25 results about "Cytotoxicity test" patented technology

The invention relates to preparation of 10-hydroxycamptothecine modified polypeptide and a research on cytotoxicity of the 10-hydroxycamptothecine modified polypeptide. The method comprises the following steps: synthesizing SA-7 polypeptide by adopting an F-moc solid-phase synthesis method, and combining the SA-7 polypeptide with 10-hydroxycamptothecine through chemical coupling reaction to obtain the SA-7-HCPT compound. C18 reversed-phase high performance liquid chromatography (RP-HPLC) is used for purification, and the molecular weight is identified to be 1898Da by using mass spectrum. A further cytotoxicity test shows that the SA-7-HCPT shows relatively strong anticancer activity to HepG2, and the toxicity of the SA-7-HCPT to HepG2 is remarkably superior to that of 10-HCPT to HepG2. Meanwhile, the toxicity of SA-7-HCPT to normal liver cells LO2 is relatively low, and the SA-7-HCPT shows good selectivity. Therefore, the SA-7-HCPT has a relatively good clinical application prospect.

The invention discloses an MAGE-A10 specific T cell receptor (MAGE-A10TCR) and an application of the MAGE-A10 specific T cell receptor in immunotherapy, and belongs to the technical field of tumor immunotherapy. The MAGE-A10TCR is screened from a tumor reactive T cell of an HCC patient, can specifically recognize an MAGE-A10 antigen and is used for constructing the MAGE-A10TCR-T cell, and a cytotoxicity test shows that the MAGE-A10TCR-T cell has a clearing effect on the HCC cell.

The invention relates to a composition for rapidly fading red and repairing sensitive skin, and belongs to the technical field of skin care products. The composition for rapidly fading red and repairing sensitive skin is prepared from the following raw materials in parts by mass: 20 to 30 parts of active substance gel, 0.2 to 0.5 part of collagen, 1 to 2 parts of glycerol, 0.5 to 0.7 part of fructus kochiae and 50 to 60 parts of water, the active substance gel is prepared from the following active substances in parts by weight: 1.5 to 3 parts of N-acetylneuraminic acid, 1.2 to 2.5 parts of leonurine derivative and 1.2 to 1.5 parts of schefflera octophylla extract. The in-vitro cytotoxicity test of the composition for rapidly fading red and repairing sensitive skin verifies the safety of the composition acting in vitro; when acting on the skin of a sensitive skin patient, the traditional Chinese medicine composition can obviously relieve swelling and pain, and can achieve the purpose of repairing the skin barrier.

The present invention discloses a lipid droplet-targeted bifunctional ratiometric fluorescent probe and its application in the preparation of a •OH detection reagent, wherein the structure of the bifunctional ratiometric fluorescent probe is as follows: #imgabs0#. The bifunctional ratiometric fluorescent probe of the present invention can respond to different concentrations of •OH with ratiometric fluorescence, with a detection limit as low as 1.18nM and a difference between the two emission peaks as high as 140 nm, enabling dual-channel detection without crosstalk. Cytotoxicity tests have shown that the probe has good biocompatibility, and confocal fluorescence microscopy experiments have shown that the probe has good photostability for HepG2 cells, has the ability to monitor changes in endogenous and exogenous •OH concentrations in cells through dual channels, and has the ability to image lipid droplets (R 2 =0.85), which is suitable for confocal fluorescence imaging of intracellular lipid droplet tracking.

This invention discloses a method and system for cell viability detection using dual-channel differential dynamic adhesion impedance analysis. The system utilizes a microfluidic chip integrating detection and reference channels to synchronously apply fluid disturbances to both channels, inducing a dynamic process of "adhesion → detachment → re-adhesion" in cells. During this process, the differential impedance curve (after background subtraction) is acquired in real time, and six dynamic feature parameters, including the amplitude and rate of decrease and recovery, are extracted. A ridge regression algorithm is used to construct a cell viability prediction model, and the six dynamic feature parameters are input into the model to obtain cell viability values. This invention effectively eliminates environmental and fluid common-mode noise through differential structure and achieves label-free, in-situ, highly sensitive measurement of cell viability using impedance analysis. It has advantages such as accurate results, good stability, and high automation, and is suitable for fields such as cytotoxicity testing and drug screening.

