Test method for detecting cytotoxicity of buccal tobacco products

A test method and cytotoxicity technology, applied in the direction of biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems that cannot meet the needs of cytotoxicity detection of snus products, and achieve the effect of accurate and effective detection
CN104789633AActive Publication Date: 2015-07-22CHINA TOBACCO YUNNAN IND
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA TOBACCO YUNNAN IND
Publication Date
2015-07-22

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Abstract

The invention provides a test method for detecting cytotoxicity of buccal tobacco products. The test method comprises the following steps: sample pretreatment, single-cell suspension preparation, cell concentration calculation, cell inoculation, test substance addition, test substance incubation, dye incubation, light absorption value measurement, cell inhibition rate calculation and cell half lethal dose calculation. According to the test method provided by the invention, the exposure means and action manners of the buccal tobacco products are comprehensively surveyed, a sample pretreatment method of the buccal tobacco products is established, human oral mucosa fibroblasts hoMF are selected as the cells for cytotoxicity test of the buccal tobacco products according to the acting target cells, the sample detection dosage is determined and a cytotoxicity test method suitable for the buccal tobacco products is formed.
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Description

technical field

[0001] The invention relates to a test method for detecting the cytotoxicity of snus products, and belongs to the technical field of biological safety evaluation of tobacco and tobacco products. Background technique

[0002] The relative growth rate (RGR) of cells is a key indicator of cytotoxicity evaluation, and it is often used in the classification of cytotoxicity in the development of new drugs and the safety evaluation of medical devices [1，2] . The neutral red method and the thiazolium blue method (MTT) are simple, fast, and sensitive, and they are currently two widely used toxicological detection methods for cytotoxicity. In 2002, CORESTA, the International Tobacco Scientific Research Cooperation Center, established a working group for in vitro toxicological testing of cigarette smoke. Potential cytotoxicity is detected, and this detection method has been widely adopted by domestic and foreign tobacco companies.

[0003] Increasingly stringent toba...

Claims

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