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Natural polymer-based nano-fibrous membrane prepared by freeze-drying method

A natural polymer and nanofiber membrane technology, applied in fiber treatment, spinning solution preparation, fiber chemical characteristics, etc., can solve the troublesome post-processing of the preparation method, high requirements for process factors and environmental factors, and poor repeatability And other issues

Inactive Publication Date: 2012-03-21
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In view of the defects that the natural polymer nanofibers currently prepared have high requirements on process factors and environmental factors, the post-processing of the preparation method is troublesome, and the repeatability is not strong. The purpose of the present invention is to provide a method for preparing natural polymer nanofiber membranes. Methods

Method used

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  • Natural polymer-based nano-fibrous membrane prepared by freeze-drying method
  • Natural polymer-based nano-fibrous membrane prepared by freeze-drying method
  • Natural polymer-based nano-fibrous membrane prepared by freeze-drying method

Examples

Experimental program
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Effect test

Embodiment 1

[0025] (1) Sodium alginate is dissolved in deionized water to prepare 20mL of a solution with a weight percentage of 0.001wt%, and then the solution is fully stirred so that it is completely dissolved to form a sodium alginate solution.

[0026] (2) Transfer the sodium alginate solution prepared in step (1) to a liquid nitrogen freezer, turn on the freezer, freeze the prepared sodium alginate solution rapidly in a liquid nitrogen environment, and then freeze at -80°C and under the condition that the degree of vacuum is 600 Pa, the frozen sodium alginate solution is subjected to freeze-drying treatment in a freeze dryer for 24 hours to obtain sodium alginate nanofibers.

[0027] (3) The sodium alginate nanofibers obtained in step (2) were crosslinked with 10mmol / L calcium chloride for 2 hours at 25°C, and the excess chlorine in the crosslinked sodium alginate nanofibers was Calcium was washed away with deionized water, and the treated nanofiber nonwoven was vacuum-dried at 60 °...

Embodiment 2

[0031] (1) Dissolve hyaluronic acid in deionized water to prepare 20mL of a solution with a weight percentage of 0.015wt%, and then stir the solution fully so that it is completely dissolved to form a hyaluronic acid solution.

[0032] (2) Transfer the hyaluronic acid solution prepared in step (1) to a liquid nitrogen freezing device, turn on the freezing device, and rapidly freeze the prepared hyaluronic acid solution in a liquid nitrogen environment, and then the freezing temperature is -10°C and under the condition that the degree of vacuum is 200 Pa, the frozen hyaluronic acid solution was freeze-dried in a freeze dryer for 12 hours to obtain hyaluronic acid nanofibers.

[0033] (3) The hyaluronic acid nanofibers obtained in step (2) were crosslinked with 5 mmol / L carbodiimide for 2 hours at 20°C, and the excess hyaluronic acid nanofibers after crosslinking The carbodiimide was washed away with deionized water, and the treated nanofiber non-woven fabric was vacuum-dried at...

Embodiment 3

[0037] (1) Chitosan is dissolved in 1wt% acetic acid solution to prepare 20mL of a 0.1wt% solution by weight, and then the solution is fully stirred so that it is completely dissolved and the chitosan solution is obtained.

[0038] (2) The chitosan solution prepared in step (1) is transferred to a liquid nitrogen freezer, the freezer is opened, and the prepared chitosan solution is rapidly frozen in a liquid nitrogen environment, and then the freezing temperature is -40°C Under the condition that the degree of vacuum is 1 Pa, the frozen chitosan solution is subjected to freeze-drying treatment in a freeze dryer for 36 hours to obtain chitosan nanofibers.

[0039] (3) The chitosan nanofibers obtained in step (2) were cross-linked for 2 hours with 2mmol / L genipin at 40°C, and the excess genipin in the crosslinked chitosan nanofibers was Nippin was washed off with absolute ethanol, and the treated nanofiber non-woven fabric was vacuum-dried at 50 for 6h to obtain a chitosan nanof...

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Abstract

The invention relates a biodegradable and absorbable natural polymer-based nano-fibrous membrane prepared by a freeze-drying method, and the application thereof. The natural polymer-based nano-fibrous membrane is prepared through the following steps of: dissolving natural polymer powder into a corresponding solvent so as to prepare an extremely-dilute solution with the concentration of 0.001-0.1wt %; after the natural polymer powder is completely dissolved in the solvent, transferring the obtained natural polymer solution into a liquid nitrogen refrigerating device, so that the natural polymer solution is rapidly frozen in a liquid nitrogen environment; then, carrying out freeze-drying treatment on the obtained product in a freeze drier for 12-48 hours to obtain natural polymer-based nano fibers; and carrying out cross-linking on the obtained natural polymer-based nano fibers by a corresponding cross-linking agent to obtain a natural polymer-based nano-fibrous membrane, and then carrying out MTT (methyl thiazolyl tetrazolium) cytotoxicity test and cell vaccination experiments on the natural polymer-based nano-fibrous membrane, with the obtained results showing that the obtained fibrous membrane has no toxicity but has excellent cell adhesion and proliferation properties. The natural polymer-based nano-fibrous membrane disclosed by the invention is simple in the operation process, easy to control and low in cost; and by using the nano-fibrous membrane disclosed by the invention, ultra-fine natural polymer-based nano fibers can be prepared continuously on a large scale.

Description

technical field [0001] The present invention relates to the preparation of biodegradable and absorbable natural macromolecule-based nanofiber membrane by freeze-drying method and its medical application. Background technique [0002] Vacuum freeze-drying technology uses the principle of ice crystal sublimation to directly sublimate the moisture in the frozen material from solid ice to water vapor under high vacuum environmental conditions to dry the material. According to the phase equilibrium theory in chemical thermodynamics, it can be known that at a certain pressure and temperature, a certain phase equilibrium is reached among the three forms of water, and the phase diagram of water ( figure 1 ). The triple point shows the pressure and temperature conditions under which the gas, liquid and solid phases of water coexist. The OA line in the figure is the boundary line between the solid phase and the gas phase, called the sublimation line; the OB line is the boundary line...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D04H1/42D04H1/425D01D1/02D01D5/00D01F9/04D01F9/00D01F2/28D01F4/00D01F4/02
Inventor 马贵平聂俊刘洋朱晓群
Owner BEIJING UNIV OF CHEM TECH
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