The invention discloses application of neochlorogenic acid in preparation of a medicine for preventing and / or treating hand-foot-and-mouth disease caused by A16 type coxsackie virus (CVA16) infection, and belongs to the technical field of biological medicine. Researches prove that the neochlorogenic acid has the effect of resisting CVA16 infection on HEK293T cells and H4 cells, after the two cells treated by the neochlorogenic acid are infected by the CVA16, the viral genome RNA abundance is obviously reduced, and the cell lesion death condition is obviously relieved; meanwhile, a cytotoxicity test shows that the neochlorogenic acid has no obvious cytotoxicity to HEK293T cells and only has weak cytotoxicity to H4 cells. Therefore, in a reasonable dosage range, the neochlorogenic acid, which is a low-toxicity natural small molecule compound, can be used for preparing the anti-CVA16 medicine for preventing and / or treating the hand-foot-and-mouth disease caused by CVA16 infection.

The invention provides low-toxicity and low-sensitization fluororubber watchband glue as well as a preparation method and application thereof, and belongs to the technical field of rubber materials. According to the technical scheme of the invention, the raw fluororubber is polymerized by adopting the surfactant containing no fluorine element, and meanwhile, the reinforcing filler system is optimized, so that the skin sensitization and stimulation risks are remarkably reduced. The fluororubber watchband adhesive prepared by the preparation method disclosed by the invention shows excellent biological safety in a cytotoxicity test. The invention provides a novel environment-friendly material solution with safety and reliability for the field of wearable equipment, particularly watchband products in long-term contact with human skin, and has definite market application prospect and popularization value.

The present invention discloses a two-photon fluorescent probe and its application in sulfatase detection, wherein the structure of the endoplasmic reticulum-targeted two-photon fluorescent probe is shown below: #imgabs0#. The endoplasmic reticulum-targeted two-photon fluorescent probe of the present invention responds to different concentrations of sulfatase, with a detection limit as low as 0.12 U / L and a two-photon absorption cross section of 81 GM. Cytotoxicity tests demonstrate that the probe has low biological toxicity. Confocal fluorescence microscopy imaging demonstrates that the probe can effectively target the endoplasmic reticulum in HeLa cells (localization coefficient of 0.86), making it suitable for fluorescent imaging detection of sulfatase in the endoplasmic reticulum of living cells.

The invention discloses a two-photon fluorescent probe and application thereof in sulfatase detection. The structure of the endoplasmic reticulum targeting two-photon fluorescent probe is shown as follows: # imgabs0 #. The endoplasmic reticulum targeting two-photon fluorescent probe responds to sulfatase with different concentrations, the detection limit is as low as 0.12 U / L, and the two-photon absorption cross section is 81 GM. A cytotoxicity test shows that the probe is relatively low in biotoxicity. Confocal fluorescence microscopic imaging shows that the probe can effectively target the endoplasmic reticulum (the positioning coefficient is 0.86) in HeLa cells, and is suitable for fluorescence imaging detection of sulfatase in the endoplasmic reticulum of living cells.

The invention relates to the field of deep processing of cornus officinalis kernels, in particular to a cornus officinalis kernel extract with high biocompatibility as well as a preparation method and application of the cornus officinalis kernel extract. The method comprises the following steps: crushing dried cornus officinalis kernels, and performing reflux extraction with an absolute ethyl alcohol solution at 80 DEG C for 3 times, 2 hours each time; and combining the extracting solutions, filtering, carrying out vacuum concentration on the suction filtrate by using a rotary evaporator, and freeze-drying to obtain the dogwood kernel extract. The dogwood kernel component has a test concentration of 0.125-4 mg / mL, and has an obvious free radical scavenging effect, and the scavenging rate is enhanced along with the increase of the concentration and can reach 75% at most; in a CCK-8 method cytotoxicity test, the cell survival rate of the culture medium PC12 containing the cornus officinalis kernel component is 100% or above; the research proves that the dogwood kernel extract has better antioxidant activity, neuroprotective effect and cell safety, and provides a basis for further development and utilization of dogwood kernels.

