and communicate with the upper chamber. The digital microarray organ chip has the characteristics of being biomimetic, high in throughput and low in cost, and has good prospects in the aspects of clinical application of drug screening, disease modeling, cytotoxicity testing and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a digital microarray organ chip based on microfluidic technology and its application. Background technique [0002] The "organ-on-a-chip" technology is to cultivate living cells in a microfluidic device by continuous perfusion to simulate the physiological functions of tissues and organs. It can provide a simulated physical environment (fluid shear force, cyclic strain and mechanical compression, etc.) for cell growth, making it a more realistic representation of cells and organs. The ability to control fluid flow within an organ-on-a-chip enhances the differentiation and long-term maintenance of function of many cell types. Existing organ chips can use microfluidic technology, microfabrication and biomaterials to simulate the complex three-dimensional microenvironment in the body. aisle. It is also possible to mimic the biological tumor phenotype observed in vivo, namely a gradie...

AI Technical Summary

Problems solved by technology

Based on the digital analysis of microdroplet mode, it is difficult to remove toxic metabolites and add nutrients produced in the process of cell growth, which greatly limits the research and application of microdroplet analysis platform in organ chips

Although the microwell-based microarray organ chip technology has a rudimentary form of digital analysis, the current microarray organ chip is used to trap cells in the chip and culture them in clusters, which all face the disadvantage of low throughput.

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