1 results about "Smoke exposure" patented technology

This invention relates to the field of biomedical technology, and in particular to an animal model of COPD combined with sarcopenia and the application of mesenchymal stem cells in the treatment of this disease. The method of constructing the model includes the following steps: (1) Pre-stimulation stage: On the first day of modeling, lipopolysaccharide (LPS) is administered via airway nebulization to initiate airway inflammation, and continuous exposure to cigarette smoke is implemented from the first day of modeling; during this period, LPS is administered again on the 10th to 14th day of modeling to enhance the persistence of inflammation; (2) Partial phenotype confirmation stage: Lung function tests or their alternative indicators are performed on the animals after the pre-stimulation ends; (3) While continuing to implement smoke exposure, dexamethasone proteolytic load and mechanical load regulation treatment are introduced simultaneously for the enrolled animals until the total modeling time reaches 4 to 6 weeks; (4) The endpoint airflow limitation index, weight loss and muscle strength loss index are detected, and the animals are deemed to have successfully constructed the model if they simultaneously meet the corresponding thresholds.