The application provides a melt-blown non-woven fabric and a preparation method and application thereof. The first aspect of the application provides a preparation method of a melt-blown non-woven fabric, wherein polypropylene resin is used as a base, nano-silver wires and an electret are added, and then melt extrusion, melt-blown spinning and electret treatment are sequentially performed to obtain an electret melt-blown non-woven fabric; and the electret melt-blown non-woven fabric is subjected to subsequent treatment to obtain a melt-blown non-woven fabric. The melt-blown non-woven fabric provided by the application has good antiviral effect, and the results of multiple complete skin irritation tests show that the skin irritation index of the non-woven fabric is 0, and the skin irritation intensity is non-irritating; in-vitro cytotoxicity test detection shows that the non-woven fabric has no cytotoxicity. Therefore, the melt-blown non-woven fabric provided by the application can be applied to the fields of clothing, medical treatment, industry and the like, and can be particularly applied to the preparation of protective products.

The invention discloses a smoke particulate matter trapping method and a tobacco product in-vitro cytotoxicity testing method, and belongs to the technical field of smoke toxicity analysis. The smoke particulate matter trapping method comprises the following steps: collecting an atomized product generated by smoking a smoke product by adopting a filter disc to form a trapped filter disc; the number of suction ports corresponding to each filter disc is 200 to 400; and carrying out extrusion treatment on the captured filter disc to form a treatment mother solution containing flue gas particulate matters. According to the method, the flue gas particulate matters are collected by adopting the filter disc in a supersaturation manner, the treated mother liquor containing the flue gas particulate matters with high concentration can be obtained through extrusion treatment, and the flue gas particulate matter components of the treated mother liquor are basically consistent with those of the flue gas particulate matters captured by a traditional Cambridge filter disc method; the preparation of the high-concentration smoke particulate matter working solution can be realized, the in-vitro cytotoxicity of tobacco products can be effectively evaluated, and the IC50 value can be obtained.

The application discloses a MAGE-A10 specific T cell receptor (MAGE-A10 TCR) and application thereof in immunotherapy, and belongs to the technical field of tumor immunotherapy. The MAGE-A10 TCR is screened from tumor reactive T cells of HCC patients, can specifically recognize MAGE-A10 antigens, and is used for constructing MAGE-A10 TCR-T cells. Cytotoxicity tests show that the MAGE-A10 TCR-T cells have a clearing effect on HCC cells.

This invention discloses the application of dermal ethylsin in the preparation of antibacterial and repairing cosmetics or pharmaceuticals, and compositions containing it. Dermal ethylsin can inhibit Propionibacterium acnes, Staphylococcus aureus, and / or Staphylococcus epidermidis, and can promote keratinocyte migration and / or upregulate the expression of repair-related genes. Cytotoxicity tests show that dermal ethylsin has good safety. Human efficacy evaluation shows that serums containing dermal ethylsin can significantly reduce inflammatory papules, improve skin redness, and improve barrier function. Therefore, dermal ethylsin can be used as a dual-action antibacterial and repairing active agent in cosmetics or pharmaceuticals for acne skin care, sensitive skin repair, and / or post-medical aesthetic repair. Dermal ethylsin is derived from *Gnaphalium affine*, has good safety, and is non-irritating to the skin.

The invention discloses an eucalyptol type sesquiterpene lactone TBA and TBB nitrogen mustard derivative as well as a preparation method and application thereof. The structural formulas of the eucalyptol type sesquiterpene lactone TBA and TBB nitrogen mustard derivative are respectively shown as a formula TBA-DJ-1-3 and a formula TBB-DJ-1-3. Cytotoxicity tests show that the nitrogen mustard derivatives can inhibit the proliferation activity of liver cancer cells, particularly, the proliferation inhibition effect of the compound TBA-DJ-2 on HepG2 cancer cells is remarkably better than that of a parent body, and the nitrogen mustard derivatives can be used for clinically preparing anti-liver cancer drugs.

The invention is applicable to the technical field of biological medicines, and provides a pharmaceutical composition for reducing toxicity of chemotherapeutic drugs and application of the pharmaceutical composition, and application of lipoic acid or pharmaceutically acceptable derivatives thereof in preparation of drugs for reducing toxicity of chemotherapeutic drugs. A cytotoxicity test, a cell viability test, a flow cytometry and the like verify that the lipoic acid or the derivative lipoic amide thereof can inhibit the toxicity of chemotherapeutic drugs to cells and reduce pyroptosis caused by the chemotherapeutic drugs; in-vivo experiments of mice prove that the lipoic acid or the derivative lipoic amide thereof can significantly reduce the toxicity of chemotherapeutic drugs such as cis-platinum and the like to various tissues and organs in the mice, and has wide medical application.

