Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Structure and preparation method of liver-targeted platinum-loaded nanometer prodrug

A platinum nano, liver-targeting technology, applied in the field of biomedicine, can solve the problems of high toxicity of cancer cells, strong side effects of patients, discount of chemotherapy effect, etc., and achieve enhanced killing ability, good blood stability, and prevention of non-specific adsorption. Effect

Active Publication Date: 2017-01-04
NANKAI UNIV
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Studies have shown that Pt(II) is highly toxic to cancer cells, and when used as chemotherapy drugs, the side effects of patients are relatively strong, which greatly reduces the effect of chemotherapy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Structure and preparation method of liver-targeted platinum-loaded nanometer prodrug
  • Structure and preparation method of liver-targeted platinum-loaded nanometer prodrug
  • Structure and preparation method of liver-targeted platinum-loaded nanometer prodrug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of liver-targeted platinum-loaded nanoprodrugs for liver cancer treatment:

[0032] 1) Tetrabutylammonium alginate (ALG-TBA) preparation: HCl aqueous solution (0.6M, 30mL) was added to ethanol (30mL), ALG-Na (2.0g) was added to the mixed solution, stirred overnight at 4°C ; ALG-H was obtained by suction filtration, washed with ethanol and acetone in turn, and dried overnight at 40°C in vacuum; the dried alginic acid was dispersed in 100mL distilled water, and tetrabutylammonium base (TBAOH) was added dropwise under stirring conditions. When the polymer is completely dissolved, the pH is adjusted to 7.0-10.0, dialyzed, and freeze-dried to obtain white ALG-TBA.

[0033] 2) Cisplatin prodrug dichloro bis (hydroxide) diammine platinum (IV) (cis, trans, cis-[PtCl 2 (OH) 2 (NH3) 2 ]) Preparation method: dry cisplatin cis-[PtCl2(NH3)2] (0.20g, 6.6*10 -4 mol) was dispersed in 5ml of water, and a 10-fold excess of H2O2 (30% w / v, 7.0mL, 6.6*10 -3 mol),...

Embodiment 2

[0041] Example 2: Concentrated stability study of liver-targeted platinum-loaded nano-prodrugs for liver cancer treatment:

[0042] Preparation of liver-targeted platinum-loaded nano-prodrug concentrate: ALG@GA, Pt(IV) obtained in step 7 of Example 1 by centrifugation (GA:Pt(IV):ALG-TBA=1:2 :4) Nanoparticles were centrifuged at 25,000 rpm for 1 hour. After discarding the supernatant, a platinum-loaded drug nanoparticle concentrate with mouse experiment capability was obtained, wherein the concentration of platinum was 140ug. / ml. Ultrasonic disperse and shake the concentrate, the particle size is 160nm, characterized by DLS, see appendix Figure 4 . It is proved that the nanoparticles have good concentration stability.

Embodiment 3

[0043] Example 3: Determination of the release curve of the liver-targeted platinum-loaded nano-prodrug for the treatment of liver cancer:

[0044] Take 1ml of the liver-targeting platinum-loaded nano-prodrug solution and add it to 70ml of PBS buffer solution containing 0mM glutathione (GSH) and 10mM GSH, then take out 2ml of the solution at a fixed time point, and measure its concentration by ICP to obtain the drug in For the release curves under different reducing environments, see the appendix Figure 5 . In the presence of reducing substances, the release of drugs was significantly increased and there was a sustained release effect.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a liver-targeted platinum-loaded nanometer prodrug for treating liver cancers and a preparation method thereof. The liver-targeted platinum-loaded nanometer prodrug is prepared by taking sodium alginate with the good biocompatibility as a framework to be modified with glycyrrhetinic acid (GA) with a liver cancer cell targeting capacity and a tetravalent platinum compound (PtCl2(OH)2(NH3)2) through a self-assembly technique. In-vitro drug releasing and cytotoxicity tests prove that the platinum-loaded drug nanoparticles have the good blood stability and the intracellular slow-release effect and also have the tumor cell killing capacity. The nanometer prodrug is expected to be used for targeting treatment on the liver cancers and has a good application prospect in the biomedical field.

Description

[0001] Technical field: [0002] The invention relates to a liver-targeting platinum-loaded nanometer prodrug and a preparation method thereof. The nanoparticle prepared by the method is suitable for treating liver cancer and belongs to the technical field of biomedicine. [0003] Background technique: [0004] According to the "World Cancer Report" released by the World Health Organization (WHO) in 2014, among all kinds of malignant tumors in the world, the number of deaths from liver cancer ranks second in the world, and the incidence and death of liver cancer from my country It accounts for more than half of the world (OrganizationWH.World Cancer Report 2014.). In my country, liver cancer is the malignant tumor with the highest mortality rate. It has the characteristics of difficulty in early diagnosis, high degree of malignancy, strong invasiveness, and short survival period of patients. Chemotherapy is currently the main intervention for liver cancer besides surgery. Com...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K33/24A61P35/00A61P1/16
Inventor 袁直王蔚王新宇
Owner NANKAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com