Detection method for cytotoxicity of gas-liquid contact type cigarettes under full-smoke exposure

A technology of cytotoxicity and gas-liquid contact, applied in measuring devices, air quality improvement, instruments, etc., can solve the problems of undiscovered public reports, etc., and achieve the effect of low cost
CN102495183AActive Publication Date: 2012-06-13YUNNAN RES INST OF TOBACCO SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YUNNAN RES INST OF TOBACCO SCI
Publication Date
2012-06-13

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Abstract

The invention relates to a detection method for the cytotoxicity of gas-liquid contact type cigarettes under full-smoke exposure, belonging to the technical field of safety evaluation of cigarette smoke. The detection method provided by the invention comprises the following steps of: firstly, setting cigarette suction parameters to carry out suction on a cigarette; diluting the smoke by clean air with the flow speed of 0-1000 mL / min and transferring the diluted cigarette full smoke into a full-smoke exposure bottle disclosed in the patent with the patent number of ZL201120063251.6 to come into contact and exposure with suspension cells in the bottle, wherein the exposure time is 5 min; after the exposure, carrying out neutral erythrocyte toxicity test or MTT (Methyl Thiazolyl Tetrazolium) cytotoxicity test according to a regular method; analyzing a dosage reaction relation between smoke exposure amount and cytotoxicity according to the following formula: full-smoke dilution multiple=( flow speed of smoke + flow speed of clean air) / flow speed of smoke; smoke exposure amount under exposure of 10 mL of a cell suspension solution for 5 min=TPM (Total Particulate Matter) amount of single cigarette / suction times of single cigarette*6 suctions / 10 mL; and representing the extent of cytotoxicity by a cell inhibition ratio CI. The detection method provided by the invention has the advantages of simplicity, feasibility, low cost and the capability of providing technical supports for the safety evaluation of cigarette smoke.
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Description

Technical field:

[0001] The invention relates to a method for detecting the cytotoxicity of a gas-liquid contact cigarette under full smoke exposure, and belongs to the technical field of cigarette smoke safety evaluation. Background technique:

[0002] The research on the total smoke exposure method for tobacco toxicology evaluation has caused the world because of its irreplaceable advantages in simulating the human body environment, being able to achieve multiple relevant detection endpoints, and detecting the original whole smoke online in real time. The extensive attention of various tobacco companies and analytical testing laboratories, some tobacco companies have conducted in-depth and systematic research on the exposure method.

[0003] At present, there have been patents on the method for external exposure of cigarettes to the whole smoke. Published Patent 1 (application number: 200810230659.0) is a cytotoxicity test method for mainstream cigarette smoke. The main exposure...

Claims

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