The invention provides an acylhydrazone-Mn complex as well as a preparation method and application thereof. The structural formula of the acylhydrazone-Mn complex is shown in the specification, or in the structural formula, R is a substituent group. According to the invention, 4-diphenylaminobenzoylhydrazone reacts with MnCl2, the acylhydrazone-Mn complex is obtained by utilizing easy formation, strong coordination ability, diverse applications and biological activity of acylhydrazone compounds and utilizing N, O atoms and a plurality of metal ions to form complexes with different structures and properties, and some complexes have the characteristics of anti-tumor and antibacterial activity. A cytotoxicity test, an in-vitro proliferation inhibition activity experiment and an antibacterial experiment show that the synthesized Mn complex not only has a relatively good inhibition effect on tumor cells, but also has relatively low toxicity on normal cells; in addition, the proliferation of P.micro can be effectively inhibited.

The invention discloses a ratiometric fluorescent probe responding to NADH (Nicotinamide Adenine Dinucleotide) as well as a preparation method and application of the ratiometric fluorescent probe. The structure of the ratiometric fluorescent probe responding to the NADH is as follows: # imgabs0 #. The ratio fluorescent probe responding to the NADH shows specific ratio fluorescent response to the NADH at 460 nm and 590 nm, and the detection limit of the ratio fluorescent probe is as low as 42 nM. Cytotoxicity tests show that the fluorescent probe is low in biotoxicity, confocal fluorescence microscopic imaging experiments show that the fluorescent probe is good in light stability in HeLa cells, NADH level changes can be sensitively detected through ratio fluorescence imaging, cancer cells and normal cells are effectively distinguished, the fluorescent probe is suitable for ratio fluorescence imaging and detection of the NADH in the cells, and the fluorescent probe has good application prospects. Tumor distribution can be quickly and accurately visualized in an in-situ spraying mode, and the method is used for fluorescent surgical navigation.

The invention discloses a lysosome targeted two-photon fluorescent probe and application thereof in beta-galactosidase detection. The structure of the fluorescent probe is shown as follows: # imgabs0 #. The two-photon fluorescent probe can respond to beta-galactosidase (beta-gal) with different concentrations, and the detection limit is as low as 0.16 U / L. A cytotoxicity test shows that the probe is good in biocompatibility, a confocal fluorescence microscopic imaging experiment shows that the probe is good in cell light stability, has the capacity of monitoring endogenous and exogenous beta-gal concentration changes of cells, has lysosome targeting capacity (R2 = 0.89), and can be used for detecting the endogenous and exogenous beta-gal concentration changes of the cells. The method is suitable for confocal fluorescence imaging of beta-gal concentration change tracing in cytolysosome.

The application discloses a wear-resistant vortex spinning core-spun yarn and a preparation method thereof. The core-spun yarn comprises a core yarn and a wrapping yarn, the mass of the core yarn is 30-50% of the mass of the wrapping yarn; the core yarn is zinc-modified cotton yarn obtained through vortex spinning of zinc-modified cotton fibers; and the wrapping yarn is an antibacterial wear-resistant agent modified cotton yarn obtained through vortex spinning of the antibacterial wear-resistant agent modified cotton fibers. The zinc-modified cotton yarn is used as the core yarn, the antibacterial wear-resistant agent modified cotton yarn is used as the wrapping yarn, and the wear-resistant vortex spinning core-spun yarn is prepared through vortex spinning, so that the wear resistance and the antibacterial property of the core-spun yarn product are ensured due to the stable combination of zinc and the antibacterial wear-resistant agent. The cytotoxicity test result also shows that the core-spun yarn product of the application does not obviously produce toxicity to organisms.

The present invention relates to a method for screening for drugs for prevention or treatment of type 2 diabetes mellitus by using an islet organoid and, more specifically, to a method by which drugs that are effective in the prevention or treatment of type 2 diabetes mellitus can be screen for from candidate substances by using an islet organoid, and a platform therefor. The method for screening for drugs for prevention or treatment of type 2 diabetes mellitus by using an islet organoid, according to the present invention, provides a platform and a method using multi-wells of at least 96 wells so as to rapidly screen for new drug candidate substances that are effective in the prevention or treatment of type 2 diabetes mellitus by using an islet organoid having a cell composition and physical structure more similar to those of the actual human body. The screening method according to the present invention enables simultaneous deriving of the results of 1) a hormone stimulation test, 2) an inflammatory stress test, and 3) a cytotoxicity test on candidate substances for treatment of type 2 diabetes mellitus through merely a single experiment.

The invention discloses a mitochondrial targeting two-photon fluorescent probe and application thereof in ClO <-> detection. The structure of the two-photon fluorescent probe is shown as follows: # imgabs0 #. The mitochondria targeting two-photon fluorescent probe responds to ClO <-> with different concentrations, the reaction speed is high (30 s), the detection limit is as low as 0.32 mu M, the probe has the two-photon absorption property, and the maximum two-photon absorption cross section at 840 nm is 41.2 GM. A cytotoxicity test shows that the probe is good in biocompatibility, a confocal fluorescence microscopic imaging experiment shows that the probe is good in light stability, has the capacity of monitoring endogenous and exogenous ClO <-> concentration changes of cells, has the mitochondrial targeting capacity (R2 = 0.82), and can be used for detecting the mitochondrial targeting of the cells. A confocal fluorescence microscopic imaging experiment can detect that an exothermic reaction occurs after the probe responds to ClO <-> in cells to generate various active oxygen (OH., O2 and < 1 > O2), and the probe has a certain biological application value.

The application discloses a kind of detection tyrosinase dual-channel near-infrared ratio fluorescent probe and its preparation method and purposes, wherein the structure of detection tyrosinase dual-channel near-infrared ratio fluorescent probe is as follows: the dual-channel near-infrared ratio fluorescent probe LZD of the application shows good selectivity and sensitive dual-channel fluorescence signal to tyrosinase.Cytotoxicity test shows that the ratio probe has good biocompatibility, and confocal fluorescence microscopic imaging experiment shows that the ratio probe has good light stability to HepG2 cells, can respond to tyrosinase through dual-channel fluorescence, and can carry out confocal fluorescence imaging to extracellular and endogenous tyrosinase of cell.

The invention belongs to the technical field of medicines. Relates to a novel styrene lactone compound and application thereof in pharmacy, the structural formulas of the compounds are shown as a compound 1 and a compound 2, the antitumor effect of the novel styrene lactone compound on cytotoxicity tests of various colon cancer cells is included, and the result shows that the novel styrene lactone compound has a good anti-tumor effect on various colon cancer cells. The compound shows a remarkable growth inhibition effect on human colon cancer cell lines LS513 and SW620, the IC50 value range of the compound is 1.6-3.9 [mu] M, and the result is superior to 32-84 [mu] M of a positive control drug 5-FU. Meanwhile, experiments show that the compounds 1 and 2 do not show cytotoxicity to human embryo kidney normal cells HEK293T at 100 [mu] M, and the compounds can be used for preparing drugs for treating colon cancer. # imgabs0 #

The invention relates to the field of medicines, and discloses application of cordycepin to preparation of a medicine for treating babesia infection and a pharmaceutical composition of the cordycepin. The invention relates to application of cordycepin in preparation of a medicine for treating babesia infection. The composition contains a therapeutically effective amount of cordycepin and pharmaceutically acceptable carriers or auxiliary materials, can be prepared into dosage forms such as tablets, granules, capsules, oral liquid, injections or suspensions, and is suitable for administration modes such as oral administration. In-vitro evaluation shows that the cordycepin has inhibitory activity on Babesia bovis and Babesia gibsoni, and the median inhibitory concentrations (Ic50) of the cordycepin and the Babesia gibsoni are respectively about 303.8 Nm and 147.5 Nm; in a mouse babesia vole infection model, the parasitic rate peak value can be obviously reduced and delayed through oral administration of 20 Mg / Kg (the model group is about 17.5% / 13th day, and the cordycepin group is about 1.8% / 17th day). A cytotoxicity test (Hff-1, Cck-8) shows that the inhibition rate on cell proliferation under the condition of 100 microns is less than 10%. The cordycepin and the pharmaceutical composition thereof can be used for preventing and / or treating babesia infection.